De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17959 |
Resumo: | INCT/MCT/CNPq Programme – Contract no. 5737862008; MCTI/CNPq/FNDCT Ação Transversal N◦79/2013 (RENORBIO); FAPESB (PET0005/2010 and JCB017/2013); and CAPES (projectn◦077 2012). |
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Santos, Leonardo NascimentoSilva, Eduardo Santos daSantos, André SSá, Pablo Henrique Caracciolo Gomes deRamos, Rommel Thiago JucaSilva, Artur Luiz da Costa daCooper, Philip JBarreto, Maurício LimaLoureiro, SebastiãoPinheiro, Carina da SilvaNeves, Neuza Maria AlcântaraPacheco, Luis Gustavo Carvalho2017-03-07T14:08:55Z2017-03-07T14:08:55Z2016SANTOS, L. N. et al. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing. Acta Tropica, v. 159, p. 132–141, 2016.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/1795910.1016/j.actatropica.2016.03.036INCT/MCT/CNPq Programme – Contract no. 5737862008; MCTI/CNPq/FNDCT Ação Transversal N◦79/2013 (RENORBIO); FAPESB (PET0005/2010 and JCB017/2013); and CAPES (projectn◦077 2012).Federal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Pará. Institute of Biological Sciences. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Belém, PA, BrazilSt. George’s University of London. Institute of Infection and Immunity. London, UK / Pontificia Universidad Catolica del Ecuador. Centro de Investigacion en Enfermedades Infecciosas y Cronicas. Quito, EcuadorFederal University of Bahia. Institute of Public Health. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Institute of Public Health. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilFederal University of Bahia. Institute of Health Sciences. Salvador, BA, BrazilInfection with helminthic parasites, including the soil-transmitted helminth Trichuris trichiura (human whipworm), has been shown to modulate host immune responses and, consequently, to have an impact on the development and manifestation of chronic human inflammatory diseases. De novo derivation of helminth proteomes from sequencing of transcriptomes will provide valuable data to aid identification of parasite proteins that could be evaluated as potential immunotherapeutic molecules in near future. Herein, we characterized the transcriptome of the adult stage of the human whipworm T. trichiura, using next-generation sequencing technology and a de novo assembly strategy. Nearly 17.6 million high-quality clean reads were assembled into 6414 contiguous sequences, with an N50 of 1606bp. In total, 5673 protein-encoding sequences were confidentially identified in the T. trichiura adult worm transcriptome; of these, 1013 sequences represent potential newly discovered proteins for the species, most of which presenting orthologs already annotated in the related species T. suis. A number of transcripts representing probable novel non-coding transcripts for the species T. trichiura were also identified. Among the most abundant transcripts, we found sequences that code for proteins involved in lipid transport, such as vitellogenins, and several chitin-binding proteins. Through a cross-species expression analysis of gene orthologs shared by T. trichiura and the closely related parasites T. suis and T. muris it was possible to find twenty-six protein-encoding genes that are consistently highly expressed in the adult stages of the three helminth species. Additionally, twenty transcripts could be identified that code for proteins previously detected by mass spectrometry analysis of protein fractions of the whipworm somatic extract that present immunomodulatory activities. Five of these transcripts were amongst the most highly expressed protein-encoding sequences in the T. trichiura adult worm. Besides, orthologs of proteins demonstrated to have potent immunomodulatory properties in related parasitic helminths were also predicted from the T. trichiura de novo assembled transcriptome.engElsevierTrichuris trichiuraTranscriptomeSeqüenciamento da próxima geraçãoGenômica funcionalTrichuris trichiuraTranscriptomeNext-generation sequencingFunctional genomicsDe novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17959/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos LN De novo assembly....pdfSantos LN De novo assembly....pdfapplication/pdf2150313https://www.arca.fiocruz.br/bitstream/icict/17959/2/Santos%20LN%20De%20novo%20assembly....pdf443932249fb8e3856c3012e9ef1b85eaMD52TEXTSantos LN De novo assembly....pdf.txtSantos LN De novo assembly....pdf.txtExtracted texttext/plain56923https://www.arca.fiocruz.br/bitstream/icict/17959/3/Santos%20LN%20De%20novo%20assembly....pdf.txt8a5b922623ef5e4a1064b96bdb3fe455MD53icict/179592023-03-15 14:34:05.853oai:www.arca.fiocruz.br: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ório 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| dc.title.pt_BR.fl_str_mv |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| title |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| spellingShingle |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing Santos, Leonardo Nascimento Trichuris trichiura Transcriptome Seqüenciamento da próxima geração Genômica funcional Trichuris trichiura Transcriptome Next-generation sequencing Functional genomics |
| title_short |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| title_full |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| title_fullStr |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| title_full_unstemmed |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| title_sort |
De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing |
| author |
Santos, Leonardo Nascimento |
| author_facet |
Santos, Leonardo Nascimento Silva, Eduardo Santos da Santos, André S Sá, Pablo Henrique Caracciolo Gomes de Ramos, Rommel Thiago Juca Silva, Artur Luiz da Costa da Cooper, Philip J Barreto, Maurício Lima Loureiro, Sebastião Pinheiro, Carina da Silva Neves, Neuza Maria Alcântara Pacheco, Luis Gustavo Carvalho |
| author_role |
author |
| author2 |
Silva, Eduardo Santos da Santos, André S Sá, Pablo Henrique Caracciolo Gomes de Ramos, Rommel Thiago Juca Silva, Artur Luiz da Costa da Cooper, Philip J Barreto, Maurício Lima Loureiro, Sebastião Pinheiro, Carina da Silva Neves, Neuza Maria Alcântara Pacheco, Luis Gustavo Carvalho |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Leonardo Nascimento Silva, Eduardo Santos da Santos, André S Sá, Pablo Henrique Caracciolo Gomes de Ramos, Rommel Thiago Juca Silva, Artur Luiz da Costa da Cooper, Philip J Barreto, Maurício Lima Loureiro, Sebastião Pinheiro, Carina da Silva Neves, Neuza Maria Alcântara Pacheco, Luis Gustavo Carvalho |
| dc.subject.other.pt_BR.fl_str_mv |
Trichuris trichiura Transcriptome Seqüenciamento da próxima geração Genômica funcional |
| topic |
Trichuris trichiura Transcriptome Seqüenciamento da próxima geração Genômica funcional Trichuris trichiura Transcriptome Next-generation sequencing Functional genomics |
| dc.subject.en.pt_BR.fl_str_mv |
Trichuris trichiura Transcriptome Next-generation sequencing Functional genomics |
| description |
INCT/MCT/CNPq Programme – Contract no. 5737862008; MCTI/CNPq/FNDCT Ação Transversal N◦79/2013 (RENORBIO); FAPESB (PET0005/2010 and JCB017/2013); and CAPES (projectn◦077 2012). |
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2016 |
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2016 |
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2017-03-07T14:08:55Z |
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2017-03-07T14:08:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SANTOS, L. N. et al. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing. Acta Tropica, v. 159, p. 132–141, 2016. |
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https://www.arca.fiocruz.br/handle/icict/17959 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0001-706X |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2016.03.036 |
| identifier_str_mv |
SANTOS, L. N. et al. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing. Acta Tropica, v. 159, p. 132–141, 2016. 0001-706X 10.1016/j.actatropica.2016.03.036 |
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Elsevier |
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Elsevier |
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