Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine

Detalhes bibliográficos
Autor(a) principal: Almeida-Paes, Rodrigo
Data de Publicação: 2016
Outros Autores: Figueiredo-Carvalho, Maria Helena Galdino, Brito-Santos, Fábio, Almeida-Silva, Fernando, Oliveira, Manoel Marques Evangelista, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31673
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Almeida-Paes, RodrigoFigueiredo-Carvalho, Maria Helena GaldinoBrito-Santos, FábioAlmeida-Silva, FernandoOliveira, Manoel Marques EvangelistaZancopé-Oliveira, Rosely Maria2019-02-15T13:24:17Z2019-02-15T13:24:17Z2016ALMEIDA-PAES, Rodrigo et al. Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine. Plos one, p. 1-13, Mar. 31 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/3167310.1371/journal.pone.01527961932-6203engPublic Library of ScienceMelanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Terbinafine is a recommended therapeutic alternative for patients with sporotrichosis who cannot use itraconazole due to drug interactions or side effects. Melanins are involved in resistance to antifungal drugs and Sporothrix species produce three different types of melanin. Therefore, in this study we evaluated whether Sporothrix melanins impact the efficacy of antifungal drugs. Minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC) of two Sporothrix brasiliensis and four Sporothrix schenckii strains grown in the presence of the melanin precursors L-DOPA and L-tyrosine were similar to the MIC determined by the CLSI standard protocol for S. schenckii susceptibility to amphotericin B, ketoconazole, itraconazole or terbinafine. When MICs were determined in the presence of inhibitors to three pathways of melanin synthesis, we observed, in four strains, an increase in terbinafine susceptibility in the presence of tricyclazole, a DHN-melanin inhibitor. In addition, one S. schenckii strain grown in the presence of L-DOPA had a higher MFC value when compared to the control. Growth curves in presence of 2×MIC concentrations of terbinafine showed that pyomelanin and, to a lesser extent, eumelanin were able to protect the fungi against the fungicidal effect of this antifungal drug. Our results suggest that melanin protects the major pathogenic species of the Sporothrix complex from the effects of terbinafine and that the development of new antifungal drugs targeting melanin synthesis may improve sporotrichosis therapies.Sporothrix brasiliensisSporothrix schenckiiTerbinafineAntifungal drugsMelaninsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31673/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Almeida-Paes_Rodrigo _etal_INI_2016.pdfve_Almeida-Paes_Rodrigo _etal_INI_2016.pdfapplication/pdf952268https://www.arca.fiocruz.br/bitstream/icict/31673/2/ve_Almeida-Paes_Rodrigo%20_etal_INI_2016.pdf08aab92130361de68f6f82001812a154MD52TEXTve_Almeida-Paes_Rodrigo _etal_INI_2016.pdf.txtve_Almeida-Paes_Rodrigo _etal_INI_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
title Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
spellingShingle Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
Almeida-Paes, Rodrigo
Sporothrix brasiliensis
Sporothrix schenckii
Terbinafine
Antifungal drugs
Melanins
title_short Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
title_full Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
title_fullStr Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
title_full_unstemmed Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
title_sort Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine
author Almeida-Paes, Rodrigo
author_facet Almeida-Paes, Rodrigo
Figueiredo-Carvalho, Maria Helena Galdino
Brito-Santos, Fábio
Almeida-Silva, Fernando
Oliveira, Manoel Marques Evangelista
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Figueiredo-Carvalho, Maria Helena Galdino
Brito-Santos, Fábio
Almeida-Silva, Fernando
Oliveira, Manoel Marques Evangelista
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida-Paes, Rodrigo
Figueiredo-Carvalho, Maria Helena Galdino
Brito-Santos, Fábio
Almeida-Silva, Fernando
Oliveira, Manoel Marques Evangelista
Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Sporothrix brasiliensis
Sporothrix schenckii
Terbinafine
Antifungal drugs
Melanins
topic Sporothrix brasiliensis
Sporothrix schenckii
Terbinafine
Antifungal drugs
Melanins
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-02-15T13:24:17Z
dc.date.available.fl_str_mv 2019-02-15T13:24:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA-PAES, Rodrigo et al. Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine. Plos one, p. 1-13, Mar. 31 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31673
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0152796
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv ALMEIDA-PAES, Rodrigo et al. Melanins protect Sporothrix brasiliensis and Sporothrix schenckii from the antifungal effects of Terbinafine. Plos one, p. 1-13, Mar. 31 2016.
1932-6203
10.1371/journal.pone.0152796
url https://www.arca.fiocruz.br/handle/icict/31673
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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