Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis

Bibliographic Details
Main Author: Gesto, João Silveira Moledo
Publication Date: 2015
Other Authors: Rivas, Gustavo Bueno da Silva, Pavan, Marcio Galvão, Meireles Filho, Antonio Carlos Alves, Amoretty, Paulo Roberto de, Souza, Nataly Araújo de, Bruno, Rafaela Vieira, Peixoto, Alexandre Afranio
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/13094
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.
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spelling Gesto, João Silveira MoledoRivas, Gustavo Bueno da SilvaPavan, Marcio GalvãoMeireles Filho, Antonio Carlos AlvesAmoretty, Paulo Roberto deSouza, Nataly Araújo deBruno, Rafaela VieiraPeixoto, Alexandre Afranio2016-03-10T14:31:30Z2016-03-10T14:31:30Z2015GESTO, João Silveira Moledo; et al. Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis. Parasite & Vectors, v.8:505, 5p, 2015.1756-3305https://www.arca.fiocruz.br/handle/icict/1309410.1186/s13071-015-1117-6engBioMed CentralClocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.École Polytechnique Fédérale de Lausanne. School of Life Sciences. , Institute of Bioengineering. Laboratory of Systems Biology and Genetics. Lausanne, Switzerland.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM)/ CNPq. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM)/ CNPq. Rio de Janeiro, RJ, Brasil.Background: Behavior rhythms of insect vectors directly interfere with the dynamics of pathogen transmission to humans. The sand fly Lutzomyia longipalpis is the main vector of visceral leishmaniasis in America and concentrates its activity around dusk. Despite the accumulation of behavioral data, very little is known about the molecular bases of the clock mechanism in this species. This study aims to characterize, within an evolutionary perspective, two important circadian clock genes, Clock and vrille. Findings: We have cloned and isolated the coding sequence of L. longipalpis’ genes Clock and vrille. The former is structured in eight exons and encodes a protein of 696 amino acids, and the latter comprises three exons and translates to a protein of 469 amino acids. When compared to other insects’ orthologues, L. longipalpis CLOCK shows a high degree of conservation in the functional domains bHLH and PAS, but a much shorter glutamine-rich (poly-Q) C-terminal region. As for L. longipalpis VRILLE, a high degree of conservation was found in the bZIP domain. To support these observations and provide an elegant view of the evolution of both genes in insects, phylogenetic analyses based on maximum-likelihood and Bayesian inferences were performed, corroborating the previously known insect systematics. Conclusions: The isolation and phylogenetic analyses of Clock and vrille orthologues in L. longipalpis bring novel and important data to characterize this species’ circadian clock. Interestingly, the poly-Q shortening observed in CLOCK suggests that its transcription activity might be impaired and we speculate if this effect could be compensated by other clock factors such as CYCLE.Lutzomyia longipalpiCircadian clocksClock, vrillePAS domainbZIP domainbHLH domainVisceral leishmaniasisLeishmaniose VisceralRelógios Circadianosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13094/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoao_gesto_etal_IOC_2015.pdfjoao_gesto_etal_IOC_2015.pdfapplication/pdf553724https://www.arca.fiocruz.br/bitstream/icict/13094/2/joao_gesto_etal_IOC_2015.pdfb41592733dbfe464b0c6b7da57943612MD52TEXTjoao_gesto_etal_IOC_2015.pdf.txtjoao_gesto_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
title Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
spellingShingle Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
Gesto, João Silveira Moledo
Lutzomyia longipalpi
Circadian clocks
Clock, vrille
PAS domain
bZIP domain
bHLH domain
Visceral leishmaniasis
Leishmaniose Visceral
Relógios Circadianos
title_short Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
title_full Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
title_fullStr Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
title_full_unstemmed Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
title_sort Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis
author Gesto, João Silveira Moledo
author_facet Gesto, João Silveira Moledo
Rivas, Gustavo Bueno da Silva
Pavan, Marcio Galvão
Meireles Filho, Antonio Carlos Alves
Amoretty, Paulo Roberto de
Souza, Nataly Araújo de
Bruno, Rafaela Vieira
Peixoto, Alexandre Afranio
author_role author
author2 Rivas, Gustavo Bueno da Silva
Pavan, Marcio Galvão
Meireles Filho, Antonio Carlos Alves
Amoretty, Paulo Roberto de
Souza, Nataly Araújo de
Bruno, Rafaela Vieira
Peixoto, Alexandre Afranio
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gesto, João Silveira Moledo
Rivas, Gustavo Bueno da Silva
Pavan, Marcio Galvão
Meireles Filho, Antonio Carlos Alves
Amoretty, Paulo Roberto de
Souza, Nataly Araújo de
Bruno, Rafaela Vieira
Peixoto, Alexandre Afranio
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpi
Circadian clocks
Clock, vrille
PAS domain
bZIP domain
bHLH domain
Visceral leishmaniasis
topic Lutzomyia longipalpi
Circadian clocks
Clock, vrille
PAS domain
bZIP domain
bHLH domain
Visceral leishmaniasis
Leishmaniose Visceral
Relógios Circadianos
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose Visceral
Relógios Circadianos
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-10T14:31:30Z
dc.date.available.fl_str_mv 2016-03-10T14:31:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GESTO, João Silveira Moledo; et al. Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis. Parasite & Vectors, v.8:505, 5p, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13094
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-015-1117-6
identifier_str_mv GESTO, João Silveira Moledo; et al. Clocks do not tick in unison: isolation of Clock and vrille shed new light on the clockwork model of the sand fly Lutzomyia longipalpis. Parasite & Vectors, v.8:505, 5p, 2015.
1756-3305
10.1186/s13071-015-1117-6
url https://www.arca.fiocruz.br/handle/icict/13094
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/13094/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/13094/2/joao_gesto_etal_IOC_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/13094/3/joao_gesto_etal_IOC_2015.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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