Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children

Detalhes bibliográficos
Autor(a) principal: Brustoloni, Yvone Maia
Data de Publicação: 2007
Outros Autores: Lima, Rosimar Batista, Cunha, Rivaldo Venâncio da, Dorval, Maria Elizabeth, Oshiro, Elisa Teruya, Oliveira, Ana Lúcia Lyrio de, Pirmez, Claude
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28986
Resumo: Universidade Federal de Mato Grosso do Sul. Departamento de Pediatria. Campo Grande, MS, Brasil.
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spelling Brustoloni, Yvone MaiaLima, Rosimar BatistaCunha, Rivaldo Venâncio daDorval, Maria ElizabethOshiro, Elisa TeruyaOliveira, Ana Lúcia Lyrio dePirmez, Claude2018-09-22T16:37:01Z2018-09-22T16:37:01Z2007BRUSTOLONI, Yvonne Maia; et al. Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, v.102, n.4, p.497-500, June 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/289861678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Leishmaniose visceralReação em Cadeia da PolimeraseDiagnósticovisceral leishmaniasispolymerase chain reactiondiagnosisSensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in childreninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Mato Grosso do Sul. Departamento de Pediatria. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Patologia. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Patologia. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28986/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrosimar_lima_etal-IOC_2007.pdfrosimar_lima_etal-IOC_2007.pdfapplication/pdf268541https://www.arca.fiocruz.br/bitstream/icict/28986/2/rosimar_lima_etal-IOC_2007.pdfa1bb41b9b9cdde9de4ae873f90249dbaMD52TEXTrosimar_lima_etal-IOC_2007.pdf.txtrosimar_lima_etal-IOC_2007.pdf.txtExtracted texttext/plain17425https://www.arca.fiocruz.br/bitstream/icict/28986/3/rosimar_lima_etal-IOC_2007.pdf.txt8ebf4a36e1bd61fa17260ce274ff0923MD53icict/289862018-09-23 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dc.title.pt_BR.fl_str_mv Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
title Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
spellingShingle Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
Brustoloni, Yvone Maia
Leishmaniose visceral
Reação em Cadeia da Polimerase
Diagnóstico
visceral leishmaniasis
polymerase chain reaction
diagnosis
title_short Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
title_full Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
title_fullStr Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
title_full_unstemmed Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
title_sort Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
author Brustoloni, Yvone Maia
author_facet Brustoloni, Yvone Maia
Lima, Rosimar Batista
Cunha, Rivaldo Venâncio da
Dorval, Maria Elizabeth
Oshiro, Elisa Teruya
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
author_role author
author2 Lima, Rosimar Batista
Cunha, Rivaldo Venâncio da
Dorval, Maria Elizabeth
Oshiro, Elisa Teruya
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brustoloni, Yvone Maia
Lima, Rosimar Batista
Cunha, Rivaldo Venâncio da
Dorval, Maria Elizabeth
Oshiro, Elisa Teruya
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral
Reação em Cadeia da Polimerase
Diagnóstico
topic Leishmaniose visceral
Reação em Cadeia da Polimerase
Diagnóstico
visceral leishmaniasis
polymerase chain reaction
diagnosis
dc.subject.en.pt_BR.fl_str_mv visceral leishmaniasis
polymerase chain reaction
diagnosis
description Universidade Federal de Mato Grosso do Sul. Departamento de Pediatria. Campo Grande, MS, Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2018-09-22T16:37:01Z
dc.date.available.fl_str_mv 2018-09-22T16:37:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRUSTOLONI, Yvonne Maia; et al. Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, v.102, n.4, p.497-500, June 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28986
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BRUSTOLONI, Yvonne Maia; et al. Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, v.102, n.4, p.497-500, June 2009.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/28986
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/28986/3/rosimar_lima_etal-IOC_2007.pdf.txt
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