Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45739 |
Resumo: | Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil. |
| id |
CRUZ_3520eee68e37f17e882d6fddbe7a37c3 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/45739 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Silva, Marcelo S. daCayres-Silva, Gustavo R.Vitarelli, Marcela O.Marin, Paula A.Hiraiwa, Priscila MazzocchiAraújo, Christiane B.Scholl, Bruno B.Ávila, Andréa RodriguesMcCulloch, RichardReis, Marcelo S.Elias, Maria Carolina2021-01-19T17:22:41Z2021-01-19T17:22:41Z2020SILVA, Marcelo S. da et al. Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration. PLoS ONE, v.9, n. 18512, p. 1–19, 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/45739doi.org/10.1038/s41598-019-54366-wporNature ResearchTranscription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase durationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Citometria de Fluxo. Curitiba, PR, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil.The co-synthesis of DNA and RNA potentially generates conficts between replication and transcription, which can lead to genomic instability. In trypanosomatids, eukaryotic parasites that perform polycistronic transcription, this phenomenon and its consequences are still little studied. Here, we showed that the number of constitutive origins mapped in the Trypanosoma brucei genome is less than the minimum required to complete replication within S-phase duration. By the development of a mechanistic model of DNA replication considering replication-transcription conficts and using immunofuorescence assays and DNA combing approaches, we demonstrated that the activation of non-constitutive (backup) origins are indispensable for replication to be completed within S-phase period. Together, our fndings suggest that transcription activity during S phase generates R-loops, which contributes to the emergence of DNA lesions, leading to the fring of backup origins that help maintain robustness in S-phase duration. The usage of this increased pool of origins, contributing to the maintenance of DNA replication, seems to be of paramount importance for the survival of this parasite that afects million people around the world.DNA ReplicationTranscription, GeneticS PhaseReplicación del ADNTranscripción GenéticaRéplication de l'ADNTranscription génétiquePhase SReplicação do DNATrypanosomaTranscrição GenéticaTrypanosoma bruceiFase Sinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/45739/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-019-54366OK.pdfs41598-019-54366OK.pdfapplication/pdf6236893https://www.arca.fiocruz.br/bitstream/icict/45739/2/s41598-019-54366OK.pdfe8d7fed9bcef615064629f89d9e5f1d6MD52TEXTs41598-019-54366OK.pdf.txts41598-019-54366OK.pdf.txtExtracted texttext/plain103413https://www.arca.fiocruz.br/bitstream/icict/45739/3/s41598-019-54366OK.pdf.txtc11e42a28df6bc10aa1021ffab8b2620MD53icict/457392021-01-20 02:13:52.104oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-01-20T05:13:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| title |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| spellingShingle |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration Silva, Marcelo S. da DNA Replication Transcription, Genetic S Phase Replicación del ADN Transcripción Genética Réplication de l'ADN Transcription génétique Phase S Replicação do DNA Trypanosoma Transcrição Genética Trypanosoma brucei Fase S |
| title_short |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| title_full |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| title_fullStr |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| title_full_unstemmed |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| title_sort |
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration |
| author |
Silva, Marcelo S. da |
| author_facet |
Silva, Marcelo S. da Cayres-Silva, Gustavo R. Vitarelli, Marcela O. Marin, Paula A. Hiraiwa, Priscila Mazzocchi Araújo, Christiane B. Scholl, Bruno B. Ávila, Andréa Rodrigues McCulloch, Richard Reis, Marcelo S. Elias, Maria Carolina |
| author_role |
author |
| author2 |
Cayres-Silva, Gustavo R. Vitarelli, Marcela O. Marin, Paula A. Hiraiwa, Priscila Mazzocchi Araújo, Christiane B. Scholl, Bruno B. Ávila, Andréa Rodrigues McCulloch, Richard Reis, Marcelo S. Elias, Maria Carolina |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Marcelo S. da Cayres-Silva, Gustavo R. Vitarelli, Marcela O. Marin, Paula A. Hiraiwa, Priscila Mazzocchi Araújo, Christiane B. Scholl, Bruno B. Ávila, Andréa Rodrigues McCulloch, Richard Reis, Marcelo S. Elias, Maria Carolina |
| dc.subject.en.pt_BR.fl_str_mv |
DNA Replication Transcription, Genetic S Phase |
| topic |
DNA Replication Transcription, Genetic S Phase Replicación del ADN Transcripción Genética Réplication de l'ADN Transcription génétique Phase S Replicação do DNA Trypanosoma Transcrição Genética Trypanosoma brucei Fase S |
| dc.subject.es.pt_BR.fl_str_mv |
Replicación del ADN Transcripción Genética |
| dc.subject.fr.pt_BR.fl_str_mv |
Réplication de l'ADN Transcription génétique Phase S |
| dc.subject.decs.pt_BR.fl_str_mv |
Replicação do DNA Trypanosoma Transcrição Genética Trypanosoma brucei Fase S |
| description |
Instituto Butantan. Laboratório Especial de Ciclo Celular. Butantã, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil. |
| publishDate |
2020 |
| dc.date.issued.fl_str_mv |
2020 |
| dc.date.accessioned.fl_str_mv |
2021-01-19T17:22:41Z |
| dc.date.available.fl_str_mv |
2021-01-19T17:22:41Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
SILVA, Marcelo S. da et al. Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration. PLoS ONE, v.9, n. 18512, p. 1–19, 2019. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/45739 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
doi.org/10.1038/s41598-019-54366-w |
| identifier_str_mv |
SILVA, Marcelo S. da et al. Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration. PLoS ONE, v.9, n. 18512, p. 1–19, 2019. 2045-2322 doi.org/10.1038/s41598-019-54366-w |
| url |
https://www.arca.fiocruz.br/handle/icict/45739 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Nature Research |
| publisher.none.fl_str_mv |
Nature Research |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/45739/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/45739/2/s41598-019-54366OK.pdf https://www.arca.fiocruz.br/bitstream/icict/45739/3/s41598-019-54366OK.pdf.txt |
| bitstream.checksum.fl_str_mv |
783568c2893d2e25a99990b126be1772 e8d7fed9bcef615064629f89d9e5f1d6 c11e42a28df6bc10aa1021ffab8b2620 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324994984378368 |