A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole

Zingales, Bianca; Araujo, Rafael Gomes Aquino; Franco, Jaques; Aguiar, Pedro Henrique Nascimento; Nunes, Solange Lessa; Silva, Marcelo Nunes; Ienne, Susan; Machado, Carlos Renato; Brandão, Adeilton; Moreno, Margoth

A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole

Detalhes bibliográficos
Autor(a) principal:	Zingales, Bianca
Data de Publicação:	2015
Outros Autores:	Araujo, Rafael Gomes Aquino, Franco, Jaques, Aguiar, Pedro Henrique Nascimento, Nunes, Solange Lessa, Silva, Marcelo Nunes, Ienne, Susan, Machado, Carlos Renato, Brandão, Adeilton, Moreno, Margoth
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/12234
Resumo:	Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.

Metadados do item

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spelling	Zingales, BiancaAraujo, Rafael Gomes AquinoFranco, JaquesAguiar, Pedro Henrique NascimentoNunes, Solange LessaSilva, Marcelo NunesIenne, SusanMachado, Carlos RenatoBrandão, AdeiltonMoreno, Margoth2015-11-16T12:12:42Z2015-11-16T12:12:42Z2015ZINGALES, Bianca; et al. A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole. Mem Inst Oswaldo Cruz, v.110, n.3, p. 433-444, May 2015.0074-0276https://www.arca.fiocruz.br/handle/icict/1223410.1590/0074-027601404071678-8060engFundação Oswaldo CruzA novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazoleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departtamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departtamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Benznidazole (BZ) is one of the two drugs used for Chagas disease treatment. Nevertheless therapeutic failures of BZ have been reported, which were mostly attributed to variable drug susceptibility among Trypanosoma cruzi strains. ATP-binding cassette (ABC) transporters are involved in a variety of translocation processes and some members have been implicated in drug resistance. Here we report the characterisation of the first T. cruzi ABCG transporter gene, named TcABCG1, which is over-expressed in parasite strains naturally resistant to BZ. Comparison of TcABCG1 gene sequence of two TcI BZ-resistant strains with CL Brener BZ-susceptible strain showed several single nucleotide polymorphisms, which determined 11 amino acid changes. CL Brener transfected with TcI transporter genes showed 40-47% increased resistance to BZ, whereas no statistical significant increment in drug resistance was observed when CL Brener was transfected with the homologous gene. Only in the parasites transfected with TcI genes there was 2-2.6-fold increased abundance of TcABCG1 transporter protein. The analysis in wild type strains also suggests that the level of TcABCG1 transporter is related to BZ natural resistance. The characteristics of untranslated regions of TcABCG1 genes of BZ-susceptible and resistant strains were investigated by computational tools.Chagas diseaseBenznidazole resistanceABCG transporterT. cruzi strainsTranscript abundanceSingle nucleotide polymorphismsDoença de ChagasTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12234/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALadeilton_brandão_etal_IOC_2015.pdfadeilton_brandão_etal_IOC_2015.pdfapplication/pdf1406867https://www.arca.fiocruz.br/bitstream/icict/12234/2/adeilton_brand%c3%a3o_etal_IOC_2015.pdfaf27900f8f05d46d7ea10200cbff5311MD52TEXTadeilton_brandão_etal_IOC_2015.pdf.txtadeilton_brandão_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
title	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
spellingShingle	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole Zingales, Bianca Chagas disease Benznidazole resistance ABCG transporter T. cruzi strains Transcript abundance Single nucleotide polymorphisms Doença de Chagas Trypanosoma cruzi
title_short	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
title_full	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
title_fullStr	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
title_full_unstemmed	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
title_sort	A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole
author	Zingales, Bianca
author_facet	Zingales, Bianca Araujo, Rafael Gomes Aquino Franco, Jaques Aguiar, Pedro Henrique Nascimento Nunes, Solange Lessa Silva, Marcelo Nunes Ienne, Susan Machado, Carlos Renato Brandão, Adeilton Moreno, Margoth
author_role	author
author2	Araujo, Rafael Gomes Aquino Franco, Jaques Aguiar, Pedro Henrique Nascimento Nunes, Solange Lessa Silva, Marcelo Nunes Ienne, Susan Machado, Carlos Renato Brandão, Adeilton Moreno, Margoth
author2_role	author author author author author author author author author
dc.contributor.author.fl_str_mv	Zingales, Bianca Araujo, Rafael Gomes Aquino Franco, Jaques Aguiar, Pedro Henrique Nascimento Nunes, Solange Lessa Silva, Marcelo Nunes Ienne, Susan Machado, Carlos Renato Brandão, Adeilton Moreno, Margoth
dc.subject.en.pt_BR.fl_str_mv	Chagas disease Benznidazole resistance ABCG transporter T. cruzi strains Transcript abundance Single nucleotide polymorphisms
topic	Chagas disease Benznidazole resistance ABCG transporter T. cruzi strains Transcript abundance Single nucleotide polymorphisms Doença de Chagas Trypanosoma cruzi
dc.subject.decs.pt_BR.fl_str_mv	Doença de Chagas Trypanosoma cruzi
description	Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.
publishDate	2015
dc.date.accessioned.fl_str_mv	2015-11-16T12:12:42Z
dc.date.available.fl_str_mv	2015-11-16T12:12:42Z
dc.date.issued.fl_str_mv	2015
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	ZINGALES, Bianca; et al. A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole. Mem Inst Oswaldo Cruz, v.110, n.3, p. 433-444, May 2015.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/12234
dc.identifier.issn.none.fl_str_mv	0074-0276
dc.identifier.doi.none.fl_str_mv	10.1590/0074-02760140407
dc.identifier.eissn.none.fl_str_mv	1678-8060
identifier_str_mv	ZINGALES, Bianca; et al. A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole. Mem Inst Oswaldo Cruz, v.110, n.3, p. 433-444, May 2015. 0074-0276 10.1590/0074-02760140407 1678-8060
url	https://www.arca.fiocruz.br/handle/icict/12234
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Fundação Oswaldo Cruz
publisher.none.fl_str_mv	Fundação Oswaldo Cruz
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/12234/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/12234/2/adeilton_brand%c3%a3o_etal_IOC_2015.pdf https://www.arca.fiocruz.br/bitstream/icict/12234/3/adeilton_brand%c3%a3o_etal_IOC_2015.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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A novel ABCG-like transporter of Trypanosoma cruzi is involved in natural resistance to benznidazole

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