Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation

Detalhes bibliográficos
Autor(a) principal: Possidonio, Ana C. B.
Data de Publicação: 2014
Outros Autores: Miranda, Milene, Gregoracci, Gustavo B., Thompson, Fabiano L., Costa, Manoel L., Mermelstein, Claudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10829
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.
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spelling Possidonio, Ana C. B.Miranda, MileneGregoracci, Gustavo B.Thompson, Fabiano L.Costa, Manoel L.Mermelstein, Claudia2015-06-12T13:57:54Z2015-06-12T13:57:54Z2014POSSIDONIO, Ana C. B. et al. Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation. BMC Genomics, v.15:544, 9p., 2014.1471-2164https://www.arca.fiocruz.br/handle/icict/1082910.1186/1471-2164-15-544engBioMed CentralCholesterol depletion induces transcriptional changes during skeletal muscle differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.UNIFESP Baixada Santista. Departamento de Ciências do Mar. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Rio de Janeiro, RJ, Brasil.niversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.niversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Background: Myoblasts undergo major changes in their plasma membrane during the initial steps of skeletal muscle differentiation, including major alterations in the distribution of cholesterol. Cholesterol is involved in crucial membrane functions, such as fluidity, and permeability, and in the organization of specialized membrane microdomains (or lipid rafts). We have previously shown that alterations in cholesterol levels in myoblasts induce changes in proliferation and differentiation, which involves activation of Wnt/beta-catenin signaling pathway. In this study we used methyl-β-cyclodextrin (MbCD) to extract cholesterol from the membrane of chick skeletal muscle cells grown in culture. Using Ion Torrent-based sequencing, we compared the transcriptome of untreated and MbCD treated cells. Our aim was to define the genes that are expressed in these two conditions and relate their expression to cellular functions. Results: Over 5.7 million sequences were obtained, representing 671.38 Mb of information. mRNA transcriptome profiling of myogenic cells after cholesterol depletion revealed alterations in transcripts involved in the regulation of apoptosis, focal adhesion, phagosome, tight junction, cell cycle, lysosome, adherens junctions, gap junctions, p53 signaling pathway, endocytosis, autophagy and actin cytoskeleton. Lim domain only protein 7 mRNA was found to be the highest up-regulated feature after cholesterol depletion. Conclusions: This is the first study on the effects of membrane cholesterol depletion in mRNA expression in myogenic cells. Our data shows that alterations in the availability of plasma membrane cholesterol lead to transcriptional changes in myogenic cells. The knowledge of the genes involved in the cellular response to cholesterol depletion could contribute to our understanding of skeletal muscle differentiation.CholesterolSkeletal muscleMyoblastsMyogenic cellsColesterolMúsculo EsqueléticoMioblastosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10829/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmilene_mirandaetal_IOC_2014.pdfapplication/pdf3113546https://www.arca.fiocruz.br/bitstream/icict/10829/2/milene_mirandaetal_IOC_2014.pdf7a136ba41f13737570f316c6448b5638MD52TEXTmilene_mirandaetal_IOC_2014.pdf.txtmilene_mirandaetal_IOC_2014.pdf.txtExtracted texttext/plain39128https://www.arca.fiocruz.br/bitstream/icict/10829/3/milene_mirandaetal_IOC_2014.pdf.txt6e990cfd02be4255a552f355a6e10f7dMD53icict/108292022-06-24 13:09:04.41oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:09:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
title Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
spellingShingle Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
Possidonio, Ana C. B.
Cholesterol
Skeletal muscle
Myoblasts
Myogenic cells
Colesterol
Músculo Esquelético
Mioblastos
title_short Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
title_full Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
title_fullStr Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
title_full_unstemmed Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
title_sort Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation
author Possidonio, Ana C. B.
author_facet Possidonio, Ana C. B.
Miranda, Milene
Gregoracci, Gustavo B.
Thompson, Fabiano L.
Costa, Manoel L.
Mermelstein, Claudia
author_role author
author2 Miranda, Milene
Gregoracci, Gustavo B.
Thompson, Fabiano L.
Costa, Manoel L.
Mermelstein, Claudia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Possidonio, Ana C. B.
Miranda, Milene
Gregoracci, Gustavo B.
Thompson, Fabiano L.
Costa, Manoel L.
Mermelstein, Claudia
dc.subject.en.pt_BR.fl_str_mv Cholesterol
Skeletal muscle
Myoblasts
Myogenic cells
topic Cholesterol
Skeletal muscle
Myoblasts
Myogenic cells
Colesterol
Músculo Esquelético
Mioblastos
dc.subject.decs.pt_BR.fl_str_mv Colesterol
Músculo Esquelético
Mioblastos
description Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-12T13:57:54Z
dc.date.available.fl_str_mv 2015-06-12T13:57:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv POSSIDONIO, Ana C. B. et al. Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation. BMC Genomics, v.15:544, 9p., 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10829
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/1471-2164-15-544
identifier_str_mv POSSIDONIO, Ana C. B. et al. Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation. BMC Genomics, v.15:544, 9p., 2014.
1471-2164
10.1186/1471-2164-15-544
url https://www.arca.fiocruz.br/handle/icict/10829
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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