Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes

Detalhes bibliográficos
Autor(a) principal: Fiorotti, Jéssica
Data de Publicação: 2019
Outros Autores: Menna-Barreto, Rubem F. S., Gôlo, Patrícia Silva, Rodrigues, Caio Junior Balduino Coutinho, Bitencourt, Ricardo Oliveira Barbosa, Morena, Diva Denelle Spadacci, Angelo, Isabele da Costa, Bittencourt, Vânia Rita Elias Pinheiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35643
Resumo: Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.
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spelling Fiorotti, JéssicaMenna-Barreto, Rubem F. S.Gôlo, Patrícia SilvaRodrigues, Caio Junior Balduino CoutinhoBitencourt, Ricardo Oliveira BarbosaMorena, Diva Denelle SpadacciAngelo, Isabele da CostaBittencourt, Vânia Rita Elias Pinheiro2019-09-17T16:20:55Z2019-09-17T16:20:55Z2019FIOROTTI, Jéssica et al. Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes. Frontiers in Physiology, v. 10, p. 1-17, May 2019.1664-042Xhttps://www.arca.fiocruz.br/handle/icict/3564310.3389/fphys.2019.00654engFrontiers MediaFungos entomopatogênicosInfecção por fungosMorte celularImunidadeCarrapatosEntomopathogenic fungiCell deathFungal infectionTickImmunityUltrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Parasitologia Animal. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.Instituto Butantan. Laboratório de Fisiopatologia. São Paulo, SP, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Epidemiologia e Saúde Pública. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Parasitologia Animal. Seropédica, RJ, Brasil.Metarhizium is an entomopathogenic fungus widely employed in the biological control of arthropods. Hemocytes present in the hemolymph of invertebrates are the cells involved in the immune response of arthropods. Despite this, knowledge about Rhipicephalus microplus hemocytes morphological aspects as well as their role in response to the fungal infection is scarce. The present study aimed to analyze the hemocytes of R. microplus females after Metarhizium robertsii infection, using light and electron microscopy approaches associated with the cytotoxicity evaluation. Five types of hemocytes (prohemocytes, spherulocytes, plasmatocytes, granulocytes, and oenocytoids) were described in the hemolymph of uninfected ticks, while only prohemocytes, granulocytes, and plasmatocytes were observed in fungus-infected tick females. Twenty-four hours after the fungal infection, only granulocytes and plasmatocytes were detected in the transmission electron microscopy analysis. Hemocytes from fungus-infected tick females showed several cytoplasmic vacuoles with different electron densities, and lipid droplets in close contact to low electron density vacuoles, as well as the formation of autophagosomes and subcellular material in different stages of degradation could also be observed. M. robertsii propagules were more toxic to tick hemocytes in the highest concentration tested (1.0 × 108 conidia mL-1). Interestingly, the lowest fungus concentration did not affect significantly the cell viability. Microanalysis showed that cells granules from fungus-infected and uninfected ticks had similar composition. This study addressed the first report of fungal cytotoxicity analyzing ultrastructural effects on hemocytes of R. microplus infected with entomopathogenic fungi. These results open new perspectives for the comprehension of ticks physiology and pathology, allowing the identification of new targets for the biological control.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35643/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRubemFS_MennaBarreto_etal_IOC_2019.pdfRubemFS_MennaBarreto_etal_IOC_2019.pdfapplication/pdf10011051https://www.arca.fiocruz.br/bitstream/icict/35643/2/RubemFS_MennaBarreto_etal_IOC_2019.pdfc614c7b8232bbc7c1416f7028a7a45f1MD52TEXTRubemFS_MennaBarreto_etal_IOC_2019.pdf.txtRubemFS_MennaBarreto_etal_IOC_2019.pdf.txtExtracted texttext/plain83925https://www.arca.fiocruz.br/bitstream/icict/35643/3/RubemFS_MennaBarreto_etal_IOC_2019.pdf.txt5074dab67ed1de860ad7b5ab42310f43MD53icict/356432019-09-18 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dc.title.pt_BR.fl_str_mv Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
title Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
spellingShingle Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
Fiorotti, Jéssica
Fungos entomopatogênicos
Infecção por fungos
Morte celular
Imunidade
Carrapatos
Entomopathogenic fungi
Cell death
Fungal infection
Tick
Immunity
title_short Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
title_full Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
title_fullStr Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
title_full_unstemmed Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
title_sort Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes
author Fiorotti, Jéssica
author_facet Fiorotti, Jéssica
Menna-Barreto, Rubem F. S.
Gôlo, Patrícia Silva
Rodrigues, Caio Junior Balduino Coutinho
Bitencourt, Ricardo Oliveira Barbosa
Morena, Diva Denelle Spadacci
Angelo, Isabele da Costa
Bittencourt, Vânia Rita Elias Pinheiro
author_role author
author2 Menna-Barreto, Rubem F. S.
Gôlo, Patrícia Silva
Rodrigues, Caio Junior Balduino Coutinho
Bitencourt, Ricardo Oliveira Barbosa
Morena, Diva Denelle Spadacci
Angelo, Isabele da Costa
Bittencourt, Vânia Rita Elias Pinheiro
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fiorotti, Jéssica
Menna-Barreto, Rubem F. S.
Gôlo, Patrícia Silva
Rodrigues, Caio Junior Balduino Coutinho
Bitencourt, Ricardo Oliveira Barbosa
Morena, Diva Denelle Spadacci
Angelo, Isabele da Costa
Bittencourt, Vânia Rita Elias Pinheiro
dc.subject.other.pt_BR.fl_str_mv Fungos entomopatogênicos
Infecção por fungos
Morte celular
Imunidade
Carrapatos
topic Fungos entomopatogênicos
Infecção por fungos
Morte celular
Imunidade
Carrapatos
Entomopathogenic fungi
Cell death
Fungal infection
Tick
Immunity
dc.subject.en.pt_BR.fl_str_mv Entomopathogenic fungi
Cell death
Fungal infection
Tick
Immunity
description Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-17T16:20:55Z
dc.date.available.fl_str_mv 2019-09-17T16:20:55Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIOROTTI, Jéssica et al. Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes. Frontiers in Physiology, v. 10, p. 1-17, May 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35643
dc.identifier.issn.pt_BR.fl_str_mv 1664-042X
dc.identifier.doi.none.fl_str_mv 10.3389/fphys.2019.00654
identifier_str_mv FIOROTTI, Jéssica et al. Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes. Frontiers in Physiology, v. 10, p. 1-17, May 2019.
1664-042X
10.3389/fphys.2019.00654
url https://www.arca.fiocruz.br/handle/icict/35643
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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