Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16178 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil. |
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Silva, Camila Marques daChaves, Mariana MartinsRodrigues, Juliany ColaCorte-Real, SuzanaSilva, Robson CoutinhoPersechini, Pedro Muanis2016-10-11T11:34:34Z2016-10-11T11:34:34Z2011SILVA, Camila Marques da; et al. Differential Modulation of ATP-Induced P2X7-Associated Permeabilities to Cations and Anions of Macrophages by Infection with Leishmania amazonensis. Plos One, v.6, n.9, e25356, 10, Sept. 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/1617810.1371/journal.pone.0025356engPublic Library of ScienceLeishmania amazonensisMacrófagosP2X7 receptorCátionsÂnionsLeishmania amazonensisMacrophagesATP receptor P2X7CationsAnionsDifferential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil.Leishmania and other parasites display several mechanisms to subvert host immune cell function in order to achieve successful infection. The ATP receptor P2X7, an agonist-gated cation channel widely expressed in macrophages and other cells of the immune system, is also coupled to inflammasome activation, IL-1 beta secretion, production of reactive oxygen species, cell death and the induction of the permeabilization of the plasma membrane to molecules of up to 900 Da. P2X7 receptors can function as an effective microbicidal triggering receptor in macrophages infected with several microorganisms including Mycobacteria tuberculosis, Chlamydia and Leishmania. We have previously shown that its expression is up-regulated in macrophages infected with L. amazonensis and that infected cells also display an increase in P2X7-induced apoptosis and membrane permeabilization to some anionic fluorescent dyes. In an independent study we recently showed that the phenomenon of macrophage membrane permeabilization can involve at least two distinct pathways for cations and anions respectively. Here, we re-addressed the effects of ATP-induced P2X7-associated phenomena in macrophages infected with L. amazonensis and demonstrated that the P2X7-associated dye uptake mechanisms are differentially modulated. While the membrane permeabilization for anionic dyes is up-modulated, as previously described, the uptake of cationic dyes is strongly down-modulated. These results unveil new characteristics of two distinct permeabilization mechanisms associated with P2X7 receptors in macrophages and provide the first evidence indicating that these pathways can be differentially modulated in an immunologically relevant situation. The possible importance of these results to the L. amazonensis escape mechanism is discussed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16178/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALsuzana2_cortereal_etal_IOC_2011.pdfsuzana2_cortereal_etal_IOC_2011.pdfapplication/pdf1791768https://www.arca.fiocruz.br/bitstream/icict/16178/2/suzana2_cortereal_etal_IOC_2011.pdfbbf46ad75d79e2c3985488ddf122b3daMD52TEXTsuzana2_cortereal_etal_IOC_2011.pdf.txtsuzana2_cortereal_etal_IOC_2011.pdf.txtExtracted texttext/plain52599https://www.arca.fiocruz.br/bitstream/icict/16178/3/suzana2_cortereal_etal_IOC_2011.pdf.txt7a49eb3f53c2dcf435105257b23b1514MD53icict/161782018-04-02 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| dc.title.pt_BR.fl_str_mv |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| title |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| spellingShingle |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis Silva, Camila Marques da Leishmania amazonensis Macrófagos P2X7 receptor Cátions Ânions Leishmania amazonensis Macrophages ATP receptor P2X7 Cations Anions |
| title_short |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| title_full |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| title_fullStr |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| title_full_unstemmed |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| title_sort |
Differential modulation of ATP-induced P2X7-associated permeabilities to cations and anions of macrophages by infection with Leishmania amazonensis |
| author |
Silva, Camila Marques da |
| author_facet |
Silva, Camila Marques da Chaves, Mariana Martins Rodrigues, Juliany Cola Corte-Real, Suzana Silva, Robson Coutinho Persechini, Pedro Muanis |
| author_role |
author |
| author2 |
Chaves, Mariana Martins Rodrigues, Juliany Cola Corte-Real, Suzana Silva, Robson Coutinho Persechini, Pedro Muanis |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Camila Marques da Chaves, Mariana Martins Rodrigues, Juliany Cola Corte-Real, Suzana Silva, Robson Coutinho Persechini, Pedro Muanis |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania amazonensis Macrófagos P2X7 receptor Cátions Ânions |
| topic |
Leishmania amazonensis Macrófagos P2X7 receptor Cátions Ânions Leishmania amazonensis Macrophages ATP receptor P2X7 Cations Anions |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania amazonensis Macrophages ATP receptor P2X7 Cations Anions |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil. |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011 |
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2016-10-11T11:34:34Z |
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2016-10-11T11:34:34Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SILVA, Camila Marques da; et al. Differential Modulation of ATP-Induced P2X7-Associated Permeabilities to Cations and Anions of Macrophages by Infection with Leishmania amazonensis. Plos One, v.6, n.9, e25356, 10, Sept. 2011. |
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https://www.arca.fiocruz.br/handle/icict/16178 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0025356 |
| identifier_str_mv |
SILVA, Camila Marques da; et al. Differential Modulation of ATP-Induced P2X7-Associated Permeabilities to Cations and Anions of Macrophages by Infection with Leishmania amazonensis. Plos One, v.6, n.9, e25356, 10, Sept. 2011. 1932-6203 10.1371/journal.pone.0025356 |
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