Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)

Detalhes bibliográficos
Autor(a) principal: Bayona-Serrano, J. D.
Data de Publicação: 2023
Outros Autores: Grazziotin, F. G., Salazar-Valenzuela, D., Valente, R. H., Nachtigall, P. G., Colombini, M., Moura da Silva, A. M., Junqueira-de-Azevedo, I. L. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59182
Resumo: Running title: Evolution of the PLA2 scaffold within rear-fanged snakes
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spelling Bayona-Serrano, J. D.Grazziotin, F. G.Salazar-Valenzuela, D.Valente, R. H.Nachtigall, P. G.Colombini, M.Moura da Silva, A. M.Junqueira-de-Azevedo, I. L. M.2023-06-26T18:03:14Z2023-06-26T18:03:14Z2023BAYONA-SERRANO, J. D. et al. Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae).https://www.arca.fiocruz.br/handle/icict/59182Running title: Evolution of the PLA2 scaffold within rear-fanged snakesInstituto Butantan. Laboratório de Toxinologia Aplicada (LETA), São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Coleções Zoológicas (LECZ). São Paulo, SP, Brasil.Universidad Indoamérica, Machala y Sabanilla 170301. Facultad de Ciencias del Medio Ambiente. Ingeniería en Biodiversidad y Recursos Genéticos. Centro de Investigación de la Biodiversidad y Cambio Climático (BioCamb). Quito, Ecuador.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Laboratório de Toxinologia Aplicada (LETA), São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Toxinologia Aplicada (LETA), São Paulo, SP, Brasil / Center of Toxins, Immune-Response and Cell Signaling (CeTICS), São Paulo, SP, Brazil.Snake venom harbors a wide and diverse array of enzymatic and nonenzymatic toxic 2 components, allowing them to exert myriad effects on their prey. However, they appear to 3 trend toward a few optimal compositional scaffolds, dominated by four major toxin 4 classes: SVMPs, SVSPs, 3FTxs and PLA2s. Nevertheless, the latter appears to be 5 restricted to vipers and elapids, as it has never been reported as a major venom component 6 in rear-fanged species. Here, by investigating the original transcriptomes from 19 species 7 distributed in eight genera from the Pseudoboini tribe (Dipsadidae: Xenodontinae) and 8 screening among seven additional tribes of Dipsadidae and three additional families of 9 advanced snakes, we discovered that a novel type of venom, PLA2, resembling a PLA2-10 IIE, has been recruited to the venom of some species of the Pseudoboini tribe, where it is a major component. Proteomic and functional analyses of these venoms further indicate 12 that these PLA2s play a relevant role in the venoms from this tribe. Moreover, we 13 reconstructed the phylogeny of PLA2s across different snake groups and show that 14 different types of these toxins have been recruited in at least five independent events in 15 caenophidian snakes. Additionally, we present the first compositional profiling of 16 Pseudoboini venoms. Our results demonstrate how relevant phenotypic traits are 17 convergently recruited by different means and from homologous and nonhomologous 18 genes in phylogenetically and ecologically divergent snake groups, possibly optimizing venom composition to overcome diverse adaptative landscapes.engOxford University PressFosfolipases A2Evolução da família de proteínasCooptação eneVeneno de cobraDipsadidaePhospholipases A2Protein family evolutionGene co-optionSnake venomDipsadidaeIndependent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRichardValente_etal_IOC_2023.pdfRichardValente_etal_IOC_2023.pdfapplication/pdf1661081https://www.arca.fiocruz.br/bitstream/icict/59182/2/RichardValente_etal_IOC_2023.pdfa2db6b2a1de881b3081f56b399ab7355MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59182/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/591822023-06-26 15:03:15.148oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-26T18:03:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
title Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
spellingShingle Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
Bayona-Serrano, J. D.
Fosfolipases A2
Evolução da família de proteínas
Cooptação ene
Veneno de cobra
Dipsadidae
Phospholipases A2
Protein family evolution
Gene co-option
Snake venom
Dipsadidae
title_short Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
title_full Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
title_fullStr Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
title_full_unstemmed Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
title_sort Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae)
author Bayona-Serrano, J. D.
author_facet Bayona-Serrano, J. D.
Grazziotin, F. G.
Salazar-Valenzuela, D.
Valente, R. H.
Nachtigall, P. G.
Colombini, M.
Moura da Silva, A. M.
Junqueira-de-Azevedo, I. L. M.
author_role author
author2 Grazziotin, F. G.
Salazar-Valenzuela, D.
Valente, R. H.
Nachtigall, P. G.
Colombini, M.
Moura da Silva, A. M.
Junqueira-de-Azevedo, I. L. M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bayona-Serrano, J. D.
Grazziotin, F. G.
Salazar-Valenzuela, D.
Valente, R. H.
Nachtigall, P. G.
Colombini, M.
Moura da Silva, A. M.
Junqueira-de-Azevedo, I. L. M.
dc.subject.other.en_US.fl_str_mv Fosfolipases A2
Evolução da família de proteínas
Cooptação ene
Veneno de cobra
Dipsadidae
topic Fosfolipases A2
Evolução da família de proteínas
Cooptação ene
Veneno de cobra
Dipsadidae
Phospholipases A2
Protein family evolution
Gene co-option
Snake venom
Dipsadidae
dc.subject.en.en_US.fl_str_mv Phospholipases A2
Protein family evolution
Gene co-option
Snake venom
Dipsadidae
description Running title: Evolution of the PLA2 scaffold within rear-fanged snakes
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-06-26T18:03:14Z
dc.date.available.fl_str_mv 2023-06-26T18:03:14Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BAYONA-SERRANO, J. D. et al. Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae).
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59182
identifier_str_mv BAYONA-SERRANO, J. D. et al. Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae).
url https://www.arca.fiocruz.br/handle/icict/59182
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59182/2/RichardValente_etal_IOC_2023.pdf
https://www.arca.fiocruz.br/bitstream/icict/59182/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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