Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis

Detalhes bibliográficos
Autor(a) principal: Romão, Raquel Peralva Ribeiro
Data de Publicação: 2016
Outros Autores: Saavedra, Andrea Franco, Cruz, Alda Maria da, Pinto, Eduardo Fonseca, Moreira, Otacilio C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17893
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.
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spelling Romão, Raquel Peralva RibeiroSaavedra, Andrea FrancoCruz, Alda Maria daPinto, Eduardo FonsecaMoreira, Otacilio C.2017-02-23T15:48:47Z2017-02-23T15:48:47Z2016ROMÃO, Raquel Peralva Ribeiro; et al.Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis. Parasites & Vectors, v.9:361, 12p, 2016.1756-3305https://www.arca.fiocruz.br/handle/icict/1789310.1186/s13071-016-1647-6engBioMed CentralLeishmaniose cutâneaReação em Cadeia da PolimeraseCarga parasitáriaResposta imuneHamsterCutaneous leishmaniasisreal-time PCRgolden hamsterimmune responseparasite loadLeishmania (Viannia) braziliensisDevelopment of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Cutaneous leishmaniasis (CL) is a neglected disease with a broad spectrum of clinical manifestations, ranging from small cutaneous nodules to severe mucosal tissue destruction. Leishmania (Viannia) braziliensis is the main species attributed to CL in the Americas. However, studies of experimental infection are limited in the murine model due to the self-resolutive pattern of the disease. Previously, our group demonstrated that the hamster model reproduces many of the clinical and histopathological features observed in humans. Herein, we standardized a RT-qPCR gene expression assay to evaluate a panel of immunological markers and a qPCR assay in order to quantify with high sensitivity and reproducibility the parasite load in skin lesions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17893/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALoctacilio_moreira_etal_IOC_2016.pdfoctacilio_moreira_etal_IOC_2016.pdfapplication/pdf3840446https://www.arca.fiocruz.br/bitstream/icict/17893/2/octacilio_moreira_etal_IOC_2016.pdf0d27fcfdafc79080420cd9236cca192eMD52TEXToctacilio_moreira_etal_IOC_2016.pdf.txtoctacilio_moreira_etal_IOC_2016.pdf.txtExtracted texttext/plain53222https://www.arca.fiocruz.br/bitstream/icict/17893/3/octacilio_moreira_etal_IOC_2016.pdf.txt27f27202f107008de3d8b33cb3b25214MD53icict/178932022-06-24 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dc.title.pt_BR.fl_str_mv Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
title Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
spellingShingle Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
Romão, Raquel Peralva Ribeiro
Leishmaniose cutânea
Reação em Cadeia da Polimerase
Carga parasitária
Resposta imune
Hamster
Cutaneous leishmaniasis
real-time PCR
golden hamster
immune response
parasite load
Leishmania (Viannia) braziliensis
title_short Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
title_full Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
title_fullStr Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
title_full_unstemmed Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
title_sort Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
author Romão, Raquel Peralva Ribeiro
author_facet Romão, Raquel Peralva Ribeiro
Saavedra, Andrea Franco
Cruz, Alda Maria da
Pinto, Eduardo Fonseca
Moreira, Otacilio C.
author_role author
author2 Saavedra, Andrea Franco
Cruz, Alda Maria da
Pinto, Eduardo Fonseca
Moreira, Otacilio C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Romão, Raquel Peralva Ribeiro
Saavedra, Andrea Franco
Cruz, Alda Maria da
Pinto, Eduardo Fonseca
Moreira, Otacilio C.
dc.subject.other.pt_BR.fl_str_mv Leishmaniose cutânea
Reação em Cadeia da Polimerase
Carga parasitária
Resposta imune
Hamster
topic Leishmaniose cutânea
Reação em Cadeia da Polimerase
Carga parasitária
Resposta imune
Hamster
Cutaneous leishmaniasis
real-time PCR
golden hamster
immune response
parasite load
Leishmania (Viannia) braziliensis
dc.subject.en.pt_BR.fl_str_mv Cutaneous leishmaniasis
real-time PCR
golden hamster
immune response
parasite load
Leishmania (Viannia) braziliensis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-23T15:48:47Z
dc.date.available.fl_str_mv 2017-02-23T15:48:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROMÃO, Raquel Peralva Ribeiro; et al.Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis. Parasites & Vectors, v.9:361, 12p, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17893
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-016-1647-6
identifier_str_mv ROMÃO, Raquel Peralva Ribeiro; et al.Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis. Parasites & Vectors, v.9:361, 12p, 2016.
1756-3305
10.1186/s13071-016-1647-6
url https://www.arca.fiocruz.br/handle/icict/17893
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/17893/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/17893/2/octacilio_moreira_etal_IOC_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/17893/3/octacilio_moreira_etal_IOC_2016.pdf.txt
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