Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46878 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. |
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Bandeira, Derick MendesCaldas, Gabriela CardosoJácome, Fernanda CunhaRasinhas, Arthur da CostaAlmeida, Ana Luisa Teixeira deTourinho, Renata SantosAmorim, Juliana Fernandes da SilvaTrindade, Gisela FreitasBarth, Ortrud MonikaVieira, Debora Ferreira Barreto2021-04-25T18:24:26Z2021-04-25T18:24:26Z2021BANDEIRA, Derick Mendes et al. Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice, Resarch Square, p. 1-11, 2021.https://www.arca.fiocruz.br/handle/icict/4687810.34119/bjhrv4n1-160engResarch SquareZika virus RNADetecção do RNAAspectos clínicosTecidos de BALB/c miceVirologiaqRT-PCRDetection of Zika virus RNAClinical aspectsBALB/c miceTissuesqRT-PCRVirologyClinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.The aim of the present study was to infect BALB/c mice with ZIKV to evaluate clinical signs and to quantify the viral load in different tissues at three different kinetic points. For this purpose, fifteen mice were inoculated with a 100µl of a viral solution and five other mice were used as negative controls. After euthanasia, nine different tissues were collected and macerated for RNA extraction and quantification. The infections were not lethal. Some of them, however, showed great agitation, hair bristling and itchy skin. Viral RNA was detected in one heart sample, eight samples of spleen and two of skeletal muscle. Seven out of eleven positive samples were from mice euthanized on the third day after infection. Only spleen yielded positive results at a later time of infection. These results contribute to a better understanding of clinical signs and replication dynamics of Zika virus in different organs of BALB/c mice, which are still scarce data in the scientific literature.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46878/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALOrtrudMonika_DeboraFB_Vieira_etal_IOC_2021.pdfOrtrudMonika_DeboraFB_Vieira_etal_IOC_2021.pdfapplication/pdf85757https://www.arca.fiocruz.br/bitstream/icict/46878/2/OrtrudMonika_DeboraFB_Vieira_etal_IOC_2021.pdfc356a8a526aaf47cb938fa57e5620f23MD52TEXTOrtrudMonika_DeboraFB_Vieira_etal_IOC_2021.pdf.txtOrtrudMonika_DeboraFB_Vieira_etal_IOC_2021.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| title |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| spellingShingle |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice Bandeira, Derick Mendes Zika virus RNA Detecção do RNA Aspectos clínicos Tecidos de BALB/c mice Virologia qRT-PCR Detection of Zika virus RNA Clinical aspects BALB/c mice Tissues qRT-PCR Virology |
| title_short |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| title_full |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| title_fullStr |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| title_full_unstemmed |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| title_sort |
Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice |
| author |
Bandeira, Derick Mendes |
| author_facet |
Bandeira, Derick Mendes Caldas, Gabriela Cardoso Jácome, Fernanda Cunha Rasinhas, Arthur da Costa Almeida, Ana Luisa Teixeira de Tourinho, Renata Santos Amorim, Juliana Fernandes da Silva Trindade, Gisela Freitas Barth, Ortrud Monika Vieira, Debora Ferreira Barreto |
| author_role |
author |
| author2 |
Caldas, Gabriela Cardoso Jácome, Fernanda Cunha Rasinhas, Arthur da Costa Almeida, Ana Luisa Teixeira de Tourinho, Renata Santos Amorim, Juliana Fernandes da Silva Trindade, Gisela Freitas Barth, Ortrud Monika Vieira, Debora Ferreira Barreto |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bandeira, Derick Mendes Caldas, Gabriela Cardoso Jácome, Fernanda Cunha Rasinhas, Arthur da Costa Almeida, Ana Luisa Teixeira de Tourinho, Renata Santos Amorim, Juliana Fernandes da Silva Trindade, Gisela Freitas Barth, Ortrud Monika Vieira, Debora Ferreira Barreto |
| dc.subject.other.pt_BR.fl_str_mv |
Zika virus RNA Detecção do RNA Aspectos clínicos Tecidos de BALB/c mice Virologia qRT-PCR |
| topic |
Zika virus RNA Detecção do RNA Aspectos clínicos Tecidos de BALB/c mice Virologia qRT-PCR Detection of Zika virus RNA Clinical aspects BALB/c mice Tissues qRT-PCR Virology |
| dc.subject.en.pt_BR.fl_str_mv |
Detection of Zika virus RNA Clinical aspects BALB/c mice Tissues qRT-PCR Virology |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-04-25T18:24:26Z |
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2021-04-25T18:24:26Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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BANDEIRA, Derick Mendes et al. Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice, Resarch Square, p. 1-11, 2021. |
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https://www.arca.fiocruz.br/handle/icict/46878 |
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10.34119/bjhrv4n1-160 |
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BANDEIRA, Derick Mendes et al. Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice, Resarch Square, p. 1-11, 2021. 10.34119/bjhrv4n1-160 |
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Resarch Square |
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Resarch Square |
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