Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector

Detalhes bibliográficos
Autor(a) principal: Simone, Salvatore Giovanni de
Data de Publicação: 1987
Outros Autores: Pontes-de-Carvalho, Lain Carlos, Oliva, Otavio F. P, Andrade, Sonia Gumes, Castro Filho, Bernardo Galvão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8343
Resumo: Fundação Oswaldo Cruz. Bio-Manguinhos. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
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spelling Simone, Salvatore Giovanni dePontes-de-Carvalho, Lain CarlosOliva, Otavio F. PAndrade, Sonia GumesCastro Filho, Bernardo Galvão2014-09-10T17:01:17Z2014-09-10T17:01:17Z1987SIMONE, S. G. et al. Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 81, n. 5, p. 750-754, 1987.0035-9203https://www.arca.fiocruz.br/handle/icict/8343engRoyal Society of Tropical Medicine and HygieneTrypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vectorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Bio-Manguinhos. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Bio-Manguinhos. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil /Fundação Oswaldo Cruz. Bio-Manguinhos. Institute of Immunological Reagents. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Bio-Manguinhos. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilSpleen cells from mice immunized with insect-derived Typanosoma crUm metacyclic trypomastigotes were used to obtain Colombian strain-specific monoclonal antibodies. At least 4 different strain-specific antigens were recognized by the monoclonal antibodies on epimastigotes or metacyclic trypomastigotes. There was no reactivity with other stages of Colombian strain T. cruzi, nor with any stage of 15 other T. cruzi strains or isolates, nor with 22 other Trypanosomatidae. One of the monoclonal antibodies was used to identify, by indirect immunofluorescence, Colombian strain flagellates in cryostat sections or glass-slide smears of the insect vector’s intestine.Anticorpos Monoclonais/uso diagnósticoAnticorpos Antiprotozoários/análiseTrypanosoma cruzi/imunologiaAnimaisAnticorpos Antiprotozoários/imunologiaImunofluorescênciaInsetos Vetores/imunologiaInsetos Vetores/parasitologiaTriatominae/imunologiaTripanossomíase/parasitologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8343/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSimone SG Trypanosoma....pdfSimone SG Trypanosoma....pdfapplication/pdf3210797https://www.arca.fiocruz.br/bitstream/icict/8343/2/Simone%20SG%20Trypanosoma....pdfade73ad1f8f11cb84e41aacf0809d512MD52TEXTSimone SG Trypanosoma....pdf.txtSimone SG Trypanosoma....pdf.txtExtracted texttext/plain22528https://www.arca.fiocruz.br/bitstream/icict/8343/3/Simone%20SG%20Trypanosoma....pdf.txtf23b749247e8d67195a52041164b6157MD53icict/83432023-03-15 14:33:19.526oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
title Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
spellingShingle Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
Simone, Salvatore Giovanni de
Anticorpos Monoclonais/uso diagnóstico
Anticorpos Antiprotozoários/análise
Trypanosoma cruzi/imunologia
Animais
Anticorpos Antiprotozoários/imunologia
Imunofluorescência
Insetos Vetores/imunologia
Insetos Vetores/parasitologia
Triatominae/imunologia
Tripanossomíase/parasitologia
title_short Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
title_full Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
title_fullStr Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
title_full_unstemmed Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
title_sort Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector
author Simone, Salvatore Giovanni de
author_facet Simone, Salvatore Giovanni de
Pontes-de-Carvalho, Lain Carlos
Oliva, Otavio F. P
Andrade, Sonia Gumes
Castro Filho, Bernardo Galvão
author_role author
author2 Pontes-de-Carvalho, Lain Carlos
Oliva, Otavio F. P
Andrade, Sonia Gumes
Castro Filho, Bernardo Galvão
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Simone, Salvatore Giovanni de
Pontes-de-Carvalho, Lain Carlos
Oliva, Otavio F. P
Andrade, Sonia Gumes
Castro Filho, Bernardo Galvão
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Monoclonais/uso diagnóstico
Anticorpos Antiprotozoários/análise
Trypanosoma cruzi/imunologia
Animais
Anticorpos Antiprotozoários/imunologia
Imunofluorescência
Insetos Vetores/imunologia
Insetos Vetores/parasitologia
Triatominae/imunologia
Tripanossomíase/parasitologia
topic Anticorpos Monoclonais/uso diagnóstico
Anticorpos Antiprotozoários/análise
Trypanosoma cruzi/imunologia
Animais
Anticorpos Antiprotozoários/imunologia
Imunofluorescência
Insetos Vetores/imunologia
Insetos Vetores/parasitologia
Triatominae/imunologia
Tripanossomíase/parasitologia
description Fundação Oswaldo Cruz. Bio-Manguinhos. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
publishDate 1987
dc.date.issued.fl_str_mv 1987
dc.date.accessioned.fl_str_mv 2014-09-10T17:01:17Z
dc.date.available.fl_str_mv 2014-09-10T17:01:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SIMONE, S. G. et al. Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 81, n. 5, p. 750-754, 1987.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8343
dc.identifier.issn.none.fl_str_mv 0035-9203
identifier_str_mv SIMONE, S. G. et al. Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 81, n. 5, p. 750-754, 1987.
0035-9203
url https://www.arca.fiocruz.br/handle/icict/8343
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv Royal Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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