Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20936 |
Resumo: | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil. |
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Santos, Fred Luciano NevesCeledon, Paola Alejandra FioraniZanchin, Nilson Ivo ToninLeitolis, AmandaCrestani, SandraFoti, LeonardoSouza, Wayner Vieira deGomes, Yara de MirandaKrieger, Marco Aurélio2017-09-06T19:44:57Z2017-09-06T19:44:57Z2017SANTOS, Fred Luciano Neves et al. Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. Journal of Clinical Microbiology, v. 55, n. 10, p. 2934-2945, 2017.1098-660Xhttps://www.arca.fiocruz.br/handle/icict/2093610.1128/JCM.00851-17porAmerican Society For MicrobiologyTrypanosoma cruziChagas DiseaseMutant Chimeric ProteinsAntigensEnfermedad de ChagasProteínas Mutantes QuiméricasAntígenosDoença de ChagasProteínas Mutantes QuiméricasAntígenosPerformance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assaysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil.Diagnosing chronic Chagas disease (CD) requires antibody--antigen detection methods, traditionally based on enzymatic assay techniques whose performance depend on the type and quality of antigen used. Previously, 4 recombinant chimeric proteins from Instituto de Biologia Molecular do Paraná (IBMP-8.1 to -8.4) comprising immuno-dominant regions of diverse Trypanosoma cruzi antigens showed excellent diagnostic performance in enzyme-linked immunosorbent assays. Considering that next-generation platforms offer improved CD diagnostic accuracy with different T. cruzi-specific recombinant antigens, we assessed the performance of these chimeras in liquid microarrays (LMAs). The chimeric proteins were expressed in Escherichia coli and purified by chromatography. Sera from 653 chagasic and 680 healthy individuals were used to assess the performance of these chimeras in detecting specific anti-T. cruzi antibodies. Accuracies ranged from 98.1--99.3%, and diagnostic odds ratio values were 3,548 for IBMP-8.3, 4,826 for IBMP-8.1, 7,882 for IBMP-8.2, and 25,000 for IBMP-8.4. A separate sera bank (851 samples) was employed to assess cross-reactivity with other tropical diseases. Leishmania spp., a pathogen with high genome sequence similar to T. cruzi, showed cross-reactivity rates ranging from 0--2.17%. Inconclusive results were negligible (0--0.71%). Bland--Altman and Deming regression analysis based on 200 randomly selected CD-positive and -negative samples demonstrated interchangeability with respect to CD diagnostic performance in both singleplex and multiplex assays. Our results suggested that these chimeras can potentially replace antigens currently used in commercially available assay kits. Moreover, the use of a multiplex platform, like LMA assays employing 2 or more IBMP antigens, would abrogate the need for 2 different testing techniques when diagnosing CD.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20936/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALSantosperformanc.pdfSantosperformanc.pdfapplication/pdf1621790https://www.arca.fiocruz.br/bitstream/icict/20936/2/Santosperformanc.pdfe95251c68d55c20759b5a522c3f9599aMD52TEXTSantosperformanc.pdf.txtSantosperformanc.pdf.txtExtracted texttext/plain47665https://www.arca.fiocruz.br/bitstream/icict/20936/3/Santosperformanc.pdf.txtc3c9ac98479c127da3956293c57af19cMD53icict/209362022-06-24 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| dc.title.pt_BR.fl_str_mv |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| title |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| spellingShingle |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays Santos, Fred Luciano Neves Trypanosoma cruzi Chagas Disease Mutant Chimeric Proteins Antigens Enfermedad de Chagas Proteínas Mutantes Quiméricas Antígenos Doença de Chagas Proteínas Mutantes Quiméricas Antígenos |
| title_short |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| title_full |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| title_fullStr |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| title_full_unstemmed |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| title_sort |
Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays |
| author |
Santos, Fred Luciano Neves |
| author_facet |
Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Zanchin, Nilson Ivo Tonin Leitolis, Amanda Crestani, Sandra Foti, Leonardo Souza, Wayner Vieira de Gomes, Yara de Miranda Krieger, Marco Aurélio |
| author_role |
author |
| author2 |
Celedon, Paola Alejandra Fiorani Zanchin, Nilson Ivo Tonin Leitolis, Amanda Crestani, Sandra Foti, Leonardo Souza, Wayner Vieira de Gomes, Yara de Miranda Krieger, Marco Aurélio |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Zanchin, Nilson Ivo Tonin Leitolis, Amanda Crestani, Sandra Foti, Leonardo Souza, Wayner Vieira de Gomes, Yara de Miranda Krieger, Marco Aurélio |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi |
| topic |
Trypanosoma cruzi Chagas Disease Mutant Chimeric Proteins Antigens Enfermedad de Chagas Proteínas Mutantes Quiméricas Antígenos Doença de Chagas Proteínas Mutantes Quiméricas Antígenos |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas Disease Mutant Chimeric Proteins Antigens |
| dc.subject.es.pt_BR.fl_str_mv |
Enfermedad de Chagas Proteínas Mutantes Quiméricas Antígenos |
| dc.subject.decs.pt_BR.fl_str_mv |
Doença de Chagas Proteínas Mutantes Quiméricas Antígenos |
| description |
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil. |
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2017 |
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2017-09-06T19:44:57Z |
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2017-09-06T19:44:57Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SANTOS, Fred Luciano Neves et al. Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. Journal of Clinical Microbiology, v. 55, n. 10, p. 2934-2945, 2017. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/20936 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1098-660X |
| dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.00851-17 |
| identifier_str_mv |
SANTOS, Fred Luciano Neves et al. Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. Journal of Clinical Microbiology, v. 55, n. 10, p. 2934-2945, 2017. 1098-660X 10.1128/JCM.00851-17 |
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American Society For Microbiology |
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American Society For Microbiology |
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