A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil

Detalhes bibliográficos
Autor(a) principal: Mesquita, Silvia Gonçalves
Data de Publicação: 2021
Outros Autores: Neves, Floria Gabriela Dos Santos, Scholte, Ronaldo Guilherme Carvalho, Carvalho, Omar Dos Santos, Fonseca, Cristina Toscano, Caldeira, Roberta Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51438
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.
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spelling Mesquita, Silvia GonçalvesNeves, Floria Gabriela Dos SantosScholte, Ronaldo Guilherme CarvalhoCarvalho, Omar Dos SantosFonseca, Cristina ToscanoCaldeira, Roberta Lima2022-02-23T18:28:25Z2022-02-23T18:28:25Z2021MESQUITA, Silvia Gonçalves et al. A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil. Parasit Vectors, v. 14, n. 1, 388, 2021.doi: 10.1186/s13071-021-04888-y.1756-3305https://www.arca.fiocruz.br/handle/icict/5143810.1186/s13071-021-04888-yengBioMed CentralA loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia e Imunologia Parasitária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.Background: Schistosomiasis a neglected tropical disease endemic in Brazil. It is caused by the trematode Schistosoma mansoni, which is transmitted by snails of the genus Biomphalaria. Among measures used to control and eliminate schistosomiasis, accurate mapping and monitoring of snail breeding sites are recommended. Despite the limitations of parasitological methods, they are still used to identify infected snails. Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid, and cost-effective diagnostic method for the identification of infected snails. In the work reported here, we aimed to validate the use of LAMP for the detection of S. mansoni in snails of the genus Biomphalaria. Methods: Snails were collected in five municipalities of the Mucuri Valley and Jequitinhonha Valley regions in the state of Minas Gerais, Brazil. Snails were pooled according to collection site and then squeezed for the detection of S. mansoni and other trematode larvae. Pooled snails were subjected to pepsin digestion and DNA extraction. Molecular assays were performed for species-specific identification and characterization of the samples. A previously described LAMP assay was adapted, evaluated, and validated using laboratory and field samples. Results: Using the parasitological method described here, S. mansoni cercariae were detected in snails from two collection sites, and cercariae of the family Spirorchiidae were found in snails from one site. The snails were identified by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Biomphalaria glabrata, the main snail host of S. mansoni in Brazil, was detected in 72.2% of the collection sites. Biomphalaria kuhniana, which is resistant to S. mansoni infection, was found in the remaining sites. Multiplex, low stringency (LS), and conventional PCR allowed the detection of positive snails in four additional sites. Trematodes belonging to the families Strigeidae and Echinostomatidae were detected by multiplex PCR in two sites. The LAMP assay was effective in detecting the presence of S. mansoni infection in laboratory (7 days post-infection) and field samples with no cross-reactivity for other trematodes. When compared to LS and conventional PCR, LAMP showed 100% specificity, 85.7% sensitivity, and a κ index of 0.88. Conclusions: Our findings suggest that LAMP is a good alternative method for the detection and monitoring of transmission foci of S. mansoni, as it was three times as effective as the parasitological examination used here for the detection of infection, and is more directly applicable in the field than other molecular techniques.BiomphalariaLAMPMolecular diagnosticsSchistosoma mansoniSchistosomiasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51438/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALA loop-mediated isothermal amplification assay for Schistosoma mansoni .pdfA loop-mediated isothermal amplification assay for Schistosoma mansoni 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dc.title.pt_BR.fl_str_mv A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
title A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
spellingShingle A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
Mesquita, Silvia Gonçalves
Biomphalaria
LAMP
Molecular diagnostics
Schistosoma mansoni
Schistosomiasis
title_short A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
title_full A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
title_fullStr A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
title_full_unstemmed A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
title_sort A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil
author Mesquita, Silvia Gonçalves
author_facet Mesquita, Silvia Gonçalves
Neves, Floria Gabriela Dos Santos
Scholte, Ronaldo Guilherme Carvalho
Carvalho, Omar Dos Santos
Fonseca, Cristina Toscano
Caldeira, Roberta Lima
author_role author
author2 Neves, Floria Gabriela Dos Santos
Scholte, Ronaldo Guilherme Carvalho
Carvalho, Omar Dos Santos
Fonseca, Cristina Toscano
Caldeira, Roberta Lima
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mesquita, Silvia Gonçalves
Neves, Floria Gabriela Dos Santos
Scholte, Ronaldo Guilherme Carvalho
Carvalho, Omar Dos Santos
Fonseca, Cristina Toscano
Caldeira, Roberta Lima
dc.subject.en.pt_BR.fl_str_mv Biomphalaria
LAMP
Molecular diagnostics
Schistosoma mansoni
Schistosomiasis
topic Biomphalaria
LAMP
Molecular diagnostics
Schistosoma mansoni
Schistosomiasis
description Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-23T18:28:25Z
dc.date.available.fl_str_mv 2022-02-23T18:28:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MESQUITA, Silvia Gonçalves et al. A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil. Parasit Vectors, v. 14, n. 1, 388, 2021.doi: 10.1186/s13071-021-04888-y.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51438
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-021-04888-y
identifier_str_mv MESQUITA, Silvia Gonçalves et al. A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil. Parasit Vectors, v. 14, n. 1, 388, 2021.doi: 10.1186/s13071-021-04888-y.
1756-3305
10.1186/s13071-021-04888-y
url https://www.arca.fiocruz.br/handle/icict/51438
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51438/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51438/2/A%20loop-mediated%20isothermal%20amplification%20assay%20for%20Schistosoma%20mansoni%20.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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