Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/49058 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. |
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Silva, João Victor SilvaMoragas-Tellis, Carla J.Chagas, Maria S. S.Souza, Paulo Victor R.Moreira, Davyson L.Souza, Celeste S. F. deTeixeira, Kerolain F.Cenci, Arthur R.Oliveira, Aldo S. deSouza, Fernando AlmeidaBehrens, Maria D.Calabrese, Kátia S.2021-09-12T18:43:12Z2021-09-12T18:43:12Z2021SILVA, João Victor Silva et al. Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity. Biomedicine & Pharmacotherapy, v. 141, 111910, 12 p, July 2021.0753-3322https://www.arca.fiocruz.br/handle/icict/4905810.1016/j.biopha.2021.111910engElsevierLeishmania amazonensisMacrófagosÓxido nítricoÓxido nítrico sintaseLeishmania amazonensisMacrophagesNitric oxideNitric oxide synthaseCarajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Tecnológico de Farmacologia. Laboratório de Produtos Naturais para Saúde Pública,. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Tecnológico de Farmacologia. Laboratório de Produtos Naturais para Saúde Pública,. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Tecnológico de Farmacologia. Laboratório de Produtos Naturais para Saúde Pública,. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Farmanguinhos. Aluno do Programa de Pós-Graduação em Pesquisa Translacional em Drogas e Medicamentos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Tecnológico de Farmacologia. Laboratório de Produtos Naturais para Saúde Pública,. Rio de Janeiro, RJ, Brasil / Instituto de Pesquisa do jardim Botânico. Jardim Botânico. Diretoria de Pesquisa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Santa Catarina. Departamento de Educação e Ciências Exatas. Blumenau, SC, BrasilUniversidade Federal de Santa Catarina. Departamento de Educação e Ciências Exatas. Blumenau, SC, BrasilUniversidade Federal de Santa Catarina. Departamento de Educação e Ciências Exatas. Blumenau, SC, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. Programa de Pós Graduação em Zootecnia. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Tecnológico de Farmacologia. Laboratório de Produtos Naturais para Saúde Pública,. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Leishmaniasis is a group of neglected tropical diseases whose treatment with antimonials bears limitations and has changed little in over 80 years. Medicinal plants have been evaluated as a therapeutic alternative for leishmaniasis. Arrabidaea chica is popularly used as a wound healing and antiparasitic agent, especially as leishmanicidal agent. This study examined the leishmanicidal activity of a crude extract (ACCE), an anthocyanidin-rich fraction (ACAF), and three isolated anthocyanidins from A. chica: carajurin, 3′ -hydroxy carajurone, and carajurone. We evaluated the antileishmanial activity against promastigote and intracellular amastigote forms of Leishmania amazonensis and determined cytotoxicity in BALB/c peritoneal macrophages, as well as nitrite quantification, using the Griess method. Molecular docking was carried out to evaluate in teractions of carajurin at the nitric oxide synthase enzyme. All compounds were active against promastigotes after 72 h, with IC50 values of 101.5 ± 0.06 μg/mL for ACCE and 4.976 ± 1.09 μg/mL for ACAF. Anthocyanidins carajurin, 3′ -hydroxy-carajurone, and carajurone had IC50 values of 3.66 ± 1.16, 22.70 ± 1.20, and 28.28 ± 0.07 μg/mL, respectively. The cytotoxicity assay after 72 h showed results ranging from 9.640 to 66.74 µg/mL for anthocyanidins. ACAF and carajurin showed selectivity against intracellular amastigote forms (SI> 10), with low cytotoxicity within 24 h, a statistically significant reduction in all infection parameters, and induced nitrite production. Molecular docking studies were developed to understand a possible mechanism of activation of the nitric oxide synthase enzyme, which leads to an increase in the production of nitric oxide observed in the other experiments reported. These results encourage us to suggest carajurin as a biological marker of A. chica.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/49058/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJoaoVitorSilva_FernandoSouza_etal_IOC_2021.pdfJoaoVitorSilva_FernandoSouza_etal_IOC_2021.pdfapplication/pdf5027852https://www.arca.fiocruz.br/bitstream/icict/49058/2/JoaoVitorSilva_FernandoSouza_etal_IOC_2021.pdf120f47e15bd1d489270344ad068f54a1MD52TEXTJoaoVitorSilva_FernandoSouza_etal_IOC_2021.pdf.txtJoaoVitorSilva_FernandoSouza_etal_IOC_2021.pdf.txtExtracted texttext/plain69331https://www.arca.fiocruz.br/bitstream/icict/49058/3/JoaoVitorSilva_FernandoSouza_etal_IOC_2021.pdf.txtdac7e801fc2bd1d56d96df1e06d8f3caMD53icict/490582021-09-13 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| dc.title.pt_BR.fl_str_mv |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| title |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| spellingShingle |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity Silva, João Victor Silva Leishmania amazonensis Macrófagos Óxido nítrico Óxido nítrico sintase Leishmania amazonensis Macrophages Nitric oxide Nitric oxide synthase |
| title_short |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| title_full |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| title_fullStr |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| title_full_unstemmed |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| title_sort |
Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity |
| author |
Silva, João Victor Silva |
| author_facet |
Silva, João Victor Silva Moragas-Tellis, Carla J. Chagas, Maria S. S. Souza, Paulo Victor R. Moreira, Davyson L. Souza, Celeste S. F. de Teixeira, Kerolain F. Cenci, Arthur R. Oliveira, Aldo S. de Souza, Fernando Almeida Behrens, Maria D. Calabrese, Kátia S. |
| author_role |
author |
| author2 |
Moragas-Tellis, Carla J. Chagas, Maria S. S. Souza, Paulo Victor R. Moreira, Davyson L. Souza, Celeste S. F. de Teixeira, Kerolain F. Cenci, Arthur R. Oliveira, Aldo S. de Souza, Fernando Almeida Behrens, Maria D. Calabrese, Kátia S. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, João Victor Silva Moragas-Tellis, Carla J. Chagas, Maria S. S. Souza, Paulo Victor R. Moreira, Davyson L. Souza, Celeste S. F. de Teixeira, Kerolain F. Cenci, Arthur R. Oliveira, Aldo S. de Souza, Fernando Almeida Behrens, Maria D. Calabrese, Kátia S. |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania amazonensis Macrófagos Óxido nítrico Óxido nítrico sintase |
| topic |
Leishmania amazonensis Macrófagos Óxido nítrico Óxido nítrico sintase Leishmania amazonensis Macrophages Nitric oxide Nitric oxide synthase |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania amazonensis Macrophages Nitric oxide Nitric oxide synthase |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-09-12T18:43:12Z |
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2021-09-12T18:43:12Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA, João Victor Silva et al. Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity. Biomedicine & Pharmacotherapy, v. 141, 111910, 12 p, July 2021. |
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https://www.arca.fiocruz.br/handle/icict/49058 |
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0753-3322 |
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10.1016/j.biopha.2021.111910 |
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SILVA, João Victor Silva et al. Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity. Biomedicine & Pharmacotherapy, v. 141, 111910, 12 p, July 2021. 0753-3322 10.1016/j.biopha.2021.111910 |
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