A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57717 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. |
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Sena, Edlainne Pinheiro FerreiraHardoim, Daiana de JesusCardoso, Flavia de Oliveirad’Escoffier, Luiz NeySoares, Isabela FerreiraCarvalho, João Pedro Rangel da SilvaAngnes, Ricardo AlmirFragoso, Stenio PerdigãoAlves, Carlos RobertoDe Simone, Salvatore GiovanniLima Junior, Josué da CostaBertho, Alvaro LuizValle, Tânia Zaverucha doSilva, Franklin Souza daCalabrese, Kátia da Silva2023-04-08T16:00:00Z2023-04-08T16:00:00Z2023SENA, Edlainne Pinheiro Ferreira et al.A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I. International Journal of Molecular Sciences, v. 24, 5972, p. 1-15, Mar. 2023.1422-0067https://www.arca.fiocruz.br/handle/icict/5771710.3390/ ijms24065972engMDPILeishmaniose cutâneaEpítopos TPeptídeosVacinaBioinformáticaHLAH2DbMHC ITetrâmeroCutaneous leishmaniasisTepitopesPeptidesVaccineBioinformaticsHLAH2DbMHC ITetramerA new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class Iinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Instituto de Biologia Molecular do Paraná. Laboratório de Síntese Química. Curitiba. PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Programa de Pós-Graduação em Ciência e Biotecnologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu, Dom Rodrigo, Nova Iguaçu. Faculdade de Biologia e Ciências da Saúde. Nova Iguaçu,, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Leishmaniasis represents a complex of diseases with a broad clinical spectrum and epidemiological diversity, considered a major public health problem. Although there is treatment, there are still no vaccines for cutaneous leishmaniasis. Because Leishmania spp. is an intracellular protozoan with several escape mechanisms, a vaccine must provoke cellular and humoral immune responses. Previously, we identified the Leishmania homolog of receptors for activated C kinase (LACK) and phosphoenolpyruvate carboxykinase (PEPCK) proteins as strong immunogens and candidates for the development of a vaccine strategy. The present work focuses on the in silico prediction and characterization of antigenic epitopes that might interact with mice or human major histocompatibility complex class I. After immunogenicity prediction on the Immune Epitope Database (IEDB) and the Database of MHC Ligands and Peptide Motifs (SYFPEITHI), 26 peptides were selected for interaction assays with infected mouse lymphocytes by flow cytometry and ELISpot. This strategy identified nine antigenic peptides (pL1-H2, pPL3-H2, pL10-HLA, pP13-H2, pP14-H2, pP15-H2, pP16-H2, pP17-H2, pP18-H2, pP26-HLA), which are strong candidates for developing a peptide vaccine against leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57717/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIsabelaSoares_StenioCardoso_etal_IOC_2023.pdfIsabelaSoares_StenioCardoso_etal_IOC_2023.pdfapplication/pdf1524240https://www.arca.fiocruz.br/bitstream/icict/57717/2/IsabelaSoares_StenioCardoso_etal_IOC_2023.pdf5b0a6dae2c0eb38cf5838da16ee6b3cbMD52icict/577172023-09-14 15:46:53.322oai:www.arca.fiocruz.br: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ório 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| dc.title.en_US.fl_str_mv |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| title |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| spellingShingle |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I Sena, Edlainne Pinheiro Ferreira Leishmaniose cutânea Epítopos T Peptídeos Vacina Bioinformática HLA H2Db MHC I Tetrâmero Cutaneous leishmaniasis Tepitopes Peptides Vaccine Bioinformatics HLA H2Db MHC I Tetramer |
| title_short |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| title_full |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| title_fullStr |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| title_full_unstemmed |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| title_sort |
A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I |
| author |
Sena, Edlainne Pinheiro Ferreira |
| author_facet |
Sena, Edlainne Pinheiro Ferreira Hardoim, Daiana de Jesus Cardoso, Flavia de Oliveira d’Escoffier, Luiz Ney Soares, Isabela Ferreira Carvalho, João Pedro Rangel da Silva Angnes, Ricardo Almir Fragoso, Stenio Perdigão Alves, Carlos Roberto De Simone, Salvatore Giovanni Lima Junior, Josué da Costa Bertho, Alvaro Luiz Valle, Tânia Zaverucha do Silva, Franklin Souza da Calabrese, Kátia da Silva |
| author_role |
author |
| author2 |
Hardoim, Daiana de Jesus Cardoso, Flavia de Oliveira d’Escoffier, Luiz Ney Soares, Isabela Ferreira Carvalho, João Pedro Rangel da Silva Angnes, Ricardo Almir Fragoso, Stenio Perdigão Alves, Carlos Roberto De Simone, Salvatore Giovanni Lima Junior, Josué da Costa Bertho, Alvaro Luiz Valle, Tânia Zaverucha do Silva, Franklin Souza da Calabrese, Kátia da Silva |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sena, Edlainne Pinheiro Ferreira Hardoim, Daiana de Jesus Cardoso, Flavia de Oliveira d’Escoffier, Luiz Ney Soares, Isabela Ferreira Carvalho, João Pedro Rangel da Silva Angnes, Ricardo Almir Fragoso, Stenio Perdigão Alves, Carlos Roberto De Simone, Salvatore Giovanni Lima Junior, Josué da Costa Bertho, Alvaro Luiz Valle, Tânia Zaverucha do Silva, Franklin Souza da Calabrese, Kátia da Silva |
| dc.subject.other.en_US.fl_str_mv |
Leishmaniose cutânea Epítopos T Peptídeos Vacina Bioinformática HLA H2Db MHC I Tetrâmero |
| topic |
Leishmaniose cutânea Epítopos T Peptídeos Vacina Bioinformática HLA H2Db MHC I Tetrâmero Cutaneous leishmaniasis Tepitopes Peptides Vaccine Bioinformatics HLA H2Db MHC I Tetramer |
| dc.subject.en.en_US.fl_str_mv |
Cutaneous leishmaniasis Tepitopes Peptides Vaccine Bioinformatics HLA H2Db MHC I Tetramer |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. |
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2023 |
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2023-04-08T16:00:00Z |
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2023-04-08T16:00:00Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SENA, Edlainne Pinheiro Ferreira et al.A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I. International Journal of Molecular Sciences, v. 24, 5972, p. 1-15, Mar. 2023. |
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https://www.arca.fiocruz.br/handle/icict/57717 |
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1422-0067 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ ijms24065972 |
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SENA, Edlainne Pinheiro Ferreira et al.A new strategy for mapping epitopes of LACK and PEPCK proteins of Leishmania amazonensis specific for major histocompatibility complex class I. International Journal of Molecular Sciences, v. 24, 5972, p. 1-15, Mar. 2023. 1422-0067 10.3390/ ijms24065972 |
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