Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway

Lonien, Sandra C. H.; Malvezi, Aparecida Donizette; Suzukawa, Helena T.; Yamauchi, Lucy Megumi; Yamada-Ogatta, Sueli Fumie; Rizzo, Luiz V.; Bordignon, Juliano; Pinge-Filho, Phileno

Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway

Detalhes bibliográficos
Autor(a) principal:	Lonien, Sandra C. H.
Data de Publicação:	2017
Outros Autores:	Malvezi, Aparecida Donizette, Suzukawa, Helena T., Yamauchi, Lucy Megumi, Yamada-Ogatta, Sueli Fumie, Rizzo, Luiz V., Bordignon, Juliano, Pinge-Filho, Phileno
Tipo de documento:	Artigo
Idioma:	por
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/24927
Resumo:	Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil.

Metadados do item

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spelling	Lonien, Sandra C. H.Malvezi, Aparecida DonizetteSuzukawa, Helena T.Yamauchi, Lucy MegumiYamada-Ogatta, Sueli FumieRizzo, Luiz V.Bordignon, JulianoPinge-Filho, Phileno2018-02-23T19:51:52Z2018-02-23T19:51:52Z2017LONIEN, Sandra C. H. et al. Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway. Frontiers in Microbiology, v. 8, n. 2020, p. 1-11, 2017.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/2492710.3389/fmicb.2017.0202010.3389/fmicb.2017.02020porFrontiers MediaTrypanosoma cruziDendritic CellsAspirinCelecoxibCélulas DendríticasAspirinCelecoxibCélulas DendríticasAspirinaCelecoxibResponse to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathwayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos, Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos, Londrina, PR, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil.Chagas disease (Cd) or American human trypanosomiasis is caused by Trypanosoma cruzi and affects ~7 million people, mostly in Latin America. The infective trypomastigote forms of the parasite can invade several human blood cell populations, including monocytes and dendritic cells (DC). Although these cells display a wide functional diversity, their interactions with T. cruzi via cyclooxygenase (COX) and cyclic adenosine monophosphate (cAMP) dependent pathways have not been analyzed. To exploiting this mechanism, DC-enriched peripheral human blood mononuclear cell populations (DC-PBMC) were used as our model. Our results showed that the treatment of these cell populations with celecoxib (CEL), a cyclooxygenase-2 selective inhibitor or SQ 22,536, an adenilate cyclase inhibitor, significantly caused marked inhibition of T. cruzi infection. In contrast, aspirin (ASA, a non-selective COX-1 and COX-2 inhibitor) treatment did not inhibit the infection of the cells by the parasite and was independent of nitric oxide (NO) production. The expression of co-stimulatory molecules CD80 and CD86 were similar on cells treated or not with both COX-inhibitors. The infection stimulated the release of TNF-α, IL-1β, IL-6, IL-8, and IL-10 production by infected cells. Treatment with ASA or CEL did not affect TNF-α, IL-6, IL-8, IL-10, and NO production by infected cells, but increased IL-1β production by them. Our results suggest a key role of COX-2 and cAMP pathways in T. cruzi invasion process of human blood cells and these pathways may represent targets of new therapeutic options for Cd.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/24927/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALBordignonResp.pdfBordignonResp.pdfapplication/pdf2492354https://www.arca.fiocruz.br/bitstream/icict/24927/2/BordignonResp.pdfcd289add9403f431952d48f2a89cd7feMD52TEXTBordignonResp.pdf.txtBordignonResp.pdf.txtExtracted texttext/plain45192https://www.arca.fiocruz.br/bitstream/icict/24927/3/BordignonResp.pdf.txtef5e2b2a98e0727aa90dfd377206dc65MD53icict/249272019-12-15 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dc.title.pt_BR.fl_str_mv	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
title	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
spellingShingle	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway Lonien, Sandra C. H. Trypanosoma cruzi Dendritic Cells Aspirin Celecoxib Células Dendríticas Aspirin Celecoxib Células Dendríticas Aspirina Celecoxib
title_short	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
title_full	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
title_fullStr	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
title_full_unstemmed	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
title_sort	Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway
author	Lonien, Sandra C. H.
author_facet	Lonien, Sandra C. H. Malvezi, Aparecida Donizette Suzukawa, Helena T. Yamauchi, Lucy Megumi Yamada-Ogatta, Sueli Fumie Rizzo, Luiz V. Bordignon, Juliano Pinge-Filho, Phileno
author_role	author
author2	Malvezi, Aparecida Donizette Suzukawa, Helena T. Yamauchi, Lucy Megumi Yamada-Ogatta, Sueli Fumie Rizzo, Luiz V. Bordignon, Juliano Pinge-Filho, Phileno
author2_role	author author author author author author author
dc.contributor.author.fl_str_mv	Lonien, Sandra C. H. Malvezi, Aparecida Donizette Suzukawa, Helena T. Yamauchi, Lucy Megumi Yamada-Ogatta, Sueli Fumie Rizzo, Luiz V. Bordignon, Juliano Pinge-Filho, Phileno
dc.subject.other.pt_BR.fl_str_mv	Trypanosoma cruzi
topic	Trypanosoma cruzi Dendritic Cells Aspirin Celecoxib Células Dendríticas Aspirin Celecoxib Células Dendríticas Aspirina Celecoxib
dc.subject.en.pt_BR.fl_str_mv	Dendritic Cells Aspirin Celecoxib
dc.subject.es.pt_BR.fl_str_mv	Células Dendríticas Aspirin Celecoxib
dc.subject.decs.pt_BR.fl_str_mv	Células Dendríticas Aspirina Celecoxib
description	Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil.
publishDate	2017
dc.date.issued.fl_str_mv	2017
dc.date.accessioned.fl_str_mv	2018-02-23T19:51:52Z
dc.date.available.fl_str_mv	2018-02-23T19:51:52Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	LONIEN, Sandra C. H. et al. Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway. Frontiers in Microbiology, v. 8, n. 2020, p. 1-11, 2017.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/24927
dc.identifier.issn.pt_BR.fl_str_mv	1664-302X
dc.identifier.doi.pt_BR.fl_str_mv	10.3389/fmicb.2017.02020
dc.identifier.eissn.none.fl_str_mv	10.3389/fmicb.2017.02020
identifier_str_mv	LONIEN, Sandra C. H. et al. Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway. Frontiers in Microbiology, v. 8, n. 2020, p. 1-11, 2017. 1664-302X 10.3389/fmicb.2017.02020
url	https://www.arca.fiocruz.br/handle/icict/24927
dc.language.iso.fl_str_mv	por
language	por
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Frontiers Media
publisher.none.fl_str_mv	Frontiers Media
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway

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