Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells

Detalhes bibliográficos
Autor(a) principal: Nogueira, Nadia A. P.
Data de Publicação: 2002
Outros Autores: Grangeiro, Moema B., Cunha, Rodrigo M. S. da, Ramos, Márcio Viana, Alves, Maria A. O., Teixeira, Edson Holanda, Barral Netto, Manoel, Calvete, Juan J, Cavada, Benildo Sousa, Grangeiro, Thalles Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7438
Resumo: Universidade Federal do Ceará. Departamento de Analises Clínicas e Toxicológicas. Fortaleza, CE, Brasil
id CRUZ_2ed575d71ae71abc36e1adc73d502705
oai_identifier_str oai:www.arca.fiocruz.br:icict/7438
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Nogueira, Nadia A. P.Grangeiro, Moema B.Cunha, Rodrigo M. S. daRamos, Márcio VianaAlves, Maria A. O.Teixeira, Edson HolandaBarral Netto, ManoelCalvete, Juan JCavada, Benildo SousaGrangeiro, Thalles Barbosa2014-03-24T16:37:27Z2014-03-24T16:37:27Z2002NOGUEIRA, N. A. P. et al. Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells. Protein and Peptide Letters, v. 9, n. 1, p. 59-66, 2002.0929-8665https://www.arca.fiocruz.br/handle/icict/7438engBentham Science Publishers Ltd.Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Ceará. Departamento de Analises Clínicas e Toxicológicas. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Laboratório de Citogenética e Genética Molecular. Departamento de Biologia. Centro de Ciências. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Laboratório de Citogenética e Genética Molecular. Departamento de Biologia. Centro de Ciências. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Departamento de Biologia. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Laboratório de Citogenética e Genética Molecular. Departamento de Biologia. Centro de Ciências. Fortaleza, CE, BrasilUniversidade Federal do Ceará. BioMol-Laboratório. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilCIB, CSIC, Madrid, SpainUniversidade Federal do Ceará. BioMol-Laboratório. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Laboratório de Citogenética e Genética Molecular. Departamento de Biologia. Centro de Ciências. Fortaleza, CE, BrasilConBr, a D-glucose/D-mannose-specific lectin from Canavalia brasiliensis seeds, was produced in Escherichia coli from a cDNA clone subcloned to pET15b expression vector. The recombinant lectin (rConBr) was purified by onestep immobilized metal-affinity chromatography using an amino-terminal hexahistidine tag. By SDS-PAGE and Western blot, rConBr was highly pure with an apparent molecular mass of 37 kDa. N-terminal sequence analysis revealed a single sequence, confirming the identity of the expressed protein as the pre-pro-ConBr.Fabaceae/genéticaLectinas/genéticaLectinas/metabolismoWestern BlottingClonagem Molecular/métodosEscherichia coli/genéticaEscherichia coli/metabolismoFabaceae/metabolismoExpressão GênicaHistidina/químicaHistidina/metabolismoHumanosLectinas/químicaNíquel/químicaNíquel/metabolismoLectinas de PlantasPlasmídeosProteínas Recombinantesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNogueira N A Expression and Purification....pdfNogueira N A Expression and Purification....pdfapplication/pdf171605https://www.arca.fiocruz.br/bitstream/icict/7438/1/Nogueira%20N%20A%20Expression%20and%20Purification....pdfd6d7da12c44cdb5001ae6efbed408a44MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7438/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTNogueira N A Expression and Purification....pdf.txtNogueira N A Expression and Purification....pdf.txtExtracted texttext/plain20674https://www.arca.fiocruz.br/bitstream/icict/7438/5/Nogueira%20N%20A%20Expression%20and%20Purification....pdf.txt0b2ed9dee3b3b1210ec2b13c2c560002MD55THUMBNAILNogueira N A Expression and Purification....pdf.jpgNogueira N A Expression and Purification....pdf.jpgGenerated Thumbnailimage/jpeg2095https://www.arca.fiocruz.br/bitstream/icict/7438/4/Nogueira%20N%20A%20Expression%20and%20Purification....pdf.jpg9b74e7635f1fc353d4cf2bf510e40dcaMD54icict/74382023-03-15 14:33:22.491oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
title Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
spellingShingle Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
Nogueira, Nadia A. P.
