A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation

Detalhes bibliográficos
Autor(a) principal: Cota, Betania Barros
Data de Publicação: 2008
Outros Autores: Rosa, Luiz Henrique, Fagundes, Elaine Maria Souza, Martins Filho, Olindo Assis, Oliveira, Rodrigo Correa de, Romanha, Alvaro José, Rosa, Carlos Augusto, Zani, Carlos Leomar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53627
Resumo: FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS
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spelling Cota, Betania BarrosRosa, Luiz HenriqueFagundes, Elaine Maria SouzaMartins Filho, Olindo AssisOliveira, Rodrigo Correa deRomanha, Alvaro JoséRosa, Carlos AugustoZani, Carlos Leomar2022-07-04T17:02:00Z2022-07-04T17:02:00Z2008COTA, Betania Barros et al. A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation. Mem. Inst. Oswaldo Cruz, v. 103, n. 3, p. 263-270, 2008 • https://doi.org/10.1590/S0074-027620080003000070074-0276https://www.arca.fiocruz.br/handle/icict/5362710.1590/S0074-02762008000300007FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAISFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas, Departamento de Microbiologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrasilThe fungus Lentinus strigosus (Pegler 1983) (Polyporaceae, basidiomycete) was selected in a screen for inhibitory activity on Trypanosoma cruzi trypanothione reductase (TR). The crude extract of L. strigosus was able to completely inhibit TR at 20 mu g/ml. Two triquinane sesquiterpenoids (dihydrohypnophilin and hypnophilin), in addition to two panepoxydol derivatives (neopanepoxydol and panepoxydone), were isolated using a bioassay-guided fractionation protocol. Hypnophilin and panepoxydone displayed IC(50) values of 0.8 and 38.9 mu M in the TR assay, respectively, while the other two compounds were inactive. The activity of hypnophilin was confirmed in a secondary assay with the intracellular amastigote forms of T. cruzi, in which it presented an IC(50) value of 2.5 mu M. Quantitative flow cytometry experiments demonstrated that hypnophilin at 4 mu M also reduced the proliferation of human peripheral blood monocluear cells (PBMC) stimulated with phytohemaglutinin, without any apparent interference on the viability of lymphocytes and monocytes. As the host immune response plays a pivotal role in the adverse events triggered by antigen release during treatment with trypanocidal drugs, the ability of hypnophilin to kill the intracellular forms of T. cruzi while modulating human PBMC proliferation suggests that this terpenoid may be a promising prototype for the development of new chemotherapeutical agents for Chagas diseaseengMinistério da SaúdeA potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlefungal natural productsChagas diseasedrug discoveryimmunomodulatorsBasidiomycotainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/53627/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALA potent trypanocidal component from the fungus Lentinus strigosus.pdfA potent trypanocidal component from the fungus Lentinus strigosus.pdfapplication/pdf648388https://www.arca.fiocruz.br/bitstream/icict/53627/2/A%20potent%20trypanocidal%20component%20from%20the%20fungus%20Lentinus%20strigosus.pdf1073e4f5567f0d3a90e7a6a932e1f6b4MD52icict/536272022-07-04 14:02:00.768oai:www.arca.fiocruz.br:icict/53627Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-07-04T17:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
title A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
spellingShingle A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
Cota, Betania Barros
fungal natural products
Chagas disease
drug discovery
immunomodulators
Basidiomycota
title_short A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
title_full A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
title_fullStr A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
title_full_unstemmed A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
title_sort A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
author Cota, Betania Barros
author_facet Cota, Betania Barros
Rosa, Luiz Henrique
Fagundes, Elaine Maria Souza
Martins Filho, Olindo Assis
Oliveira, Rodrigo Correa de
Romanha, Alvaro José
Rosa, Carlos Augusto
Zani, Carlos Leomar
author_role author
author2 Rosa, Luiz Henrique
Fagundes, Elaine Maria Souza
Martins Filho, Olindo Assis
Oliveira, Rodrigo Correa de
Romanha, Alvaro José
Rosa, Carlos Augusto
Zani, Carlos Leomar
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cota, Betania Barros
Rosa, Luiz Henrique
Fagundes, Elaine Maria Souza
Martins Filho, Olindo Assis
Oliveira, Rodrigo Correa de
Romanha, Alvaro José
Rosa, Carlos Augusto
Zani, Carlos Leomar
dc.subject.en.pt_BR.fl_str_mv fungal natural products
Chagas disease
drug discovery
immunomodulators
Basidiomycota
topic fungal natural products
Chagas disease
drug discovery
immunomodulators
Basidiomycota
description FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2022-07-04T17:02:00Z
dc.date.available.fl_str_mv 2022-07-04T17:02:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COTA, Betania Barros et al. A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation. Mem. Inst. Oswaldo Cruz, v. 103, n. 3, p. 263-270, 2008 • https://doi.org/10.1590/S0074-02762008000300007
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53627
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762008000300007
identifier_str_mv COTA, Betania Barros et al. A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation. Mem. Inst. Oswaldo Cruz, v. 103, n. 3, p. 263-270, 2008 • https://doi.org/10.1590/S0074-02762008000300007
0074-0276
10.1590/S0074-02762008000300007
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dc.publisher.none.fl_str_mv Ministério da Saúde
publisher.none.fl_str_mv Ministério da Saúde
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