HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals

Detalhes bibliográficos
Autor(a) principal: Cunha, Joana Paixão Monteiro
Data de Publicação: 2017
Outros Autores: Souza, Juliana Sacramento Mota de, Diaz, Ricardo Sobhie, Rego, Filipe Ferreira de Almeida, Andrade, E, Silva, Márcio de Oliveira, Marta, Giovanetti, Oliveira, T. de, Oliveira, A, Alves, Carlos Roberto Brites, Alcantara, Luiz Carlos Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26766
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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spelling Cunha, Joana Paixão MonteiroSouza, Juliana Sacramento Mota deDiaz, Ricardo SobhieRego, Filipe Ferreira de AlmeidaAndrade, ESilva, Márcio de OliveiraMarta, GiovanettiOliveira, T. deOliveira, AAlves, Carlos Roberto BritesAlcantara, Luiz Carlos Júnior2018-06-07T14:08:10Z2018-06-07T14:08:10Z2017CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017.2454-499Xhttps://www.arca.fiocruz.br/handle/icict/26766Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Federal University of Bahia. Core Bioinformatics Department Bioformatics. Salvador, BA, Brazil / Bahia School of Medicine and Public Health. Bahia Foundation for the Development of Sciences. Salvador, BA, BrazilFederal University of Bahia. Core Bioinformatics Department Bioformatics. Salvador, BA, BrazilFederal University of São Paulo. Department of Medicine. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilUniversity of KwaZulu-Natal. Africa Centre for Health and Population Studies. Durban, South AfricaState Center Specialized in Diagnosis. Assistance and Research. Salvador, BA, BrazilFederal University of Bahia. Professor Edgard Santos University Hospital Complex. Laboratory of Infectology Research. Salvador, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil499XThe distribution of different human immunodeficiency virus type 1 (HIV-1) genotypes and the prevalence of transmitted drug resistance (TDR) mutations vary greatly across different Brazilian regions. This study aimed to describe the HIV-1 molecular diversity and TDR prevalence among treatment-naïve HIV-1 infected individuals in an urban area of Northeastern Brazil. DNA samples from 97 infected individuals were obtained and pol sequences were generated by Polimerase Chain Reaction (PCR) and direct sequencing. Bioinformatics tools were used to identify the presence of associated mutations with drug resistance, to reconstruct the phylogeny and to detect recombination. The median age and estimated time of HIV-1 diagnosis were 34 years and 12 months, respectively and 61% of the patients were male. The main exposure category was sexual (87.5%) and the median TCD4+ lymphocyte counts and viral load were 438 cells/mm3 and 4.4 log10, respectively. The overall TDR prevalence was 5.1% while within each drug class (Non-nucleoside reverse-transcriptase inhibitors - NNRTIs, Nucleoside analog reverse-transcriptase inhibitors - NRTIs and Protease inhibitors - PIs) this rate was 2.1% individually. These TDR mutations confer intermediate to high-level resistance to different drugs that are used as first-line treatment options, which reinforce the importance of genotype testing prior to treatment initia-tion. Phylogenetic analysis showed that 73.2% of the samples were subtype B, 14.4% were subtype F, 4.1% were subtype C and 8.3% were BF recombinants. Our data indicate that the genotypic profile of HIV-1 strains circulating in Bahia is diversified and show an increase of subtype C prevalence in Northeast BrazilengAnnex PublishersGenotipagemMutaçãoAntirretroviralSubtiposHIVResistênciaIntrodução à PrevalênciaGenotypingMutationAntiretroviralSubtypesHIVResistancePrevalence IntroductionHIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26766/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCunha JPM HIV-1-Molecular-Characterization-and-Transmitted-Drug-Resistance-Prevalence-among-Treatment-Naive-Individuals.pdfCunha JPM 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dc.title.pt_BR.fl_str_mv HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
title HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
spellingShingle HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
Cunha, Joana Paixão Monteiro
Genotipagem
Mutação
Antirretroviral
Subtipos
HIV
Resistência
Introdução à Prevalência
Genotyping
Mutation
Antiretroviral
Subtypes
HIV
Resistance
Prevalence Introduction
title_short HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
title_full HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
title_fullStr HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
title_full_unstemmed HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
title_sort HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
author Cunha, Joana Paixão Monteiro
author_facet Cunha, Joana Paixão Monteiro
Souza, Juliana Sacramento Mota de
Diaz, Ricardo Sobhie
Rego, Filipe Ferreira de Almeida
Andrade, E
Silva, Márcio de Oliveira
Marta, Giovanetti
Oliveira, T. de
Oliveira, A
Alves, Carlos Roberto Brites
Alcantara, Luiz Carlos Júnior
author_role author
author2 Souza, Juliana Sacramento Mota de
Diaz, Ricardo Sobhie
Rego, Filipe Ferreira de Almeida
Andrade, E
Silva, Márcio de Oliveira
Marta, Giovanetti
Oliveira, T. de
Oliveira, A
Alves, Carlos Roberto Brites
Alcantara, Luiz Carlos Júnior
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Joana Paixão Monteiro
Souza, Juliana Sacramento Mota de
Diaz, Ricardo Sobhie
Rego, Filipe Ferreira de Almeida
Andrade, E
Silva, Márcio de Oliveira
Marta, Giovanetti
Oliveira, T. de
Oliveira, A
Alves, Carlos Roberto Brites
Alcantara, Luiz Carlos Júnior
dc.subject.other.pt_BR.fl_str_mv Genotipagem
Mutação
Antirretroviral
Subtipos
HIV
Resistência
Introdução à Prevalência
topic Genotipagem
Mutação
Antirretroviral
Subtipos
HIV
Resistência
Introdução à Prevalência
Genotyping
Mutation
Antiretroviral
Subtypes
HIV
Resistance
Prevalence Introduction
dc.subject.en.pt_BR.fl_str_mv Genotyping
Mutation
Antiretroviral
Subtypes
HIV
Resistance
Prevalence Introduction
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-06-07T14:08:10Z
dc.date.available.fl_str_mv 2018-06-07T14:08:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26766
dc.identifier.issn.pt_BR.fl_str_mv 2454-499X
identifier_str_mv CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017.
2454-499X
url https://www.arca.fiocruz.br/handle/icict/26766
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Annex Publishers
publisher.none.fl_str_mv Annex Publishers
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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