Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20067 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Referência para Doença de Chagas. Recife, PE, Brasil. |
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Santos, Fred Luciano NevesSouza, Wayner Vieira deBarros, Michelle da SilvaNakazawa, MineoKrieger, Marco AurélioGomes, Yara de Miranda2017-07-13T12:29:32Z2017-07-13T12:29:32Z2016SANTOS, F. L. N. et al. Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests. The American Journal of Tropical Medicine and Hygiene, v. 94, n. 5, p. 1034–1039, 4 maio 2016. DOI 10.4269/ajtmh.15-0820.1476-1645https://www.arca.fiocruz.br/handle/icict/2006710.4269/ajtmh.15-0820porCardiomiopatia ChagásicaTécnicas ImunoenzimáticasChagas CardiomyopathyChronic DiseaseHumansImmunoenzyme TechniquesCardiomiopatia ChagásicaHumanosDoença crônicaTécnicas ImunoenzimáticasdiagnósticoChronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Testsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Referência para Doença de Chagas. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Saúde Pública. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.There is a significant heterogeneity in reported performance of serological assays for Chagas disease diagnosis. The conventional serology testing in laboratory diagnosis and in blood banks is unsatisfactory because of a high number of inconclusive and misclassified results. We aimed to assess the quality of four commercially available enzyme-linked immunosorbent assay tests for their ability to detect Trypanosoma cruzi antibodies in 685 sera samples. Cross-reactivity was assessed by using 748 sera from patients with unrelated diseases. Initially, we found that the reactivity index against T. cruzi antigen was statistically higher in sera from Chagas disease patients compared with those from non-chagasic patients, supporting the notion that all evaluated tests have a good discriminatory ability toward the diagnosis of T. cruzi infection in patients in the chronic phase of the disease. Although all tests were similarly sensitive for diagnosing T. cruzi infection, there were significant variations in terms of specificity and cross-reactivity among them. Indeed, we obtained divergent results when testing sera from patient with unrelated diseases, particularly leishmaniasis, with the levels of cross-reactivity being higher in tests using whole T. cruzi extracts compared with those using recombinant proteins. Our data suggest that all four tests may be used for the laboratory diagnosis and routine blood screening diagnose for Chagas disease. We also emphasize that, despite their general good performance, caution is needed when analyzing the results when these tests are performed in areas where other diseases, particularly leishmaniasis, are endemic.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20067/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL26976886 2016 san-chr.pdf26976886 2016 san-chr.pdfapplication/pdf577147https://www.arca.fiocruz.br/bitstream/icict/20067/2/26976886%202016%20san-chr.pdfd14929bf03e5d34bf6147d0b3bfbd735MD52TEXT26976886 2016 san-chr.pdf.txt26976886 2016 san-chr.pdf.txtExtracted texttext/plain34689https://www.arca.fiocruz.br/bitstream/icict/20067/3/26976886%202016%20san-chr.pdf.txt6abaa5f15f02b911db903fb97409e799MD53icict/200672018-08-15 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| dc.title.pt_BR.fl_str_mv |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| title |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| spellingShingle |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests Santos, Fred Luciano Neves Cardiomiopatia Chagásica Técnicas Imunoenzimáticas Chagas Cardiomyopathy Chronic Disease Humans Immunoenzyme Techniques Cardiomiopatia Chagásica Humanos Doença crônica Técnicas Imunoenzimáticas diagnóstico |
| title_short |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| title_full |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| title_fullStr |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| title_full_unstemmed |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| title_sort |
Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests |
| author |
Santos, Fred Luciano Neves |
| author_facet |
Santos, Fred Luciano Neves Souza, Wayner Vieira de Barros, Michelle da Silva Nakazawa, Mineo Krieger, Marco Aurélio Gomes, Yara de Miranda |
| author_role |
author |
| author2 |
Souza, Wayner Vieira de Barros, Michelle da Silva Nakazawa, Mineo Krieger, Marco Aurélio Gomes, Yara de Miranda |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Fred Luciano Neves Souza, Wayner Vieira de Barros, Michelle da Silva Nakazawa, Mineo Krieger, Marco Aurélio Gomes, Yara de Miranda |
| dc.subject.other.pt_BR.fl_str_mv |
Cardiomiopatia Chagásica Técnicas Imunoenzimáticas |
| topic |
Cardiomiopatia Chagásica Técnicas Imunoenzimáticas Chagas Cardiomyopathy Chronic Disease Humans Immunoenzyme Techniques Cardiomiopatia Chagásica Humanos Doença crônica Técnicas Imunoenzimáticas diagnóstico |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas Cardiomyopathy Chronic Disease Humans Immunoenzyme Techniques |
| dc.subject.decs.pt_BR.fl_str_mv |
Cardiomiopatia Chagásica Humanos Doença crônica Técnicas Imunoenzimáticas diagnóstico |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Referência para Doença de Chagas. Recife, PE, Brasil. |
| publishDate |
2016 |
| dc.date.issued.fl_str_mv |
2016 |
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2017-07-13T12:29:32Z |
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2017-07-13T12:29:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SANTOS, F. L. N. et al. Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests. The American Journal of Tropical Medicine and Hygiene, v. 94, n. 5, p. 1034–1039, 4 maio 2016. DOI 10.4269/ajtmh.15-0820. |
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https://www.arca.fiocruz.br/handle/icict/20067 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1476-1645 |
| dc.identifier.doi.none.fl_str_mv |
10.4269/ajtmh.15-0820 |
| identifier_str_mv |
SANTOS, F. L. N. et al. Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests. The American Journal of Tropical Medicine and Hygiene, v. 94, n. 5, p. 1034–1039, 4 maio 2016. DOI 10.4269/ajtmh.15-0820. 1476-1645 10.4269/ajtmh.15-0820 |
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