Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva

Detalhes bibliográficos
Autor(a) principal: Menezes, Maria José
Data de Publicação: 2008
Outros Autores: Costa, Dirceu Joaquim, Clarêncio, Jorge, Miranda, José Carlos, Barral, Aldina Maria Prado, Barral Netto, Manoel, Brodskyn, Claudia Ida, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/2802
Resumo: This work was supported by grants from CNPq, FAPESB and PAPES/FIOCRUZ. M.J.M. was supported by a CAPES fellowship;
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spelling Menezes, Maria JoséCosta, Dirceu JoaquimClarêncio, JorgeMiranda, José CarlosBarral, Aldina Maria PradoBarral Netto, ManoelBrodskyn, Claudia IdaOliveira, Camila Indiani de2011-08-20T22:35:31Z2011-08-20T22:35:31Z2008MENEZES, M. J. et al. Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva. BMC Immunology, v. 9, n. 12, p. 1-8, 20081471-2172https://www.arca.fiocruz.br/handle/icict/280210.1186/1471-2172-9-12This work was supported by grants from CNPq, FAPESB and PAPES/FIOCRUZ. M.J.M. was supported by a CAPES fellowship;Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. BrasilSand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis.Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-alpha, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes.Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitismengImmunomodulation of human monocytes following exposure to Lutzomyia intermedia salivainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmanioseLeishmania braziliensisMonócitosPsychodidaeSalivaCitocinasCitometria deFluxoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALP Immunomodulation of human monocytes following exposure.pdfP Immunomodulation of human monocytes following exposure.pdfapplication/pdf458239https://www.arca.fiocruz.br/bitstream/icict/2802/1/P%20Immunomodulation%20of%20human%20monocytes%20following%20exposure.pdfc5ba1f44bc1d45b39d985226739a8802MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2802/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTP Immunomodulation of human monocytes following exposure.pdf.txtP Immunomodulation of human monocytes following exposure.pdf.txtExtracted texttext/plain30731https://www.arca.fiocruz.br/bitstream/icict/2802/5/P%20Immunomodulation%20of%20human%20monocytes%20following%20exposure.pdf.txt623b71919553f8709647ea64a09dfd5bMD55THUMBNAILP Immunomodulation of human monocytes following exposure.pdf.jpgP Immunomodulation of human monocytes following exposure.pdf.jpgGenerated Thumbnailimage/jpeg2384https://www.arca.fiocruz.br/bitstream/icict/2802/4/P%20Immunomodulation%20of%20human%20monocytes%20following%20exposure.pdf.jpg5cc07f7c61b839983559533d76f008d0MD54icict/28022023-03-15 14:33:14.863oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
title Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
spellingShingle Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
Menezes, Maria José
Leishmaniose
Leishmania braziliensis
Monócitos
Psychodidae
Saliva
Citocinas
Citometria deFluxo
title_short Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
title_full Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
title_fullStr Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
title_full_unstemmed Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
title_sort Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
author Menezes, Maria José
author_facet Menezes, Maria José
Costa, Dirceu Joaquim
Clarêncio, Jorge
Miranda, José Carlos
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
author_role author
author2 Costa, Dirceu Joaquim
Clarêncio, Jorge
Miranda, José Carlos
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes, Maria José
Costa, Dirceu Joaquim
Clarêncio, Jorge
Miranda, José Carlos
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose
Leishmania braziliensis
Monócitos
Psychodidae
Saliva
Citocinas
Citometria deFluxo
topic Leishmaniose
Leishmania braziliensis
Monócitos
Psychodidae
Saliva
Citocinas
Citometria deFluxo
description This work was supported by grants from CNPq, FAPESB and PAPES/FIOCRUZ. M.J.M. was supported by a CAPES fellowship;
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2011-08-20T22:35:31Z
dc.date.available.fl_str_mv 2011-08-20T22:35:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MENEZES, M. J. et al. Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva. BMC Immunology, v. 9, n. 12, p. 1-8, 2008
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/2802
dc.identifier.issn.none.fl_str_mv 1471-2172
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2172-9-12
identifier_str_mv MENEZES, M. J. et al. Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva. BMC Immunology, v. 9, n. 12, p. 1-8, 2008
1471-2172
10.1186/1471-2172-9-12
url https://www.arca.fiocruz.br/handle/icict/2802
dc.language.iso.fl_str_mv eng
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