mRNA localization mechanisms in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Alves, Lysangela Ronalte
Data de Publicação: 2013
Outros Autores: Guerra‑Slompo, Eloise Pavão, Oliveira, Arthur V. de, Malgarin, Juliane Soldi, Goldenberg, Samuel, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15847
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Oswaldo Cruz
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spelling Alves, Lysangela RonalteGuerra‑Slompo, Eloise PavãoOliveira, Arthur V. deMalgarin, Juliane SoldiGoldenberg, SamuelDallagiovanna, Bruno2016-09-19T17:37:15Z2016-09-19T17:37:15Z2013ALVES, Lysangela Ronalte et al. mRNA localization mechanisms in Trypanosoma cruzi. PLoS ONE, v. 8, n. 12, p. 1-10, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/1584710.1371/journal.pone.0081375Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Oswaldo CruzFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Asymmetric mRNA localization is a sophisticated tool for regulating and optimizing protein synthesis and maintaining cell polarity. Molecular mechanisms involved in the regulated localization of transcripts are widespread in higher eukaryotes and fungi, but not in protozoa. Trypanosomes are ancient eukaryotes that branched off early in eukaryote evolution. We hypothesized that these organisms would have basic mechanisms of mRNA localization. FISH assays with probes against transcripts coding for proteins with restricted distributions showed a discrete localization of the mRNAs in the cytoplasm. Moreover, cruzipain mRNA was found inside reservosomes suggesting new unexpected functions for this vacuolar organelle. Individual mRNAs were also mobilized to RNA granules in response to nutritional stress. The cytoplasmic distribution of these transcripts changed with cell differentiation, suggesting that localization mechanisms might be involved in the regulation of stage-specific protein expression. Transfection assays with reporter genes showed that, as in higher eukaryotes, 39UTRs were responsible for guiding mRNAs to their final location. Our results strongly suggest that Trypanosoma cruzi have a core, basic mechanism of mRNA localization. This kind of controlled mRNA transport is ancient, dating back to early eukaryote evolution.porM. 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dc.title.pt_BR.fl_str_mv mRNA localization mechanisms in Trypanosoma cruzi
title mRNA localization mechanisms in Trypanosoma cruzi
spellingShingle mRNA localization mechanisms in Trypanosoma cruzi
Alves, Lysangela Ronalte
mRNA
Trypanosoma cruzi
RNA Mensageiro
Eucariotos
title_short mRNA localization mechanisms in Trypanosoma cruzi
title_full mRNA localization mechanisms in Trypanosoma cruzi
title_fullStr mRNA localization mechanisms in Trypanosoma cruzi
title_full_unstemmed mRNA localization mechanisms in Trypanosoma cruzi
title_sort mRNA localization mechanisms in Trypanosoma cruzi
author Alves, Lysangela Ronalte
author_facet Alves, Lysangela Ronalte
Guerra‑Slompo, Eloise Pavão
Oliveira, Arthur V. de
Malgarin, Juliane Soldi
Goldenberg, Samuel
Dallagiovanna, Bruno
author_role author
author2 Guerra‑Slompo, Eloise Pavão
Oliveira, Arthur V. de
Malgarin, Juliane Soldi
Goldenberg, Samuel
Dallagiovanna, Bruno
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Lysangela Ronalte
Guerra‑Slompo, Eloise Pavão
Oliveira, Arthur V. de
Malgarin, Juliane Soldi
Goldenberg, Samuel
Dallagiovanna, Bruno
dc.subject.en.pt_BR.fl_str_mv mRNA
Trypanosoma cruzi
topic mRNA
Trypanosoma cruzi
RNA Mensageiro
Eucariotos
dc.subject.decs.pt_BR.fl_str_mv RNA Mensageiro
Eucariotos
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Oswaldo Cruz
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-09-19T17:37:15Z
dc.date.available.fl_str_mv 2016-09-19T17:37:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Lysangela Ronalte et al. mRNA localization mechanisms in Trypanosoma cruzi. PLoS ONE, v. 8, n. 12, p. 1-10, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15847
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0081375
identifier_str_mv ALVES, Lysangela Ronalte et al. mRNA localization mechanisms in Trypanosoma cruzi. PLoS ONE, v. 8, n. 12, p. 1-10, 2013.
1932-6203
10.1371/journal.pone.0081375
url https://www.arca.fiocruz.br/handle/icict/15847
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv M. Carolina Elias
publisher.none.fl_str_mv M. Carolina Elias
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15847/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/15847/3/mRNA%20Localization%20Mechanisms%20in%20Trypanosoma%20cruzi.PDF.txt
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