Fabaceae/genética
Lectinas/genética
Lectinas/metabolismo
Western Blotting
Clonagem Molecular/métodos
Escherichia coli/genética
Escherichia coli/metabolismo
Fabaceae/metabolismo
Expressão Gênica
Histidina/química
Histidina/metabolismo
Humanos
Lectinas/química
Níquel/química
Níquel/metabolismo
Lectinas de Plantas
Plasmídeos
Proteínas Recombinantes
title_short Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
title_full Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
title_fullStr Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
title_full_unstemmed Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
title_sort Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
author Nogueira, Nadia A. P.
author_facet Nogueira, Nadia A. P.
Grangeiro, Moema B.
Cunha, Rodrigo M. S. da
Ramos, Márcio Viana
Alves, Maria A. O.
Teixeira, Edson Holanda
Barral Netto, Manoel
Calvete, Juan J
Cavada, Benildo Sousa
Grangeiro, Thalles Barbosa
author_role author
author2 Grangeiro, Moema B.
Cunha, Rodrigo M. S. da
Ramos, Márcio Viana
Alves, Maria A. O.
Teixeira, Edson Holanda
Barral Netto, Manoel
Calvete, Juan J
Cavada, Benildo Sousa
Grangeiro, Thalles Barbosa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Nadia A. P.
Grangeiro, Moema B.
Cunha, Rodrigo M. S. da
Ramos, Márcio Viana
Alves, Maria A. O.
Teixeira, Edson Holanda
Barral Netto, Manoel
Calvete, Juan J
Cavada, Benildo Sousa
Grangeiro, Thalles Barbosa
dc.subject.decs.pt_BR.fl_str_mv Fabaceae/genética
Lectinas/genética
Lectinas/metabolismo
Western Blotting
Clonagem Molecular/métodos
Escherichia coli/genética
Escherichia coli/metabolismo
Fabaceae/metabolismo
Expressão Gênica
Histidina/química
Histidina/metabolismo
Humanos
Lectinas/química
Níquel/química
Níquel/metabolismo
Lectinas de Plantas
Plasmídeos
Proteínas Recombinantes
topic Fabaceae/genética
Lectinas/genética
Lectinas/metabolismo
Western Blotting
Clonagem Molecular/métodos
Escherichia coli/genética
Escherichia coli/metabolismo
Fabaceae/metabolismo
Expressão Gênica
Histidina/química
Histidina/metabolismo
Humanos
Lectinas/química
Níquel/química
Níquel/metabolismo
Lectinas de Plantas
Plasmídeos
Proteínas Recombinantes
description Universidade Federal do Ceará. Departamento de Analises Clínicas e Toxicológicas. Fortaleza, CE, Brasil
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2014-03-24T16:37:27Z
dc.date.available.fl_str_mv 2014-03-24T16:37:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NOGUEIRA, N. A. P. et al. Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells. Protein and Peptide Letters, v. 9, n. 1, p. 59-66, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7438
dc.identifier.issn.none.fl_str_mv 0929-8665
identifier_str_mv NOGUEIRA, N. A. P. et al. Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells. Protein and Peptide Letters, v. 9, n. 1, p. 59-66, 2002.
0929-8665
url https://www.arca.fiocruz.br/handle/icict/7438
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bentham Science Publishers Ltd.
publisher.none.fl_str_mv Bentham Science Publishers Ltd.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7438/1/Nogueira%20N%20A%20Expression%20and%20Purification....pdf
https://www.arca.fiocruz.br/bitstream/icict/7438/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7438/5/Nogueira%20N%20A%20Expression%20and%20Purification....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7438/4/Nogueira%20N%20A%20Expression%20and%20Purification....pdf.jpg
bitstream.checksum.fl_str_mv d6d7da12c44cdb5001ae6efbed408a44
7d48279ffeed55da8dfe2f8e81f3b81f
0b2ed9dee3b3b1210ec2b13c2c560002
9b74e7635f1fc353d4cf2bf510e40dca
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324633317933056

Registros relacionados