Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?

Detalhes bibliográficos
Autor(a) principal: Morgado, Fernanda Nazaré
Data de Publicação: 2015
Outros Autores: Nascimento, Michelle T. C., Saraiva, Elvira M., Oliveira-Ribeiro, Carla de, Madeira, Maria de Fátima, Costa-Santos, Marcela da, Vasconcellos, Erica C. F., Pimentel, Maria Ines F., Lyra, Marcelo Rosandiski, Schubach, Armando de Oliveira, Conceição-Silva, Fátima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12924
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Morgado, Fernanda NazaréNascimento, Michelle T. C.Saraiva, Elvira M.Oliveira-Ribeiro, Carla deMadeira, Maria de FátimaCosta-Santos, Marcela daVasconcellos, Erica C. F.Pimentel, Maria Ines F.Lyra, Marcelo RosandiskiSchubach, Armando de OliveiraConceição-Silva, Fátima2016-03-01T16:19:02Z2016-03-01T16:19:02Z2015MORGADO, Fernanda Nazaré et al. Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions? Plos One, v. 10, n. 7, p. 1-15, July 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1292410.1371/journal. pone.0133063engPublic Library of ScienceAre Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas - INI. Laboratório de Vigilância em Leishmanioses - VigiLeish. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas - INI. Laboratório de Vigilância em Leishmanioses - VigiLeish. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas - INI. Laboratório de Vigilância em Leishmanioses - VigiLeish. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas - INI. Laboratório de Vigilância em Leishmanioses - VigiLeish. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas - INI. Laboratório de Vigilância em Leishmanioses - VigiLeish. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas - INI. Laboratório de Vigilância em Leishmanioses - VigiLeish. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Neutrophil extracellular traps (NETs) have been described as a network of extracellular fibers composed by DNA, histones and various proteins/enzymes. Studies have demonstrated that NETs could be responsible for the trapping and elimination of a variety of infectious agents. In order to verify the presence of NETs in American tegumentary leishmaniasis (ATL) and their relationship with the presence of amastigotes we evaluated active cutaneous lesions of 35 patients before treatment by the detection of parasites, neutrophils (neutrophil elastase) and histones through immunohistochemistry and confocal immunofluorescence. Intact neutrophils could be detected in all ATL lesions. NETs were present in 27 patients (median 1.1; range from 0.1 to 23.5/mm2 ) with lesion duration ranging from one to seven months. NETs were in close proximity with neutrophils (r = 0.586; p = 0.0001) and amastigotes (r = 0.710; p = 0.0001). Two patterns of NET formation were detected: small homogeneously distributed networks observed in all lesions; and large structures that could be visualized at a lower magnification in lesions presenting at least 20% of neutrophils. Lesions presenting the larger NET formation showed high parasite detection. A correlation between NET size and the number of intact amastigotes was observed (p=0.02). As we detected an association between NET and amastigotes, our results suggest that neutrophil migration and NET formation could be stimulated and maintained by stimuli derived from the parasite burden/parasite antigen in the extracellular environment. The observation of areas containing only antigens not intermingled with NETs (elastase and histone) suggests that the involvement of these structures in the control of parasite burden is a dynamic process in which the formation of NETs is exhausted with the destruction of the parasites. Since NETs were also associated with granulomas, this trapping would favor the activity of macrophages in order to control the parasite burden.Neutrophil Extracellular TrapsAmerican Tegumentary LeishmaniasisParasite ControlLeishmaniasisArmadilhas ExtracelularesLeishmanioseParasitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12924/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfernanda_morgado_etal_IOC_2015.PDFfernanda_morgado_etal_IOC_2015.PDFapplication/pdf4398456https://www.arca.fiocruz.br/bitstream/icict/12924/2/fernanda_morgado_etal_IOC_2015.PDFa37e8f7910845e09d79ad8b0f51dc79eMD52TEXTfernanda_morgado_etal_IOC_2015.PDF.txtfernanda_morgado_etal_IOC_2015.PDF.txtExtracted 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dc.title.pt_BR.fl_str_mv Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
title Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
spellingShingle Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
Morgado, Fernanda Nazaré
Neutrophil Extracellular Traps
American Tegumentary Leishmaniasis
Parasite Control
Leishmaniasis
Armadilhas Extracelulares
Leishmaniose
Parasitos
title_short Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
title_full Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
title_fullStr Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
title_full_unstemmed Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
title_sort Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions?
author Morgado, Fernanda Nazaré
author_facet Morgado, Fernanda Nazaré
Nascimento, Michelle T. C.
Saraiva, Elvira M.
Oliveira-Ribeiro, Carla de
Madeira, Maria de Fátima
Costa-Santos, Marcela da
Vasconcellos, Erica C. F.
Pimentel, Maria Ines F.
Lyra, Marcelo Rosandiski
Schubach, Armando de Oliveira
Conceição-Silva, Fátima
author_role author
author2 Nascimento, Michelle T. C.
Saraiva, Elvira M.
Oliveira-Ribeiro, Carla de
Madeira, Maria de Fátima
Costa-Santos, Marcela da
Vasconcellos, Erica C. F.
Pimentel, Maria Ines F.
Lyra, Marcelo Rosandiski
Schubach, Armando de Oliveira
Conceição-Silva, Fátima
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morgado, Fernanda Nazaré
Nascimento, Michelle T. C.
Saraiva, Elvira M.
Oliveira-Ribeiro, Carla de
Madeira, Maria de Fátima
Costa-Santos, Marcela da
Vasconcellos, Erica C. F.
Pimentel, Maria Ines F.
Lyra, Marcelo Rosandiski
Schubach, Armando de Oliveira
Conceição-Silva, Fátima
dc.subject.en.pt_BR.fl_str_mv Neutrophil Extracellular Traps
American Tegumentary Leishmaniasis
Parasite Control
Leishmaniasis
topic Neutrophil Extracellular Traps
American Tegumentary Leishmaniasis
Parasite Control
Leishmaniasis
Armadilhas Extracelulares
Leishmaniose
Parasitos
dc.subject.decs.pt_BR.fl_str_mv Armadilhas Extracelulares
Leishmaniose
Parasitos
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-01T16:19:02Z
dc.date.available.fl_str_mv 2016-03-01T16:19:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORGADO, Fernanda Nazaré et al. Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions? Plos One, v. 10, n. 7, p. 1-15, July 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12924
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal. pone.0133063
identifier_str_mv MORGADO, Fernanda Nazaré et al. Are Neutrophil Extracellular Traps Playing a Role in the Parasite Control in Active American Tegumentary Leishmaniasis Lesions? Plos One, v. 10, n. 7, p. 1-15, July 2015.
1932-6203
10.1371/journal. pone.0133063
url https://www.arca.fiocruz.br/handle/icict/12924
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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