Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25176 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brasil |
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Cota, Betania BarrosTunes, Luiza GuimarãesMaia, Daniela Nabak BuenoRamos, Jonas PereiraOliveira, Djalma Menezes deKohlhoff, MarkusAlves, Tânia Maria de AlmeidaFagundes, Elaine Maria SouzaCampos, Fernanda FragaZani, Carlos Leomar2018-03-07T17:38:29Z2018-03-07T17:38:29Z2018COTA, Betania Barros et al. Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood). Mem. Inst. Oswaldo Cruz, v. 113, n. 2, p. 102-110, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/2517610.1590/0074-02760170217engInstituto Oswaldo CruzLeishmania braziliensisdoenças neglicenciadasfungal metabolitesnatural productsantileishmanialLeishmania braziliensisneglected diseaseLeishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Genômica Funcional e Proteômica de Leishmania spp. e Trypanosoma cruzi. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, BrasilUniversidade Estadual do Sudoeste da Bahia. Departamento de Química. Jequié, BA, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, BrasilUniversidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Ciências Biológicas e da Saúde. Diamantina, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, BrasilBACKGROUND: In a screen of extracts from plants and fungi to detect antileishmanial activity, we found that the ethyl acetate extract of the fungus Nectria pseudotrichia, isolated from the tree Caesalpinia echinata (Brazilwood), is a promising source of bioactive compounds. OBJECTIVES: The aims of this study were to isolate and determine the chemical structures of the compounds responsible for the antileishmanial activity of the organic extract from N. pseudotrichia. METHODS: Compounds were isolated by chromatographic fractionation using semi-preparative high-performance liquid chromatography, and their chemical structures were determined by analytical and spectral data and by comparison with published data. The antileishmanial activity of the isolated compounds was evaluated in intracellular amastigote forms of Leishmania (Viannia) braziliensis expressing firefly luciferase as reporter gene, and cytotoxicity was determined in Vero and THP-1 mammalian cell lines by MTT assay. FINDINGS: Fractionation of the extract yielded seven compounds: 10-acetyl trichoderonic acid A (1), 6′-acetoxy-piliformic acid (2), 5′,6′-dehydropiliformic acid (3), piliformic acid (4), hydroheptelidic acid (5), xylaric acid D (6), and cytochalasin D (7). Compounds 1, 2 and 3 are reported here for the first time. Compounds 1, 2, and 5 were more active, with IC50 values of 21.4, 28.3, and 24.8 µM, respectively, and showed low toxicity to Vero and THP-1 cells. MAIN CONCLUSIONS: N. pseudotrichia produces secondary metabolites that are more toxic to intracellular amastigote forms of L. (V.) braziliensis than to mammalian cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/25176/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALLeishmanicidal compounds of Nectria pseudotrichia.pdfLeishmanicidal compounds of Nectria pseudotrichia.pdfapplication/pdf404788https://www.arca.fiocruz.br/bitstream/icict/25176/2/Leishmanicidal%20compounds%20of%20Nectria%20pseudotrichia.pdfd3f619503129576e6ba497bfcd75b025MD52TEXTLeishmanicidal compounds of Nectria pseudotrichia.pdf.txtLeishmanicidal compounds of Nectria pseudotrichia.pdf.txtExtracted texttext/plain40576https://www.arca.fiocruz.br/bitstream/icict/25176/3/Leishmanicidal%20compounds%20of%20Nectria%20pseudotrichia.pdf.txt017bb14353c5e46542a180fd2ac8a21fMD53icict/251762019-06-19 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| dc.title.pt_BR.fl_str_mv |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| title |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| spellingShingle |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) Cota, Betania Barros Leishmania braziliensis doenças neglicenciadas fungal metabolites natural products antileishmanial Leishmania braziliensis neglected disease |
| title_short |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| title_full |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| title_fullStr |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| title_full_unstemmed |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| title_sort |
Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood) |
| author |
Cota, Betania Barros |
| author_facet |
Cota, Betania Barros Tunes, Luiza Guimarães Maia, Daniela Nabak Bueno Ramos, Jonas Pereira Oliveira, Djalma Menezes de Kohlhoff, Markus Alves, Tânia Maria de Almeida Fagundes, Elaine Maria Souza Campos, Fernanda Fraga Zani, Carlos Leomar |
| author_role |
author |
| author2 |
Tunes, Luiza Guimarães Maia, Daniela Nabak Bueno Ramos, Jonas Pereira Oliveira, Djalma Menezes de Kohlhoff, Markus Alves, Tânia Maria de Almeida Fagundes, Elaine Maria Souza Campos, Fernanda Fraga Zani, Carlos Leomar |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cota, Betania Barros Tunes, Luiza Guimarães Maia, Daniela Nabak Bueno Ramos, Jonas Pereira Oliveira, Djalma Menezes de Kohlhoff, Markus Alves, Tânia Maria de Almeida Fagundes, Elaine Maria Souza Campos, Fernanda Fraga Zani, Carlos Leomar |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania braziliensis doenças neglicenciadas |
| topic |
Leishmania braziliensis doenças neglicenciadas fungal metabolites natural products antileishmanial Leishmania braziliensis neglected disease |
| dc.subject.en.pt_BR.fl_str_mv |
fungal metabolites natural products antileishmanial Leishmania braziliensis neglected disease |
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Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brasil |
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2018 |
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2018-03-07T17:38:29Z |
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2018-03-07T17:38:29Z |
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2018 |
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info:eu-repo/semantics/article |
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COTA, Betania Barros et al. Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood). Mem. Inst. Oswaldo Cruz, v. 113, n. 2, p. 102-110, 2018. |
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https://www.arca.fiocruz.br/handle/icict/25176 |
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0074-0276 |
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10.1590/0074-02760170217 |
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COTA, Betania Barros et al. Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood). Mem. Inst. Oswaldo Cruz, v. 113, n. 2, p. 102-110, 2018. 0074-0276 10.1590/0074-02760170217 |
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