Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
| Main Author: | |
|---|---|
| Publication Date: | 2014 |
| Other Authors: | , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| Download full: | https://www.arca.fiocruz.br/handle/icict/16050 |
Summary: | Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR. |
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Inoue, Alexandre HaruoSerpeloni, MarianaHiraiwa, Priscila MazzocchiYamada-Ogatta, Sueli FumieMuniz, João Renato CarvalhoMotta, Maria Cristina MachadoVidal, Newton MedeirosGoldenberg, SamuelÁvila, Andréa Rodrigues2016-10-03T16:43:16Z2016-10-03T16:43:16Z2014INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, 2014.1932-6203https://www.arca.fiocruz.br/handle/icict/1605010.1371/journal.pone.0109521Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Paraná. Laboratório de Biologia Celular e Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Departamento de Biologia Celular e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / National Institutes of Health. National Library of Medicine. National Center for Biotechnology Information. Bethesda, Maryland, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor.engIratxe PueblaIdentification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRNA helicaseTrypanosomesNucleocytoplasmic shuttlingGene ExpressionExpressão GênicaTransporte Ativo do Núcleo CelularTrypanosomainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16050/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALIdentification of a novel nucleocytoplasmic shuttling RNA.pdfIdentification of a novel nucleocytoplasmic shuttling RNA.pdfapplication/pdf1447090https://www.arca.fiocruz.br/bitstream/icict/16050/2/Identification%20of%20a%20novel%20nucleocytoplasmic%20shuttling%20RNA.pdf503affa655f319218c00aae5792b6a1fMD52TEXTIdentification of a novel nucleocytoplasmic shuttling RNA.pdf.txtIdentification of a novel nucleocytoplasmic shuttling RNA.pdf.txtExtracted texttext/plain57852https://www.arca.fiocruz.br/bitstream/icict/16050/3/Identification%20of%20a%20novel%20nucleocytoplasmic%20shuttling%20RNA.pdf.txt63e40564bba6f06cce66751ca1f619eaMD53icict/160502022-10-20 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| dc.title.pt_BR.fl_str_mv |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| title |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| spellingShingle |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes Inoue, Alexandre Haruo RNA helicase Trypanosomes Nucleocytoplasmic shuttling Gene Expression Expressão Gênica Transporte Ativo do Núcleo Celular Trypanosoma |
| title_short |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| title_full |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| title_fullStr |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| title_full_unstemmed |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| title_sort |
Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes |
| author |
Inoue, Alexandre Haruo |
| author_facet |
Inoue, Alexandre Haruo Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues |
| author_role |
author |
| author2 |
Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Inoue, Alexandre Haruo Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues |
| dc.subject.en.pt_BR.fl_str_mv |
RNA helicase Trypanosomes Nucleocytoplasmic shuttling |
| topic |
RNA helicase Trypanosomes Nucleocytoplasmic shuttling Gene Expression Expressão Gênica Transporte Ativo do Núcleo Celular Trypanosoma |
| dc.subject.en.En.fl_str_mv |
Gene Expression |
| dc.subject.decs.pt_BR.fl_str_mv |
Expressão Gênica Transporte Ativo do Núcleo Celular Trypanosoma |
| description |
Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR. |
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2014 |
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2014 |
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2016-10-03T16:43:16Z |
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2016-10-03T16:43:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, 2014. |
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https://www.arca.fiocruz.br/handle/icict/16050 |
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1932-6203 |
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10.1371/journal.pone.0109521 |
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INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, 2014. 1932-6203 10.1371/journal.pone.0109521 |
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