Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19508 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
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Xavier-Neto, JoseCarvalho, MuriloPascoalino, Bruno Dos SantosCardoso, Alisson CamposCosta, Ângela Maria SousaPereira, Ana Helena MacedoSantos, Luana NunesSaito, ÂngelaMarques, Rafael EliasSmetana, Juliana Helena CostaConsonni, Silvio RobertoBandeira, CarlaCosta, Vivian VasconcelosBajgelman, Marcio ChaimOliveira, Paulo Sérgio Lopes deCordeiro, Marli TenorioGonzales Gil, Laura Helena VegaPauletti, Bianca AlvesGranato, Daniela CamposPaes Leme, Adriana FrancoFreitas-Junior, LucioHolanda de Freitas, Carolina Borsoi MoraesTeixeira, Mauro MartinsBevilacqua, EstelaFranchini, Kleber2017-06-26T14:23:58Z2017-06-26T14:23:58Z2017XAVIER-NETO, J. et al. Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: A developmental study. PLoS neglected tropical diseases, v. 11, n. 2, p. e0005363, fev. 2017.1935-2735https://www.arca.fiocruz.br/handle/icict/1950810.1371/journal.pntd.0005363porZika virusModelos animaisArtrogriposeZika virusModels, AnimalArthrogryposisZika virusModelos AnimaisArtrogriposeHydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilThe teratogenic mechanisms triggered by ZIKV are still obscure due to the lack of a suitable animal model. Here we present a mouse model of developmental disruption induced by ZIKV hematogenic infection. The model utilizes immunocompetent animals from wild-type FVB/NJ and C57BL/6J strains, providing a better analogy to the human condition than approaches involving immunodeficient, genetically modified animals, or direct ZIKV injection into the brain. When injected via the jugular vein into the blood of pregnant females harboring conceptuses from early gastrulation to organogenesis stages, akin to the human second and fifth week of pregnancy, ZIKV infects maternal tissues, placentas and embryos/fetuses. Early exposure to ZIKV at developmental day 5 (second week in humans) produced complex manifestations of anterior and posterior dysraphia and hydrocephalus, as well as severe malformations and delayed development in 10.5 days post-coitum (dpc) embryos. Exposure to the virus at 7.5-9.5 dpc induces intra-amniotic hemorrhage, widespread edema, and vascular rarefaction, often prominent in the cephalic region. At these stages, most affected embryos/fetuses displayed gross malformations and/or intrauterine growth restriction (IUGR), rather than isolated microcephaly. Disrupted conceptuses failed to achieve normal developmental landmarks and died in utero. Importantly, this is the only model so far to display dysraphia and hydrocephalus, the harbinger of microcephaly in humans, as well as arthrogryposis, a set of abnormal joint postures observed in the human setting. Late exposure to ZIKV at 12.5 dpc failed to produce noticeable malformations. We have thus characterized a developmental window of opportunity for ZIKV-induced teratogenesis encompassing early gastrulation, neurulation and early organogenesis stages. This should not, however, be interpreted as evidence for any safe developmental windows for ZIKV exposure. Late developmental abnormalities correlated with damage to the placenta, particularly to the labyrinthine layer, suggesting that circulatory changes are integral to the altered phenotypes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19508/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL28231241 2017 xav-hyd.oa.pdf28231241 2017 xav-hyd.oa.pdfapplication/pdf3679006https://www.arca.fiocruz.br/bitstream/icict/19508/2/28231241%202017%20xav-hyd.oa.pdff24ae366311a286279f0f0d3a9c930ebMD52TEXT28231241 2017 xav-hyd.oa.pdf.txt28231241 2017 xav-hyd.oa.pdf.txtExtracted texttext/plain92117https://www.arca.fiocruz.br/bitstream/icict/19508/3/28231241%202017%20xav-hyd.oa.pdf.txte527f66e1f79998268704bae3b5e377aMD53icict/195082018-08-15 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| dc.title.pt_BR.fl_str_mv |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| title |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| spellingShingle |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study Xavier-Neto, Jose Zika virus Modelos animais Artrogripose Zika virus Models, Animal Arthrogryposis Zika virus Modelos Animais Artrogripose |
