Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil

Detalhes bibliográficos
Autor(a) principal: Brandão, Marcelo L.L.
Data de Publicação: 2014
Outros Autores: Almeida, Davi O., Bispo, Fernanda C. P., Bricio, Silvia M. L., Marin, Victor A., Miagostovich, Marize P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10823
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.
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spelling Brandão, Marcelo L.L.Almeida, Davi O.Bispo, Fernanda C. P.Bricio, Silvia M. L.Marin, Victor A.Miagostovich, Marize P.2015-06-12T13:57:52Z2015-06-12T13:57:52Z2014BRANDÃO, Marcelo L. L. et al. Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil. Journal of Food Science, v.79, n.5, p.M961-M966, 2014.1750-3841https://www.arca.fiocruz.br/handle/icict/1082310.1111/1750-3841.12459engWiley Online LibraryAssessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.This study aimed to assess the microbiological contamination of lettuces commercialized in Rio de Janeiro, Brazil, in order to investigate detection of norovirus genogroup II (NoV GII), Salmonella spp., total and fecal coliforms, such as Escherichia coli. For NoV detection samples were processed using the adsorption-elution concentration method associated to real-time quantitative polymerase chain reaction (qPCR). A total of 90 samples of lettuce including 30 whole fresh lettuces, 30 minimally processed (MP) lettuces, and 30 raw ready-to-eat (RTE) lettuce salads were randomly collected from different supermarkets (fresh and MP lettuce samples), food services, and self-service restaurants (RTE lettuce salads), all located in Rio de Janeiro, Brazil, from October 2010 to December 2011. NoV GII was not detected and PP7 bacteriophage used as internal control process (ICP) was recovered in 40.0%, 86.7%, and 76.7% of those samples, respectively. Salmonella spp. was not detected although fecal contamination has been observed by fecal coliform concentrations higher than 102 most probable number/g. E. coli was detected in 70.0%, 6.7%, and 30.0% of fresh, MP, and RTE samples, respectively. This study highlights the need to improve hygiene procedures at all stages of vegetable production and to show PP7 bacteriophage as an ICP for recovering RNA viruses’ methods from MP and RTE lettuce samples, encouraging the evaluation of new protocols that facilitate the establishment of methodologies for NoV detection in a greater number of food microbiology laboratories.Bacterial contaminationInternal controlNorovirusRT-PCRNorovirusContaminaçãoBactériasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10823/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmarize_maigostovichetal_IOC_2014.pdfapplication/pdf187809https://www.arca.fiocruz.br/bitstream/icict/10823/2/marize_maigostovichetal_IOC_2014.pdfe90a75262dacc1f70029bab23fe1e7b1MD52TEXTmarize_maigostovichetal_IOC_2014.pdf.txtmarize_maigostovichetal_IOC_2014.pdf.txtExtracted texttext/plain37043https://www.arca.fiocruz.br/bitstream/icict/10823/3/marize_maigostovichetal_IOC_2014.pdf.txt716c5c36382cca6485f72781107bab51MD53icict/108232022-06-24 12:17:41.312oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:41Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
title Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
spellingShingle Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
Brandão, Marcelo L.L.
Bacterial contamination
Internal control
Norovirus
RT-PCR
Norovirus
Contaminação
Bactérias
title_short Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
title_full Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
title_fullStr Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
title_full_unstemmed Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
title_sort Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil
author Brandão, Marcelo L.L.
author_facet Brandão, Marcelo L.L.
Almeida, Davi O.
Bispo, Fernanda C. P.
Bricio, Silvia M. L.
Marin, Victor A.
Miagostovich, Marize P.
author_role author
author2 Almeida, Davi O.
Bispo, Fernanda C. P.
Bricio, Silvia M. L.
Marin, Victor A.
Miagostovich, Marize P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brandão, Marcelo L.L.
Almeida, Davi O.
Bispo, Fernanda C. P.
Bricio, Silvia M. L.
Marin, Victor A.
Miagostovich, Marize P.
dc.subject.en.pt_BR.fl_str_mv Bacterial contamination
Internal control
Norovirus
RT-PCR
topic Bacterial contamination
Internal control
Norovirus
RT-PCR
Norovirus
Contaminação
Bactérias
dc.subject.decs.pt_BR.fl_str_mv Norovirus
Contaminação
Bactérias
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Produtos. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-12T13:57:52Z
dc.date.available.fl_str_mv 2015-06-12T13:57:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRANDÃO, Marcelo L. L. et al. Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil. Journal of Food Science, v.79, n.5, p.M961-M966, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10823
dc.identifier.issn.pt_BR.fl_str_mv 1750-3841
dc.identifier.doi.pt_BR.fl_str_mv 10.1111/1750-3841.12459
identifier_str_mv BRANDÃO, Marcelo L. L. et al. Assessment of Microbiological Contamination of Fresh, Minimally Processed, and Ready-to-Eat Lettuces (Lactuca sativa), Rio de Janeiro State, Brazil. Journal of Food Science, v.79, n.5, p.M961-M966, 2014.
1750-3841
10.1111/1750-3841.12459
url https://www.arca.fiocruz.br/handle/icict/10823
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley Online Library
publisher.none.fl_str_mv Wiley Online Library
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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