Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses

Bibliographic Details
Main Author: Fernandes, Rafaela S.
Publication Date: 2020
Other Authors: Freire, Marjorie C. L. C., Bueno, Renata V., Godoy, Andre S., Gil, Laura H. V. G., Oliva, Glaucius
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/42004
Summary: Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
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spelling Fernandes, Rafaela S.Freire, Marjorie C. L. C.Bueno, Renata V.Godoy, Andre S.Gil, Laura H. V. G.Oliva, Glaucius2020-06-30T15:42:31Z2020-06-30T15:42:31Z2020FERNANDES, Rafaela S. et al. Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses. Viruses, v.12, n. 598, p. 1-22, 2020.1999-4915https://www.arca.fiocruz.br/handle/icict/4200410.3390/v12060598engMDPIReporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Virusesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific treatments are not available for most viral infections, the discovery of direct-acting antivirals (DAA) is an urgent need. Still, the low-throughput nature of and biosafety concerns related to traditional antiviral assays hinders the discovery of new inhibitors. With the advances of reverse genetics, reporter replicon systems have become an alternative tool for the screening of DAAs. Herein, we review decades of the use of (+) ssRNA viruses replicon systems for the discovery of antiviral agents. We summarize different strategies used to develop those systems, as well as highlight some of the most promising inhibitors identified by the method. Despite the genetic alterations introduced, reporter replicons have been shown to be reliable systems for screening and identification of viral replication inhibitors and, therefore, an important tool for the discovery of new DAAs.Replicon Systems(+) ssRNA virusesDirect-acting antiviralsDrug DiscoverySARS-CoV-2COVID-19Coronavirusinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83132https://www.arca.fiocruz.br/bitstream/icict/42004/1/license.txt70425d8cb6e40fdcf4edd2ef0660b45bMD51ORIGINALFernandes_Rafaela_etal_IAM_2020_COVID-19.pdfFernandes_Rafaela_etal_IAM_2020_COVID-19.pdfapplication/pdf1595283https://www.arca.fiocruz.br/bitstream/icict/42004/2/Fernandes_Rafaela_etal_IAM_2020_COVID-19.pdf9c6591aa0402cb91b935f21ae54cf93bMD52TEXTFernandes_Rafaela_etal_IAM_2020_COVID-19.pdf.txtFernandes_Rafaela_etal_IAM_2020_COVID-19.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
spellingShingle Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
Fernandes, Rafaela S.
Replicon Systems
(+) ssRNA viruses
Direct-acting antivirals
Drug Discovery
SARS-CoV-2
COVID-19
Coronavirus
title_short Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_full Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_fullStr Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_full_unstemmed Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_sort Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
author Fernandes, Rafaela S.
author_facet Fernandes, Rafaela S.
Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
author_role author
author2 Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Rafaela S.
Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
dc.subject.en.pt_BR.fl_str_mv Replicon Systems
(+) ssRNA viruses
Direct-acting antivirals
Drug Discovery
SARS-CoV-2
COVID-19
Coronavirus
topic Replicon Systems
(+) ssRNA viruses
Direct-acting antivirals
Drug Discovery
SARS-CoV-2
COVID-19
Coronavirus
description Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-06-30T15:42:31Z
dc.date.available.fl_str_mv 2020-06-30T15:42:31Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, Rafaela S. et al. Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses. Viruses, v.12, n. 598, p. 1-22, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42004
dc.identifier.issn.pt_BR.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v12060598
identifier_str_mv FERNANDES, Rafaela S. et al. Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses. Viruses, v.12, n. 598, p. 1-22, 2020.
1999-4915
10.3390/v12060598
url https://www.arca.fiocruz.br/handle/icict/42004
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/42004/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/42004/2/Fernandes_Rafaela_etal_IAM_2020_COVID-19.pdf
https://www.arca.fiocruz.br/bitstream/icict/42004/3/Fernandes_Rafaela_etal_IAM_2020_COVID-19.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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