Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B

Detalhes bibliográficos
Autor(a) principal: Barral-Veloso, Laura
Data de Publicação: 2020
Outros Autores: Melo, Barbara Cristina de Albuquerque, Souza, Raquel Santos de, Cysne-Finkelstein, Léa, Silva, Franklin Souza, Santos, Fernanda Nunes, Sousa, Joel Fontes de, Alves, Carlos Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42273
Resumo: Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, Brasil
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spelling Barral-Veloso, LauraMelo, Barbara Cristina de AlbuquerqueSouza, Raquel Santos deCysne-Finkelstein, LéaSilva, Franklin SouzaSantos, Fernanda NunesSousa, Joel Fontes deAlves, Carlos Roberto2020-07-15T21:22:06Z2020-07-15T21:22:06Z2020BARRAL-VELOSO, Laura et al. Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B. Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 201-207, 2020.1413-8670https://www.arca.fiocruz.br/handle/icict/4227310.1016/j.bjid.2020.04.0061678-4391engSociedade Brasileira de InfectologiaLeishmaniose caninaCisteína proteinase BLeishmania spp.Ensaios imunosorbentes ligados à enzimaProteaseProteaseCysteine proteinase BLeishmania spp.Enzyme-linked immunosorbent assaysCanine leishmaniasisReactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase Binfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, BrasilFundac¸ão Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia.. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Laboratório de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Centro de Controle de Zoonoses. Montes Claros, MG, Brasil.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, BrasilCysteine proteinases are well-known virulence factors of Leishmania spp. with demonstrated actions in both experimental mouse infection and human infection. However, studies on these enzymes in canine leishmaniasis are scarce. Here, we show, for the first time, the reactivity of sera from dogs living in an endemic area to a recombinant protein from the COOH-terminal region of cysteine B protease. In this work, enzyme-linked immunosorbent assays were performed using a 14kDa rcyspep protein obtained through a pET28-a expression system in Escherichia coli. First, 96-well plates were coated with rcyspep (500ng/well) and incubated with sera from dogs (1:100). Subsequently, IgG antibody detection was performed using rabbit anti-dog IgG antibodies conjugated with peroxidase. Sera from dogs (n=114), including suspect (n=30) and positive (n=50) dogs from a leishmaniasis-endemic area and dogs from a nonendemic area, (n=34), negative for leishmaniasis, were assessed. The results showed that sera from the suspect (42%) and positive (68%) groups responded differently to the antigen titers tested above the cut-off (Optical Density=0.166). This finding suggests that the immune response detected against cyspep may be related to clinical disorders present in these animals. Collectively, the data gathered here suggest that cyspep can sensitize the immune systems of dogs from a leishmaniasis-endemic area to elicit a humoral response, an immunological parameter indicating the contribution of this protein in host-parasite interaction.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42273/1/license.txt5a560609d32a3863062d77ff32785d58MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
title Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
spellingShingle Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
Barral-Veloso, Laura
Leishmaniose canina
Cisteína proteinase B
Leishmania spp.
Ensaios imunosorbentes ligados à enzima
Protease
Protease
Cysteine proteinase B
Leishmania spp.
Enzyme-linked immunosorbent assays
Canine leishmaniasis
title_short Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
title_full Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
title_fullStr Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
title_full_unstemmed Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
title_sort Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B
author Barral-Veloso, Laura
author_facet Barral-Veloso, Laura
Melo, Barbara Cristina de Albuquerque
Souza, Raquel Santos de
Cysne-Finkelstein, Léa
Silva, Franklin Souza
Santos, Fernanda Nunes
Sousa, Joel Fontes de
Alves, Carlos Roberto
author_role author
author2 Melo, Barbara Cristina de Albuquerque
Souza, Raquel Santos de
Cysne-Finkelstein, Léa
Silva, Franklin Souza
Santos, Fernanda Nunes
Sousa, Joel Fontes de
Alves, Carlos Roberto
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barral-Veloso, Laura
Melo, Barbara Cristina de Albuquerque
Souza, Raquel Santos de
Cysne-Finkelstein, Léa
Silva, Franklin Souza
Santos, Fernanda Nunes
Sousa, Joel Fontes de
Alves, Carlos Roberto
dc.subject.other.pt_BR.fl_str_mv Leishmaniose canina
Cisteína proteinase B
Leishmania spp.
Ensaios imunosorbentes ligados à enzima
Protease
topic Leishmaniose canina
Cisteína proteinase B
Leishmania spp.
Ensaios imunosorbentes ligados à enzima
Protease
Protease
Cysteine proteinase B
Leishmania spp.
Enzyme-linked immunosorbent assays
Canine leishmaniasis
dc.subject.en.pt_BR.fl_str_mv Protease
Cysteine proteinase B
Leishmania spp.
Enzyme-linked immunosorbent assays
Canine leishmaniasis
description Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, Brasil
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-07-15T21:22:06Z
dc.date.available.fl_str_mv 2020-07-15T21:22:06Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARRAL-VELOSO, Laura et al. Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B. Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 201-207, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42273
dc.identifier.issn.pt_BR.fl_str_mv 1413-8670
dc.identifier.doi.none.fl_str_mv 10.1016/j.bjid.2020.04.006
dc.identifier.eissn.none.fl_str_mv 1678-4391
identifier_str_mv BARRAL-VELOSO, Laura et al. Reactivity of sera from dogs living in a leishmaniasis-endemic area to the COOH-terminal region of cysteine proteinase B. Brazilian Journal of Infectious Diseases, v. 24, n. 3, p. 201-207, 2020.
1413-8670
10.1016/j.bjid.2020.04.006
1678-4391
url https://www.arca.fiocruz.br/handle/icict/42273
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Infectologia
publisher.none.fl_str_mv Sociedade Brasileira de Infectologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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