Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, Rafaela Moledo de
Data de Publicação: 2008
Outros Autores: Almeida, Antonio Eugenio Castro Cardoso de, Hofer, Ernesto, Silva, Nicéa Magaly Matias da, Marin, Victor Augustus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27577
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde.. Rio de Janeiro, RJ. Brasil.
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spelling Vasconcelos, Rafaela Moledo deAlmeida, Antonio Eugenio Castro Cardoso deHofer, ErnestoSilva, Nicéa Magaly Matias daMarin, Victor Augustus2018-07-17T17:24:30Z2018-07-17T17:24:30Z2008VASCONCELLOS, Rafaela Moledo de; et al. Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens. Mem Inst Oswaldo Cruz, v.103, n.8, p.836-838, Dec. 2008.0074-0276https://www.arca.fiocruz.br/handle/icict/275771678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzListeria monocytogenesReação em Cadeia da Polimerase MultiplexLíquido CefalorraquidianoListerioseListeria monocytogeneslisteriosisMultiplex PCRcerebrospinal fluidMultiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde.. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.Clínica Perinatal de Laranjeiras. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde.. Rio de Janeiro, RJ. Brasil.The genus Listeria is composed of six species of which Listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. The results indicated the presence of L. monocytogenes in the clinical sample studied. Moreover, the isolate was identified as the 4b serovar that was characterized by the presence of a unique 691 bp band after analysis using the Multiplex-PCR technique. The results of repeated Multiplex-PCR and sequencing have indicated that the L. monocytogenes isolate was an atypical 4b serovar, which is the first time this finding has been reported.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27577/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALantonio_almeida_etal_IOC_2008.pdfantonio_almeida_etal_IOC_2008.pdfapplication/pdf283825https://www.arca.fiocruz.br/bitstream/icict/27577/2/antonio_almeida_etal_IOC_2008.pdfa7b395d9b8d9f004266d814d24939146MD52TEXTantonio_almeida_etal_IOC_2008.pdf.txtantonio_almeida_etal_IOC_2008.pdf.txtExtracted texttext/plain15975https://www.arca.fiocruz.br/bitstream/icict/27577/3/antonio_almeida_etal_IOC_2008.pdf.txt1828d1edbb3b777326ff7196f1cd623aMD53icict/275772018-08-15 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dc.title.pt_BR.fl_str_mv Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
title Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
spellingShingle Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
Vasconcelos, Rafaela Moledo de
Listeria monocytogenes
Reação em Cadeia da Polimerase Multiplex
Líquido Cefalorraquidiano
Listeriose
Listeria monocytogenes
listeriosis
Multiplex PCR
cerebrospinal fluid
title_short Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
title_full Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
title_fullStr Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
title_full_unstemmed Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
title_sort Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
author Vasconcelos, Rafaela Moledo de
author_facet Vasconcelos, Rafaela Moledo de
Almeida, Antonio Eugenio Castro Cardoso de
Hofer, Ernesto
Silva, Nicéa Magaly Matias da
Marin, Victor Augustus
author_role author
author2 Almeida, Antonio Eugenio Castro Cardoso de
Hofer, Ernesto
Silva, Nicéa Magaly Matias da
Marin, Victor Augustus
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos, Rafaela Moledo de
Almeida, Antonio Eugenio Castro Cardoso de
Hofer, Ernesto
Silva, Nicéa Magaly Matias da
Marin, Victor Augustus
dc.subject.other.pt_BR.fl_str_mv Listeria monocytogenes
Reação em Cadeia da Polimerase Multiplex
Líquido Cefalorraquidiano
Listeriose
topic Listeria monocytogenes
Reação em Cadeia da Polimerase Multiplex
Líquido Cefalorraquidiano
Listeriose
Listeria monocytogenes
listeriosis
Multiplex PCR
cerebrospinal fluid
dc.subject.en.pt_BR.fl_str_mv Listeria monocytogenes
listeriosis
Multiplex PCR
cerebrospinal fluid
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde.. Rio de Janeiro, RJ. Brasil.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2018-07-17T17:24:30Z
dc.date.available.fl_str_mv 2018-07-17T17:24:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VASCONCELLOS, Rafaela Moledo de; et al. Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens. Mem Inst Oswaldo Cruz, v.103, n.8, p.836-838, Dec. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27577
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv VASCONCELLOS, Rafaela Moledo de; et al. Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens. Mem Inst Oswaldo Cruz, v.103, n.8, p.836-838, Dec. 2008.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/27577
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/27577/2/antonio_almeida_etal_IOC_2008.pdf
https://www.arca.fiocruz.br/bitstream/icict/27577/3/antonio_almeida_etal_IOC_2008.pdf.txt
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