Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12922 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação celular. Rio de Janeiro, RJ, Brasil. |
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Ferraz, RaquelCunha, Clarissa F.Silva, Adriano GomesSchubach, Armando O.Pimentel, Maria Inês F.Lyra, Marcelo RosandiskiMendonça, Sérgio C. F.Rosalino, Cláudia M. ValenteCruz, Alda Maria daBertho, Álvaro Luiz2016-03-01T15:29:07Z2016-03-01T15:29:07Z2015FERRAZ, Raquel; et al. Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy. BMC Infectious Diseases, v.15, n.74, 11p., 2015.1471-2334https://www.arca.fiocruz.br/handle/icict/1292210.1186/s12879-015-0799-xengBioMed CentralApoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilãncia para Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilãncia para Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilãncia para Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilãncia para Leishmaniose. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Escola de Medicina. Departamento de Otorrinolaringologia e Oftalmologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação celular. Rio de Janeiro, RJ, Brasil /Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação celular. Rio de Janeiro, RJ, Brasil.Background: Leishmaniasis is an important parasitic disease affecting millions worldwide. Human cutaneous leishmaniasis (CL) is endemic in Rio de Janeiro, Brazil, where is caused by Leishmania braziliensis. The adaptive immune response is accountable for the healing of CL and despite of key role of CD8+ T cells in this immune response little is known about the CD8+ T lymphocytes frequencies, apoptosis and antigen-responsive CD8+ T lymphocytes of CL patients during antimonial therapy. Methods: Using flow cytometry, we examined total and effector CD8+ T cells from CL patients before (PBT), during (PDT) and after (PAT) treatment for apoptosis and frequencies upon isolation and after in vitro L. braziliensis antigens (LbAg)-stimulation culture. Besides, a correlation study between immunological findings and lesion size was done. Results: PDT showed lower frequencies of total CD8+ T lymphocytes and higher levels of apoptosis of these cells, which were also observed following LbAg-stimulation culture. Regarding effector CD8+ T cells, high frequencies were observed in PDT, while lower frequencies were observed in PAT. Interestingly, PDT showed higher frequencies of apoptotic-effector CD8+ T lymphocytes. Similar results were seen after in vitro antigenic-stimulation assays. Correlation analysis showed that the greater the size of lesion, the smaller the frequency of effector CD8+ T lymphocytes in PDT and PAT, as well as a positive correlation between apoptotic-effector CD8+ T cells frequency and lesion size of PDT. Conclusions: Changes in effector CD8+ T–lymphocyte frequencies, during and after treatment, seem to represent a critical stage to generate an efficient immune response and suggest that these cells would be evolved in the triggering or in the resolution of lesion, under the influence of therapy. This hypothesis opens new perspectives to clarify controversial statements about the protective or deleterious role of CD8+ T cells in the cure or aggravation of CL and the new approach of evaluating patients during treatment proved to be of utmost importance for understanding the immune response in the healing process of human CL.Flow cytometryEffector CD8+ T lymphocytesApoptosisHuman cutaneous leishmaniasisDuring antimonial treatmentLeishmania braziliensisCitometria de FluxoApoptoseLeishmania braziliensisLeishmaniose Cutâneainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| title |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| spellingShingle |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy Ferraz, Raquel Flow cytometry Effector CD8+ T lymphocytes Apoptosis Human cutaneous leishmaniasis During antimonial treatment Leishmania braziliensis Citometria de Fluxo Apoptose Leishmania braziliensis Leishmaniose Cutânea |
| title_short |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| title_full |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| title_fullStr |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| title_full_unstemmed |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| title_sort |
Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy |
| author |
Ferraz, Raquel |
| author_facet |
Ferraz, Raquel Cunha, Clarissa F. Silva, Adriano Gomes Schubach, Armando O. Pimentel, Maria Inês F. Lyra, Marcelo Rosandiski Mendonça, Sérgio C. F. Rosalino, Cláudia M. Valente Cruz, Alda Maria da Bertho, Álvaro Luiz |
| author_role |
author |
| author2 |
Cunha, Clarissa F. Silva, Adriano Gomes Schubach, Armando O. Pimentel, Maria Inês F. Lyra, Marcelo Rosandiski Mendonça, Sérgio C. F. Rosalino, Cláudia M. Valente Cruz, Alda Maria da Bertho, Álvaro Luiz |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferraz, Raquel Cunha, Clarissa F. Silva, Adriano Gomes Schubach, Armando O. Pimentel, Maria Inês F. Lyra, Marcelo Rosandiski Mendonça, Sérgio C. F. Rosalino, Cláudia M. Valente Cruz, Alda Maria da Bertho, Álvaro Luiz |
| dc.subject.en.pt_BR.fl_str_mv |
Flow cytometry Effector CD8+ T lymphocytes Apoptosis Human cutaneous leishmaniasis During antimonial treatment Leishmania braziliensis |
| topic |
Flow cytometry Effector CD8+ T lymphocytes Apoptosis Human cutaneous leishmaniasis During antimonial treatment Leishmania braziliensis Citometria de Fluxo Apoptose Leishmania braziliensis Leishmaniose Cutânea |
| dc.subject.decs.pt_BR.fl_str_mv |
Citometria de Fluxo Apoptose Leishmania braziliensis Leishmaniose Cutânea |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação celular. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015 |
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2016-03-01T15:29:07Z |
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2016-03-01T15:29:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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FERRAZ, Raquel; et al. Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy. BMC Infectious Diseases, v.15, n.74, 11p., 2015. |
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https://www.arca.fiocruz.br/handle/icict/12922 |
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1471-2334 |
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10.1186/s12879-015-0799-x |
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FERRAZ, Raquel; et al. Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy. BMC Infectious Diseases, v.15, n.74, 11p., 2015. 1471-2334 10.1186/s12879-015-0799-x |
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BioMed Central |
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BioMed Central |
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