Cytotoxic potential of 14 Passiflora species against cancer cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34695 |
Resumo: | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil. |
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Amaral, Ricardo GuimarãesGomes, Silvana Vieira FlorestaLuciano, Maria Claudia dos SantosPessoa, Cláudia do Ó.Andrade, Luciana NaloneSeverino, PatríciaBrandão, Geraldo CélioBomfim, Larissa MendesSoares, Milena Botelho PereiraBezerra, Daniel PereiraDavid, Jorge MaurícioCarvalho, Adriana Andrade2019-08-09T16:53:42Z2019-08-09T16:53:42Z2019AMARAL, Ricardo Guimarães et al. Cytotoxic potential of 14 Passiflora species against cancer cells. Journal of Medicinal Plants Research, v. 13, n. 7, p. 157-166, 2019.1996-0875https://www.arca.fiocruz.br/handle/icict/3469510.5897/JMPR2019.6744engAcademic JournalsCâncerCitotoxicidadeExtrato etanólicoPassifloraPassiflora alataCancerCytotoxicityEthanolic extractPassifloraPassiflora alataCytotoxic potential of 14 Passiflora species against cancer cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil.University of Tiradentes. Institute of Technology and Research. Aracaju, SE, Brazil.Federal University of Ceará. Center for Research and Drug Development. Fortaleza, CE, Brazil.Federal University of Ceará. Center for Research and Drug Development. Fortaleza, CE, Brazil.Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil.University of Tiradentes. Institute of Technology and Research. Aracaju, SE, Brazil.Federal University of Ouro Preto. Department of Pharmacy. Ouro Preto, MG, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia. Institute of Chemistry. Salvador, BA, Brazil.Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil.This work aimed to evaluate the cytotoxic potential against cancer cells of Passiflora genus plant species cultivated in Brazil and identify the mechanism of cytotoxicity induced by the most promising extract. Ethanolic extracts from the leaves of 14 Passiflora species were obtained by accelerated solvent extraction and in vitro cytotoxicity evaluated against cancer cell lines using MTT assay at a single concentration of 50 μg/ml. Additionally, the IC50 of the Passiflora alata (ELPA) leaf extracts was determined against both cancer (HCT-116, SF-295, OVACAR-8, and HL-60), and non-cancer cells (PBMC). The ELPA flavonoids were identified by HPLC-DAD and UHPLC-MS/MS. The morphological analyses, using light and fluorescence microscopy, and cell cycle and DNA fragmentation analysis, using flow cytometry, were evaluated to study the mechanism of cell death induced by ELPA in HL-60 cells. Among the Passiflora leaf extracts evaluated; ELPA stood out with high cytotoxic activity, followed by Passiflora capsularis and Passiflora quadrangularis with varying high and low cytotoxic activity. ELPA presented high cytotoxic potency in HL-60 (IC50 19.37 μg/ml), and without cytotoxicity against PBMC, suggesting selectivity for cancer cells. The cytotoxic activity of ELPA may well be linked to the presence of ten identified flavonoids. Cells treated with ELPA presented the hallmarks typical of apoptosis and necrosis, with cell cycle arrest in the G2/M phase. From among the studied species, ELPA presented greater cytotoxic activity, possibly a consequence of synergistic flavonoid action, which induces cell death by apoptosis and necrosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34695/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAmaral, Ricardo Cytotoxic potential.pdfAmaral, Ricardo Cytotoxic potential.pdfapplication/pdf425517https://www.arca.fiocruz.br/bitstream/icict/34695/2/Amaral%2c%20Ricardo%20Cytotoxic%20potential.pdfd8ab4ea93ef4865459af3cc214eb47ddMD52TEXTAmaral, Ricardo Cytotoxic potential.pdf.txtAmaral, Ricardo Cytotoxic potential.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| title |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| spellingShingle |
Cytotoxic potential of 14 Passiflora species against cancer cells Amaral, Ricardo Guimarães Câncer Citotoxicidade Extrato etanólico Passiflora Passiflora alata Cancer Cytotoxicity Ethanolic extract Passiflora Passiflora alata |
| title_short |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| title_full |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| title_fullStr |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| title_full_unstemmed |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| title_sort |
Cytotoxic potential of 14 Passiflora species against cancer cells |
| author |
Amaral, Ricardo Guimarães |
| author_facet |
Amaral, Ricardo Guimarães Gomes, Silvana Vieira Floresta Luciano, Maria Claudia dos Santos Pessoa, Cláudia do Ó. Andrade, Luciana Nalone Severino, Patrícia Brandão, Geraldo Célio Bomfim, Larissa Mendes Soares, Milena Botelho Pereira Bezerra, Daniel Pereira David, Jorge Maurício Carvalho, Adriana Andrade |
| author_role |
author |
| author2 |
Gomes, Silvana Vieira Floresta Luciano, Maria Claudia dos Santos Pessoa, Cláudia do Ó. Andrade, Luciana Nalone Severino, Patrícia Brandão, Geraldo Célio Bomfim, Larissa Mendes Soares, Milena Botelho Pereira Bezerra, Daniel Pereira David, Jorge Maurício Carvalho, Adriana Andrade |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Amaral, Ricardo Guimarães Gomes, Silvana Vieira Floresta Luciano, Maria Claudia dos Santos Pessoa, Cláudia do Ó. Andrade, Luciana Nalone Severino, Patrícia Brandão, Geraldo Célio Bomfim, Larissa Mendes Soares, Milena Botelho Pereira Bezerra, Daniel Pereira David, Jorge Maurício Carvalho, Adriana Andrade |
| dc.subject.other.pt_BR.fl_str_mv |
Câncer Citotoxicidade Extrato etanólico Passiflora Passiflora alata |
| topic |
Câncer Citotoxicidade Extrato etanólico Passiflora Passiflora alata Cancer Cytotoxicity Ethanolic extract Passiflora Passiflora alata |
| dc.subject.en.pt_BR.fl_str_mv |
Cancer Cytotoxicity Ethanolic extract Passiflora Passiflora alata |
| description |
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil. |
| publishDate |
2019 |
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2019-08-09T16:53:42Z |
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2019-08-09T16:53:42Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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AMARAL, Ricardo Guimarães et al. Cytotoxic potential of 14 Passiflora species against cancer cells. Journal of Medicinal Plants Research, v. 13, n. 7, p. 157-166, 2019. |
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https://www.arca.fiocruz.br/handle/icict/34695 |
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1996-0875 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.5897/JMPR2019.6744 |
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AMARAL, Ricardo Guimarães et al. Cytotoxic potential of 14 Passiflora species against cancer cells. Journal of Medicinal Plants Research, v. 13, n. 7, p. 157-166, 2019. 1996-0875 10.5897/JMPR2019.6744 |
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