Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/61089 |
Resumo: | Institutional Review Board Statement: The study was conducted in accordance and under approval of the Ethical Committee of Instituto Nacional de Infectologia Evandro Chagas (INI)/Fiocruz, Escola Nacional de Saúde Pública (ENSP)/Fiocruz and Instituto Oswaldo Cruz (IOC)/Fiocruz (protocol code: CAAE:35558920.6.0000.5262, approved on 25 August 2020). |
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Fonseca, Guilherme C. daCavalcante, Liliane T. F.Brustolini, Otávio J.Luz, Paula M.Pires, Debora C.Jalil, Emilia M.Peixoto, Eduardo M.Grinsztejn, BeatrizVeloso, Valdiléa G.Nazer, SandroCosta, Carlos A. M.Villela, Daniel A. M.Goedert, Guilherme T.Santos, Cleber V. B. D.Rodrigues, Nadia C. P.Motta, Fernando do CoutoSiqueira, Marilda MendonçaCoelho, Lara E.Struchiner, Claudio J.Vasconcelos, Ana Tereza R.2023-11-07T23:43:59Z2023-11-07T23:43:59Z2023FONSECA, Guilherme C. da et al. Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants. International Journal of Molecular Sciences, v. 24, p. 1-13, Aug. 2023.1422-0067https://www.arca.fiocruz.br/handle/icict/6108910.3390/ijms2417131461422-0067Institutional Review Board Statement: The study was conducted in accordance and under approval of the Ethical Committee of Instituto Nacional de Infectologia Evandro Chagas (INI)/Fiocruz, Escola Nacional de Saúde Pública (ENSP)/Fiocruz and Instituto Oswaldo Cruz (IOC)/Fiocruz (protocol code: CAAE:35558920.6.0000.5262, approved on 25 August 2020).This work was conducted within the framework of Corona-ômica-RJ (FAPERJ = E26/210.179/2020 e E-26/211.107/2021) and by by Fundação Oswaldo Cruz (Fiocruz Fundraising Office). PML and CJS acknowledge funding from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). LTFC acknowledges funding from Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). ATRV was supported by CNPq (307145/2021-2) and FAPERJ (E-26/201.046/2022). BG acknowledges funding from CNPq (305789/2019-8) and FAPERJ (E-26/202.915/2018; E-26/210.820/2021; _E-26/200.946/2022_). DAMV acknowledges funding from CNPq (312282/2022-2).Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brazil.Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brazil.Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Rio de Janeiro, RJ, Brazil.Fundação Getúlio Vargas. Escola de Matemática Aplicada. Rio de Janeiro, RJ, Brazil.Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social Hesio Cordeiro. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brazil / Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social Hesio Cordeiro. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil.Fundação Getúlio Vargas. Escola de Matemática Aplicada. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brazil.The innate immune system is the first line of defense against pathogens such as the acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The type I-interferon (IFN) response activation during the initial steps of infection is essential to prevent viral replication and tissue damage. SARS-CoV and SARS-CoV-2 can inhibit this activation, and individuals with a dysregulated IFN-I response are more likely to develop severe disease. Several mutations in different variants of SARS-CoV-2 have shown the potential to interfere with the immune system. Here, we evaluated the buffy coat transcriptome of individuals infected with Gamma or Delta variants of SARS-CoV-2. The Delta transcriptome presents more genes enriched in the innate immune response and Gamma in the adaptive immune response. Interactome and enriched promoter analysis showed that Delta could activate the INF-I response more effectively than Gamma. Two mutations in the N protein and one in the nsp6 protein found exclusively in Gamma have already been described as inhibitors of the interferon response pathway. This indicates that the Gamma variant evolved to evade the IFN-I response. Accordingly, in this work, we showed one of the mechanisms that variants of SARS-CoV-2 can use to avoid or interfere with the host Immune system.engMDPIDifferential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDeltaGammaSARS-CoV-2 variantsInnate immune systemType-I interferon responseViral evolutioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.en_US.fl_str_mv |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| title |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| spellingShingle |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants Fonseca, Guilherme C. da Delta Gamma SARS-CoV-2 variants Innate immune system Type-I interferon response Viral evolution |
| title_short |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| title_full |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| title_fullStr |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| title_full_unstemmed |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| title_sort |
Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants |
| author |
Fonseca, Guilherme C. da |
| author_facet |
Fonseca, Guilherme C. da Cavalcante, Liliane T. F. Brustolini, Otávio J. Luz, Paula M. Pires, Debora C. Jalil, Emilia M. Peixoto, Eduardo M. Grinsztejn, Beatriz Veloso, Valdiléa G. Nazer, Sandro Costa, Carlos A. M. Villela, Daniel A. M. Goedert, Guilherme T. Santos, Cleber V. B. D. Rodrigues, Nadia C. P. Motta, Fernando do Couto Siqueira, Marilda Mendonça Coelho, Lara E. Struchiner, Claudio J. Vasconcelos, Ana Tereza R. |
| author_role |
author |
| author2 |
Cavalcante, Liliane T. F. Brustolini, Otávio J. Luz, Paula M. Pires, Debora C. Jalil, Emilia M. Peixoto, Eduardo M. Grinsztejn, Beatriz Veloso, Valdiléa G. Nazer, Sandro Costa, Carlos A. M. Villela, Daniel A. M. Goedert, Guilherme T. Santos, Cleber V. B. D. Rodrigues, Nadia C. P. Motta, Fernando do Couto Siqueira, Marilda Mendonça Coelho, Lara E. Struchiner, Claudio J. Vasconcelos, Ana Tereza R. |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fonseca, Guilherme C. da Cavalcante, Liliane T. F. Brustolini, Otávio J. Luz, Paula M. Pires, Debora C. Jalil, Emilia M. Peixoto, Eduardo M. Grinsztejn, Beatriz Veloso, Valdiléa G. Nazer, Sandro Costa, Carlos A. M. Villela, Daniel A. M. Goedert, Guilherme T. Santos, Cleber V. B. D. Rodrigues, Nadia C. P. Motta, Fernando do Couto Siqueira, Marilda Mendonça Coelho, Lara E. Struchiner, Claudio J. Vasconcelos, Ana Tereza R. |
| dc.subject.en.en_US.fl_str_mv |
Delta Gamma SARS-CoV-2 variants Innate immune system Type-I interferon response Viral evolution |
| topic |
Delta Gamma SARS-CoV-2 variants Innate immune system Type-I interferon response Viral evolution |
| description |
Institutional Review Board Statement: The study was conducted in accordance and under approval of the Ethical Committee of Instituto Nacional de Infectologia Evandro Chagas (INI)/Fiocruz, Escola Nacional de Saúde Pública (ENSP)/Fiocruz and Instituto Oswaldo Cruz (IOC)/Fiocruz (protocol code: CAAE:35558920.6.0000.5262, approved on 25 August 2020). |
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2023 |
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2023-11-07T23:43:59Z |
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2023-11-07T23:43:59Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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FONSECA, Guilherme C. da et al. Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants. International Journal of Molecular Sciences, v. 24, p. 1-13, Aug. 2023. |
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https://www.arca.fiocruz.br/handle/icict/61089 |
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1422-0067 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ijms241713146 |
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1422-0067 |
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FONSECA, Guilherme C. da et al. Differential Type-I Interferon Response in Buffy Coat Transcriptome of Individuals Infected with SARS-CoV-2 Gamma and Delta Variants. International Journal of Molecular Sciences, v. 24, p. 1-13, Aug. 2023. 1422-0067 10.3390/ijms241713146 |
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MDPI |
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MDPI |
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