Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20045 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. |
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Cavados, Clara de Fátima GomesPires, Eder SoaresChaves, Jeane QuintanilhaAlvarez, Danielle NunesGil, Helio BenitesOliveira, Iris Braz Ribeiro dePinto, Andrea de BarrosCunha, VivianiCoutinho, Carlos José Pereira da Cunha de Araújo2017-07-11T17:21:42Z2017-07-11T17:21:42Z2017CAVADOS, Clara de Fátima Gomes; et al. Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae). Research and Reports in Tropical Medicine, v.8, p.17-20, 2017.1179-7282https://www.arca.fiocruz.br/handle/icict/2004510.2147/RRTM.S124066engDove Medical PressCulicidaeMosquitosBioensaios qualitativosBactéria entomopatogênicaReação em Cadeia da Polimerase em Tempo RealLysinibacillus sphaericusLysinibacillus sphaericusCulicidaequalitative bioassaysREP-PCRmosquitoesentomopathogenic bacteriaIsolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil.Superintendência de Controle de Endemias. São Paulo, SP, Brasil.Superintendência de Controle de Endemias. São Paulo, SP, Brasil.Superintendência de Controle de Endemias. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil.Introduction: Lysinibacillus sphaericus is a highly effective and specific bioinsecticide used for the control of Culicidae larvae. Objective: This study aimed to identify and characterize L. sphaericus strains isolated from Culex quinquefasciatus larvae in Brazil. Methods: C. quinquefasciatus larvae were collected from streams in the urban area of São Paulo state. L. sphaericus strains were identified through cytomorphology, biochemical, and physiological analyses. Qualitative bioassays were performed to evaluate the toxicity of the strains against C. quinquefasciatus. The crystal compound protein pattern of L. sphaericus strains was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Five reference strains were used as standards in all tests performed. Repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) was utilized in an attempt to differentiate pathogenic and nonpathogenic isolates. Results: Twenty-one strains were isolated. Only one presented toxic activity against C. quinquefasciatus. REP-PCR results identified 23 patterns among the 26 strains used in the study, and the fragment analysis showed low similarity (16%) between L. sphaericus isolates and the five reference strains. Conclusion: Comparison of strains isolated in this study using REP-PCR showed a low similarity to other strains, demonstrating the high intraspecific variability for L. sphaericus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20045/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALclara_cavados_etal_IOC_2017.pdfclara_cavados_etal_IOC_2017.pdfapplication/pdf170348https://www.arca.fiocruz.br/bitstream/icict/20045/2/clara_cavados_etal_IOC_2017.pdf02fddb509e540692eb967ca6f4977085MD52TEXTclara_cavados_etal_IOC_2017.pdf.txtclara_cavados_etal_IOC_2017.pdf.txtExtracted texttext/plain20235https://www.arca.fiocruz.br/bitstream/icict/20045/3/clara_cavados_etal_IOC_2017.pdf.txt054f1c54030ad61713d52d3475d6983dMD53icict/200452018-08-15 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| dc.title.pt_BR.fl_str_mv |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| title |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| spellingShingle |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) Cavados, Clara de Fátima Gomes Culicidae Mosquitos Bioensaios qualitativos Bactéria entomopatogênica Reação em Cadeia da Polimerase em Tempo Real Lysinibacillus sphaericus Lysinibacillus sphaericus Culicidae qualitative bioassays REP-PCR mosquitoes entomopathogenic bacteria |
| title_short |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| title_full |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| title_fullStr |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| title_full_unstemmed |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| title_sort |
Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae) |
| author |
Cavados, Clara de Fátima Gomes |
| author_facet |
Cavados, Clara de Fátima Gomes Pires, Eder Soares Chaves, Jeane Quintanilha Alvarez, Danielle Nunes Gil, Helio Benites Oliveira, Iris Braz Ribeiro de Pinto, Andrea de Barros Cunha, Viviani Coutinho, Carlos José Pereira da Cunha de Araújo |
| author_role |
author |
| author2 |
Pires, Eder Soares Chaves, Jeane Quintanilha Alvarez, Danielle Nunes Gil, Helio Benites Oliveira, Iris Braz Ribeiro de Pinto, Andrea de Barros Cunha, Viviani Coutinho, Carlos José Pereira da Cunha de Araújo |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cavados, Clara de Fátima Gomes Pires, Eder Soares Chaves, Jeane Quintanilha Alvarez, Danielle Nunes Gil, Helio Benites Oliveira, Iris Braz Ribeiro de Pinto, Andrea de Barros Cunha, Viviani Coutinho, Carlos José Pereira da Cunha de Araújo |
| dc.subject.other.pt_BR.fl_str_mv |
Culicidae Mosquitos Bioensaios qualitativos Bactéria entomopatogênica Reação em Cadeia da Polimerase em Tempo Real Lysinibacillus sphaericus |
| topic |
Culicidae Mosquitos Bioensaios qualitativos Bactéria entomopatogênica Reação em Cadeia da Polimerase em Tempo Real Lysinibacillus sphaericus Lysinibacillus sphaericus Culicidae qualitative bioassays REP-PCR mosquitoes entomopathogenic bacteria |
| dc.subject.en.pt_BR.fl_str_mv |
Lysinibacillus sphaericus Culicidae qualitative bioassays REP-PCR mosquitoes entomopathogenic bacteria |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. |
| publishDate |
2017 |
| dc.date.accessioned.fl_str_mv |
2017-07-11T17:21:42Z |
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2017-07-11T17:21:42Z |
| dc.date.issued.fl_str_mv |
2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
CAVADOS, Clara de Fátima Gomes; et al. Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae). Research and Reports in Tropical Medicine, v.8, p.17-20, 2017. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/20045 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1179-7282 |
| dc.identifier.doi.none.fl_str_mv |
10.2147/RRTM.S124066 |
| identifier_str_mv |
CAVADOS, Clara de Fátima Gomes; et al. Isolation and genetic characterization of Lysinibacillus sphaericus strains found in mosquito larvae (Diptera: Culicidae). Research and Reports in Tropical Medicine, v.8, p.17-20, 2017. 1179-7282 10.2147/RRTM.S124066 |
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https://www.arca.fiocruz.br/handle/icict/20045 |
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eng |
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Dove Medical Press |
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Dove Medical Press |
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