Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37912 |
Resumo: | Universidade Federal de Viçosa. Instituto de Biotecnologia Aplicada à Agricultura e Ciência Animal. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil. |
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Xavier, Luciana PereiraOliveira, Maria Goreti AlmeidaGuedes, Raul Narciso CarvalhoSantos, Agenor ValadaresDe Simone, Salvatore Giovanni2019-12-12T16:14:57Z2019-12-12T16:14:57Z2005XAVIER, Luciana Pereira et al. Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae). Eur. J. Entomol., v. 102, p. 147-153, 2005.1802-8829https://www.arca.fiocruz.br/handle/icict/3791210.14411/eje.2005.023engEuropean Journal of EntomologyTripsinaProteasesLepidópterosTrypsinProteasesVelvetbean caterpillarLepidopteraNoctuidaeAnticarsiaTrypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Viçosa. Instituto de Biotecnologia Aplicada à Agricultura e Ciência Animal. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil.Universidade Federal de Viçosa. Instituto de Biotecnologia Aplicada à Agricultura e Ciência Animal. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil / Universidade Federal de Viçosa. Departamento de Biologia Animal. Viçosa, MG, Brasil.Universidade Federal de Viçosa. Departamento de Biologia Animal. Viçosa, MG, Brasil.Universidade Federal de Viçosa. Departamento de Microbiologia. Instituto de Biotecnologia Aplicada à Agricultura e Ciência Animal. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Membrane-bound proteases from preparations of the midgut of 5th instar velvetbean caterpillars, Anticarsia gemmatalis (Hübner) were obtained by resuspension of the pellet obtained after 100,000 g centrifugation. As expected of trypsin-like proteases, they hydrolyzed casein and the synthetic substrates N--benzoyl-L-Arg-p-nitroanilidine (L-BApNA) and N--p-tosyl-L-Arg methyl ester (L-TAME). Higher activities were observed at 50°C, and at pH 8.5 and 8.0 for both synthetic substrates L-BApNA and L-TAME. The membrane-bound proteases were inhibited by EDTA, phenylmethan sulphonyl fluoride (PMSF), tosyl-L-lysine chloromethyl ketone (TLCK), benzamidine and aprotinin. TLCK and benzamidine were particularly active inhibitors. The KM-values obtained were 0.23 mM for L-BApNA and 92.5 μM for L-TAME. These results provide evidence for the presence of membranebound trypsin-like proteases in the midgut of the velvetbean caterpillar, a key soybean pest in warm climates. The interaction between A. gemmatalis digestive proteases and soybean protease inhibitors has potentially important consequences for soybean breeding programs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37912/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSalvatoreGDe_Simone_etal_IOC_2005.pdfSalvatoreGDe_Simone_etal_IOC_2005.pdfapplication/pdf146728https://www.arca.fiocruz.br/bitstream/icict/37912/2/SalvatoreGDe_Simone_etal_IOC_2005.pdf5105d84e487cc5a30b34ee0fe0f6ac5aMD52TEXTSalvatoreGDe_Simone_etal_IOC_2005.pdf.txtSalvatoreGDe_Simone_etal_IOC_2005.pdf.txtExtracted texttext/plain34683https://www.arca.fiocruz.br/bitstream/icict/37912/3/SalvatoreGDe_Simone_etal_IOC_2005.pdf.txtc6f4d0cfc8a37f0cfed8fcac9dff99f9MD53icict/379122019-12-13 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| dc.title.pt_BR.fl_str_mv |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| title |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| spellingShingle |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) Xavier, Luciana Pereira Tripsina Proteases Lepidópteros Trypsin Proteases Velvetbean caterpillar Lepidoptera Noctuidae Anticarsia |
| title_short |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| title_full |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| title_fullStr |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| title_full_unstemmed |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| title_sort |
Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
| author |
Xavier, Luciana Pereira |
| author_facet |
Xavier, Luciana Pereira Oliveira, Maria Goreti Almeida Guedes, Raul Narciso Carvalho Santos, Agenor Valadares De Simone, Salvatore Giovanni |
| author_role |
author |
| author2 |
Oliveira, Maria Goreti Almeida Guedes, Raul Narciso Carvalho Santos, Agenor Valadares De Simone, Salvatore Giovanni |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Xavier, Luciana Pereira Oliveira, Maria Goreti Almeida Guedes, Raul Narciso Carvalho Santos, Agenor Valadares De Simone, Salvatore Giovanni |
| dc.subject.other.pt_BR.fl_str_mv |
Tripsina Proteases Lepidópteros |
| topic |
Tripsina Proteases Lepidópteros Trypsin Proteases Velvetbean caterpillar Lepidoptera Noctuidae Anticarsia |
| dc.subject.en.pt_BR.fl_str_mv |
Trypsin Proteases Velvetbean caterpillar Lepidoptera Noctuidae Anticarsia |
| description |
Universidade Federal de Viçosa. Instituto de Biotecnologia Aplicada à Agricultura e Ciência Animal. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil. |
| publishDate |
2005 |
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2005 |
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2019-12-12T16:14:57Z |
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2019-12-12T16:14:57Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
XAVIER, Luciana Pereira et al. Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae). Eur. J. Entomol., v. 102, p. 147-153, 2005. |
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https://www.arca.fiocruz.br/handle/icict/37912 |
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1802-8829 |
| dc.identifier.doi.none.fl_str_mv |
10.14411/eje.2005.023 |
| identifier_str_mv |
XAVIER, Luciana Pereira et al. Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae). Eur. J. Entomol., v. 102, p. 147-153, 2005. 1802-8829 10.14411/eje.2005.023 |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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European Journal of Entomology |
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European Journal of Entomology |
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