Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50425 |
Resumo: | Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico – CNPq (grant no. 400905/2013-2) and the research productivity scholarship provided to WS. We are also thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB (no. PET0053_2013). |
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Melo, Caroline Vilas Boas deTorres, Felipe GuimarãesHermida, Micely D’El-ReiFontes, Jonathan L. M.Mesquita, Bianca RamosBrito, ReginaldoRamos, Pablo Ivan P.Fernandes, Gabriel R.Freitas, Luiz Antônio RodriguesKhouri, Antonio RicardoCosta, Carlos Henrique Nerydos-Santos, Washington Luis Conrado2021-12-14T18:22:02Z2021-12-14T18:22:02Z2021MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/5042510.3389/fimmu.2021.716314Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico – CNPq (grant no. 400905/2013-2) and the research productivity scholarship provided to WS. We are also thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB (no. PET0053_2013).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Enfermidades Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Centro de Integração de Dados e Conhecimento para Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Rene´ Rachou. Informática de Biossistemas. Minas Gerais, BH, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Enfermidades Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Universidade Federal do Piauí. Instituto de Doenças Tropicais Natan Portella. Departamento de Medicina Comunitária. Teresina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Structural changes in the spleen have been reported in several infectious diseases. In visceral leishmaniasis (VL), a severe parasitic disease caused by Leishmania spp., the loss of white pulp accompanies a severe clinical presentation. Hamster model reproduces aspects of human VL progression. In the early stages, a transcriptomic signature of leukocyte recruitment was associated with white pulp hyperplasia. Subsequently, impaired leukocyte chemotaxis with loss of T lymphocytes in the periarteriolar lymphoid sheath occurred. This differential gene expression was subsequently corroborated by transcriptomic profiling of spleens in severe human VL. At the latest stage, spleen disorganization was associated with increasing clinical signs of VL. White pulp disruption was accompanied by decreased DLK1 expression. The expression of CXCL13, CCR5, CCL19, CCR6, CCR7 and LTA decreased, likely regulated by CDKN2A overexpression. Our findings enlighten a pathway implying cell cycle arrest and decreased gene expression involved in spleen organization.engFrontiers MediaLeishmaniose visceralLeucócitosBaçoRNA-SeqBaço patologiaHiperplasiaVisceral leishmanaisisWhite pulp remodelingSpleen disorganizationTranscriptomic (RNA-Seq)Spleen pathology, hamsterSplenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrestinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/50425/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALMelo, Caroline Vilas Boas Splenic....pdfMelo, Caroline Vilas Boas Splenic....pdfapplication/pdf39628867https://www.arca.fiocruz.br/bitstream/icict/50425/2/Melo%2c%20Caroline%20Vilas%20Boas%20Splenic....pdf42e58e3596211ff13577c208f37968baMD52icict/504252023-03-15 14:34:32.564oai:www.arca.fiocruz.br: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Repositório 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| dc.title.pt_BR.fl_str_mv |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| title |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| spellingShingle |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest Melo, Caroline Vilas Boas de Leishmaniose visceral Leucócitos Baço RNA-Seq Baço patologia Hiperplasia Visceral leishmanaisis White pulp remodeling Spleen disorganization Transcriptomic (RNA-Seq) Spleen pathology, hamster |
| title_short |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| title_full |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| title_fullStr |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| title_full_unstemmed |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| title_sort |
Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest |
| author |
Melo, Caroline Vilas Boas de |
| author_facet |
Melo, Caroline Vilas Boas de Torres, Felipe Guimarães Hermida, Micely D’El-Rei Fontes, Jonathan L. M. Mesquita, Bianca Ramos Brito, Reginaldo Ramos, Pablo Ivan P. Fernandes, Gabriel R. Freitas, Luiz Antônio Rodrigues Khouri, Antonio Ricardo Costa, Carlos Henrique Nery dos-Santos, Washington Luis Conrado |
| author_role |
author |
| author2 |
Torres, Felipe Guimarães Hermida, Micely D’El-Rei Fontes, Jonathan L. M. Mesquita, Bianca Ramos Brito, Reginaldo Ramos, Pablo Ivan P. Fernandes, Gabriel R. Freitas, Luiz Antônio Rodrigues Khouri, Antonio Ricardo Costa, Carlos Henrique Nery dos-Santos, Washington Luis Conrado |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Melo, Caroline Vilas Boas de Torres, Felipe Guimarães Hermida, Micely D’El-Rei Fontes, Jonathan L. M. Mesquita, Bianca Ramos Brito, Reginaldo Ramos, Pablo Ivan P. Fernandes, Gabriel R. Freitas, Luiz Antônio Rodrigues Khouri, Antonio Ricardo Costa, Carlos Henrique Nery dos-Santos, Washington Luis Conrado |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose visceral Leucócitos Baço RNA-Seq Baço patologia Hiperplasia |
| topic |
Leishmaniose visceral Leucócitos Baço RNA-Seq Baço patologia Hiperplasia Visceral leishmanaisis White pulp remodeling Spleen disorganization Transcriptomic (RNA-Seq) Spleen pathology, hamster |
| dc.subject.en.pt_BR.fl_str_mv |
Visceral leishmanaisis White pulp remodeling Spleen disorganization Transcriptomic (RNA-Seq) Spleen pathology, hamster |
| description |
Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico – CNPq (grant no. 400905/2013-2) and the research productivity scholarship provided to WS. We are also thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB (no. PET0053_2013). |
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2021 |
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2021-12-14T18:22:02Z |
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2021-12-14T18:22:02Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021. |
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https://www.arca.fiocruz.br/handle/icict/50425 |
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1664-3224 |
| dc.identifier.eissn.none.fl_str_mv |
10.3389/fimmu.2021.716314 |
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MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021. 1664-3224 10.3389/fimmu.2021.716314 |
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