Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence

Detalhes bibliográficos
Autor(a) principal: Muñoz-Calderón, Arturo
Data de Publicação: 2021
Outros Autores: Gomess, Natalia Lins Silva, Apodaca, Sofia, Noya, Belkisyolé Alarcón de, Díaz-Bello, Zoraida, Souza, Leticia Rocha Quintino, Costa, Alexandre Dias Tavares, Britto, Constança, Moreira, Otacilio Cruz, Schijman, Alejandro G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46584
Resumo: Laboratorio de Biología Molecular de la Enfermedad de Chagas. Instituto de Ingeniería Genética y Biología Molecular “Dr Héctor Torres” (INGEBI), Buenos Aires, Argentina.
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spelling Muñoz-Calderón, ArturoGomess, Natalia Lins SilvaApodaca, SofiaNoya, Belkisyolé Alarcón deDíaz-Bello, ZoraidaSouza, Leticia Rocha QuintinoCosta, Alexandre Dias TavaresBritto, ConstançaMoreira, Otacilio CruzSchijman, Alejandro G.2021-04-08T19:29:16Z2021-04-08T19:29:16Z2021MUÑOZ-CALDERON, Arturo et al. Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence. The Journal of Molecular Diagnostics, 13p, 2021.1525-1578https://www.arca.fiocruz.br/handle/icict/4658410.1016/j.jmoldx.2021.01.007engElsevierTrypanosoma cruziPadrão únicoQuantificaçãoPopulações naturaisUnidade de Satélite SintéticoUsoTrypanosoma cruziSingle StandardQuantificationNatural PopulationsUsingSynthetic Satellite UnitToward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratorio de Biología Molecular de la Enfermedad de Chagas. Instituto de Ingeniería Genética y Biología Molecular “Dr Héctor Torres” (INGEBI), Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Laboratorio de Biología Molecular de la Enfermedad de Chagas. Instituto de Ingeniería Genética y Biología Molecular “Dr Héctor Torres” (INGEBI), Buenos Aires, Argentina.the Sección de Inmunologia. Instituto de Medicina Tropical “Dr Félix Pifano. Universidad Central de Venezuela, Caracas, Venezuela.the Sección de Inmunologia,z Instituto de Medicina Tropical “Dr Félix Pifano,” Universidad Central de Venezuela, Caracas, Venezuela;Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidad Central de Venezuela, Caracas, Venezuela / Laboratório de Ciências e Tecnologias Aplicadas à Saúde (LaCTAS). Venezuela.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Laboratorio de Biología Molecular de la Enfermedad de Chagas. Instituto de Ingeniería Genética y Biología Molecular “Dr Héctor Torres” (INGEBI), Buenos Aires, Argentina.Accurate diagnostic tools and surrogate markers of parasitologic response to treatment are needed for managing Chagas disease. Quantitative real-time PCR (qPCR) is used for treatment monitoring, but vari ability in copy dosage and sequences of molecular target genes among different Trypanosoma cruzi strains limit the precision of quantitative measures. To improve qPCR quantification accuracy, we designed and evaluated a synthetic DNA molecule containing a satellite DNA (satDNA) repeat unit as standard for quantification of T. cruzi loads in clinical samples, independently of the parasite strain. Probit regression analysis established for Dm28c (TcI) and CL-Brener (TcVI) stocks similar 95% limit of detection values [0.903 (0.745 to 1.497) and 0.667 (CI, 0.113 to 3.927) copy numbers/mL, respectively] when synthetic DNA was the standard for quantification, allowing direct comparison of loads in samples infected with different discrete typing units. This standard curve was evaluated in 205 samples (38 acute oral and 19 chronic Chagas disease patients) from different geographical areas infected with various genotypes, including samples obtained during treatment follow-up; high agreement with parasitic load trends using standard curves based on DNA extracted from spiked blood with counted parasites was obtained. This qPCR-based quantification strategy will be a valuable tool in phase 3 clinical trials, to follow up patients under treatment or at risk of reac tivation, and in experimental models using different parasite strains.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46584/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdfConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdfapplication/pdf2282238https://www.arca.fiocruz.br/bitstream/icict/46584/2/ConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdfe04a0d6a780591e80c3708098fb7a534MD52TEXTConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdf.txtConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
title Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
spellingShingle Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
Muñoz-Calderón, Arturo
Trypanosoma cruzi
Padrão único
Quantificação
Populações naturais
Unidade de Satélite Sintético
Uso
Trypanosoma cruzi
Single Standard
Quantification
Natural Populations
Using
Synthetic Satellite Unit
title_short Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
title_full Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
title_fullStr Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
title_full_unstemmed Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
title_sort Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence
author Muñoz-Calderón, Arturo
author_facet Muñoz-Calderón, Arturo
Gomess, Natalia Lins Silva
Apodaca, Sofia
Noya, Belkisyolé Alarcón de
Díaz-Bello, Zoraida
Souza, Leticia Rocha Quintino
Costa, Alexandre Dias Tavares
Britto, Constança
Moreira, Otacilio Cruz
Schijman, Alejandro G.
author_role author
author2 Gomess, Natalia Lins Silva
Apodaca, Sofia
Noya, Belkisyolé Alarcón de
Díaz-Bello, Zoraida
Souza, Leticia Rocha Quintino
Costa, Alexandre Dias Tavares
Britto, Constança
Moreira, Otacilio Cruz
Schijman, Alejandro G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Muñoz-Calderón, Arturo
Gomess, Natalia Lins Silva
Apodaca, Sofia
Noya, Belkisyolé Alarcón de
Díaz-Bello, Zoraida
Souza, Leticia Rocha Quintino
Costa, Alexandre Dias Tavares
Britto, Constança
Moreira, Otacilio Cruz
Schijman, Alejandro G.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Padrão único
Quantificação
Populações naturais
Unidade de Satélite Sintético
Uso
topic Trypanosoma cruzi
Padrão único
Quantificação
Populações naturais
Unidade de Satélite Sintético
Uso
Trypanosoma cruzi
Single Standard
Quantification
Natural Populations
Using
Synthetic Satellite Unit
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Single Standard
Quantification
Natural Populations
Using
Synthetic Satellite Unit
description Laboratorio de Biología Molecular de la Enfermedad de Chagas. Instituto de Ingeniería Genética y Biología Molecular “Dr Héctor Torres” (INGEBI), Buenos Aires, Argentina.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-04-08T19:29:16Z
dc.date.available.fl_str_mv 2021-04-08T19:29:16Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MUÑOZ-CALDERON, Arturo et al. Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence. The Journal of Molecular Diagnostics, 13p, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46584
dc.identifier.issn.pt_BR.fl_str_mv 1525-1578
dc.identifier.doi.none.fl_str_mv 10.1016/j.jmoldx.2021.01.007
identifier_str_mv MUÑOZ-CALDERON, Arturo et al. Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence. The Journal of Molecular Diagnostics, 13p, 2021.
1525-1578
10.1016/j.jmoldx.2021.01.007
url https://www.arca.fiocruz.br/handle/icict/46584
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/46584/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/46584/2/ConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdf
https://www.arca.fiocruz.br/bitstream/icict/46584/3/ConstancaBrito_OtaciioMoreira_etal_IOC_2021.pdf.txt
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