| title_short |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| title_full |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| title_fullStr |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| title_full_unstemmed |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| title_sort |
Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: a developmental study |
| author |
Xavier-Neto, Jose |
| author_facet |
Xavier-Neto, Jose Carvalho, Murilo Pascoalino, Bruno Dos Santos Cardoso, Alisson Campos Costa, Ângela Maria Sousa Pereira, Ana Helena Macedo Santos, Luana Nunes Saito, Ângela Marques, Rafael Elias Smetana, Juliana Helena Costa Consonni, Silvio Roberto Bandeira, Carla Costa, Vivian Vasconcelos Bajgelman, Marcio Chaim Oliveira, Paulo Sérgio Lopes de Cordeiro, Marli Tenorio Gonzales Gil, Laura Helena Vega Pauletti, Bianca Alves Granato, Daniela Campos Paes Leme, Adriana Franco Freitas-Junior, Lucio Holanda de Freitas, Carolina Borsoi Moraes Teixeira, Mauro Martins Bevilacqua, Estela Franchini, Kleber |
| author_role |
author |
| author2 |
Carvalho, Murilo Pascoalino, Bruno Dos Santos Cardoso, Alisson Campos Costa, Ângela Maria Sousa Pereira, Ana Helena Macedo Santos, Luana Nunes Saito, Ângela Marques, Rafael Elias Smetana, Juliana Helena Costa Consonni, Silvio Roberto Bandeira, Carla Costa, Vivian Vasconcelos Bajgelman, Marcio Chaim Oliveira, Paulo Sérgio Lopes de Cordeiro, Marli Tenorio Gonzales Gil, Laura Helena Vega Pauletti, Bianca Alves Granato, Daniela Campos Paes Leme, Adriana Franco Freitas-Junior, Lucio Holanda de Freitas, Carolina Borsoi Moraes Teixeira, Mauro Martins Bevilacqua, Estela Franchini, Kleber |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Xavier-Neto, Jose Carvalho, Murilo Pascoalino, Bruno Dos Santos Cardoso, Alisson Campos Costa, Ângela Maria Sousa Pereira, Ana Helena Macedo Santos, Luana Nunes Saito, Ângela Marques, Rafael Elias Smetana, Juliana Helena Costa Consonni, Silvio Roberto Bandeira, Carla Costa, Vivian Vasconcelos Bajgelman, Marcio Chaim Oliveira, Paulo Sérgio Lopes de Cordeiro, Marli Tenorio Gonzales Gil, Laura Helena Vega Pauletti, Bianca Alves Granato, Daniela Campos Paes Leme, Adriana Franco Freitas-Junior, Lucio Holanda de Freitas, Carolina Borsoi Moraes Teixeira, Mauro Martins Bevilacqua, Estela Franchini, Kleber |
| dc.subject.other.pt_BR.fl_str_mv |
Zika virus Modelos animais Artrogripose |
| topic |
Zika virus Modelos animais Artrogripose Zika virus Models, Animal Arthrogryposis Zika virus Modelos Animais Artrogripose |
| dc.subject.en.pt_BR.fl_str_mv |
Zika virus Models, Animal Arthrogryposis |
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Zika virus Modelos Animais Artrogripose |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
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2017 |
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2017-06-26T14:23:58Z |
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2017-06-26T14:23:58Z |
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2017 |
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info:eu-repo/semantics/article |
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XAVIER-NETO, J. et al. Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: A developmental study. PLoS neglected tropical diseases, v. 11, n. 2, p. e0005363, fev. 2017. |
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https://www.arca.fiocruz.br/handle/icict/19508 |
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1935-2735 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0005363 |
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XAVIER-NETO, J. et al. Hydrocephalus and arthrogryposis in an immunocompetent mouse model of ZIKA teratogeny: A developmental study. PLoS neglected tropical diseases, v. 11, n. 2, p. e0005363, fev. 2017. 1935-2735 10.1371/journal.pntd.0005363 |
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