Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection

Detalhes bibliográficos
Autor(a) principal: Gomes, Yago
Data de Publicação: 2020
Outros Autores: Caterino-de-Araujo, Adele, Campos, Karoline, Gonçalves, Maria Gisele, Leite, Ana Claudia, Lima, Marco Antonio, Araújo, Abelardo, Silva, Marcus Tulius, Espíndola, Otávio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44292
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.
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spelling Gomes, YagoCaterino-de-Araujo, AdeleCampos, KarolineGonçalves, Maria GiseleLeite, Ana ClaudiaLima, Marco AntonioAraújo, AbelardoSilva, Marcus TuliusEspíndola, Otávio2020-10-31T18:07:41Z2020-10-31T18:07:41Z2020GOMES, Yago et al. Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection. Viruses, v. 12, n. 9, p. 1-15, 2020.1999-4915https://www.arca.fiocruz.br/handle/icict/4429210.3390/v12090981engMDPILoop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Adolfo Lutz Institute. Immunology Center. Laboratory of HTLV Research. São Paulo, SP, Brasil.Adolfo Lutz Institute. Immunology Center. Laboratory of HTLV Research. São Paulo, SP, Brasil.Adolfo Lutz Institute. Immunology Center. Laboratory of HTLV Research. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Laboratory diagnosis of human T-lymphotropic viruses (HTLV) 1 and 2 infection is performed by serological screening and further confirmation with serological or molecular assays. Thus, we developed a loop-mediated isothermal nucleic acid amplification (LAMP) assay for the detection of HTLV-1/2 in blood samples. The sensitivity and accuracy of HTLV-1/2 LAMP were defined with DNA samples from individuals infected with HTLV-1 (n = 125), HTLV-2 (n = 19), and coinfected with HIV (n = 82), and compared with real-time polymerase chain reaction (qPCR) and PCR-restriction fragment length polymorphism (RFLP). The overall accuracy of HTLV-1/2 LAMP (95% CI 74.8-85.5%) was slightly superior to qPCR (95% CI 69.5-81.1%) and similar to PCR-RFLP (95% CI 79.5-89.3%). The sensitivity of LAMP was greater for HTLV-1 (95% CI 83.2-93.4%) than for HTLV-2 (95% CI 43.2-70.8%). This was also observed in qPCR and PCR-RFLP, which was associated with the commonly lower HTLV-2 proviral load. All molecular assays tested showed better results with samples from HTLV-1/2 mono-infected individuals compared with HIV-coinfected patients, who present lower CD4 T-cell counts. In conclusion, HTLV-1/2 LAMP had similar to superior performance than PCR-based assays, and therefore may represent an attractive alternative for HTLV-1/2 diagnosis due to reduced working time and costs, and the simple infrastructure needed.HTLV-1HTLV-2LAMPConfirmatory diagnosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/44292/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALve_Yago_Gomes_etal_INI_2020.pdfve_Yago_Gomes_etal_INI_2020.pdfapplication/pdf2419423https://www.arca.fiocruz.br/bitstream/icict/44292/2/ve_Yago_Gomes_etal_INI_2020.pdfaa656c901bab4e1ecb1482aee4fe7250MD52TEXTve_Yago_Gomes_etal_INI_2020.pdf.txtve_Yago_Gomes_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
title Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
spellingShingle Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
Gomes, Yago
HTLV-1
HTLV-2
LAMP
Confirmatory diagnosis
title_short Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
title_full Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
title_fullStr Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
title_full_unstemmed Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
title_sort Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection
author Gomes, Yago
author_facet Gomes, Yago
Caterino-de-Araujo, Adele
Campos, Karoline
Gonçalves, Maria Gisele
Leite, Ana Claudia
Lima, Marco Antonio
Araújo, Abelardo
Silva, Marcus Tulius
Espíndola, Otávio
author_role author
author2 Caterino-de-Araujo, Adele
Campos, Karoline
Gonçalves, Maria Gisele
Leite, Ana Claudia
Lima, Marco Antonio
Araújo, Abelardo
Silva, Marcus Tulius
Espíndola, Otávio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Yago
Caterino-de-Araujo, Adele
Campos, Karoline
Gonçalves, Maria Gisele
Leite, Ana Claudia
Lima, Marco Antonio
Araújo, Abelardo
Silva, Marcus Tulius
Espíndola, Otávio
dc.subject.en.pt_BR.fl_str_mv HTLV-1
HTLV-2
LAMP
Confirmatory diagnosis
topic HTLV-1
HTLV-2
LAMP
Confirmatory diagnosis
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-31T18:07:41Z
dc.date.available.fl_str_mv 2020-10-31T18:07:41Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, Yago et al. Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection. Viruses, v. 12, n. 9, p. 1-15, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44292
dc.identifier.issn.pt_BR.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v12090981
identifier_str_mv GOMES, Yago et al. Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection. Viruses, v. 12, n. 9, p. 1-15, 2020.
1999-4915
10.3390/v12090981
url https://www.arca.fiocruz.br/handle/icict/44292
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/44292/2/ve_Yago_Gomes_etal_INI_2020.pdf
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