Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing

Detalhes bibliográficos
Autor(a) principal: Monteiro, Carolina Cunha
Data de Publicação: 2016
Outros Autores: Villegas, Luis Eduardo Martinez, Campolina, Thais Bonifácio, Pires, Ana Clara Machado Araújo, Miranda, Jose Carlos, Pimenta, Paulo Filemon Paolucci, Secundino, Nagila Francinete Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16507
Resumo: CNPq. CCM, TBC and ACMAP are students, supported by CAPES and LEMV is supported by CNPq.
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spelling Monteiro, Carolina CunhaVillegas, Luis Eduardo MartinezCampolina, Thais BonifácioPires, Ana Clara Machado AraújoMiranda, Jose CarlosPimenta, Paulo Filemon PaolucciSecundino, Nagila Francinete Costa2016-12-12T12:58:10Z2016-12-12T12:58:10Z2016MONTEIRO, C. C. et al. Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing. Parasites & Vectors, v. 9, p. 480, 2016.1756-3305https://www.arca.fiocruz.br/handle/icict/1650710.1186/s13071-016-1767-zCNPq. CCM, TBC and ACMAP are students, supported by CAPES and LEMV is supported by CNPq.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, BrasilParasites of the genus Leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. American cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including Lutzomyia intermedia, a proven vector. The female sand fly can acquire or deliver Leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. The microbiota composition and abundance varies by food source, life stages and physiological conditions. The sand fly microbiota can affect parasite life-cycle in the vector. Methods: We performed a metagenomic analysis for microbiota composition and abundance in Lu. intermedia, from an endemic area in Brazil. The adult insects were collected using CDC light traps, morphologically identified, carefully sterilized, dissected under a microscope and the females separated into groups according to their physiological condition: (i) absence of blood meal (unfed = UN); (ii) presence of blood meal (blood-fed = BF); and (iii) presence of developed ovaries (gravid = GR). Then, they were processed for metagenomics with Illumina Hiseq Sequencing in order to be sequence analyzed and to obtain the taxonomic profiles of the microbiota. Results: Bacterial metagenomic analysis revealed differences in microbiota composition based upon the distinct physiological stages of the adult insect. Sequence identification revealed two phyla (Proteobacteria and Actinobacteria), 11 families and 15 genera; 87 % of the bacteria were Gram-negative, while only one family and two genera were identified as Gram-positive. The genera Ochrobactrum, Bradyrhizobium and Pseudomonas were found across all of the groups. Conclusions: The metagenomic analysis revealed that the microbiota of the Lu. intermedia female sand flies are distinct under specific physiological conditions and consist of 15 bacterial genera. The Ochrobactrum, Bradyrhizobium and Pseudomonas were the common genera. Our results detailing the constituents of Lu. intermedia native microbiota contribute to the knowledge regarding the bacterial community in an important sand fly vector and allow for further studies to better understand how the microbiota interacts with vectors of human parasites and to develop tools for biological control.engBioMed CentralLutzomyia intermediaMicrobiota nativaMetagenômicaLutzomyia intermediaNative microbiotaMetagenomicsBacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16507/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMonteiro CC Bacterial diversity.,...pdfMonteiro CC Bacterial 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dc.title.pt_BR.fl_str_mv Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
title Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
spellingShingle Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
Monteiro, Carolina Cunha
Lutzomyia intermedia
Microbiota nativa
Metagenômica
Lutzomyia intermedia
Native microbiota
Metagenomics
title_short Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
title_full Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
title_fullStr Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
title_full_unstemmed Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
title_sort Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing
author Monteiro, Carolina Cunha
author_facet Monteiro, Carolina Cunha
Villegas, Luis Eduardo Martinez
Campolina, Thais Bonifácio
Pires, Ana Clara Machado Araújo
Miranda, Jose Carlos
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
author_role author
author2 Villegas, Luis Eduardo Martinez
Campolina, Thais Bonifácio
Pires, Ana Clara Machado Araújo
Miranda, Jose Carlos
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Monteiro, Carolina Cunha
Villegas, Luis Eduardo Martinez
Campolina, Thais Bonifácio
Pires, Ana Clara Machado Araújo
Miranda, Jose Carlos
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
dc.subject.other.pt_BR.fl_str_mv Lutzomyia intermedia
Microbiota nativa
Metagenômica
topic Lutzomyia intermedia
Microbiota nativa
Metagenômica
Lutzomyia intermedia
Native microbiota
Metagenomics
dc.subject.en.pt_BR.fl_str_mv Lutzomyia intermedia
Native microbiota
Metagenomics
description CNPq. CCM, TBC and ACMAP are students, supported by CAPES and LEMV is supported by CNPq.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-12T12:58:10Z
dc.date.available.fl_str_mv 2016-12-12T12:58:10Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MONTEIRO, C. C. et al. Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing. Parasites & Vectors, v. 9, p. 480, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16507
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-016-1767-z
identifier_str_mv MONTEIRO, C. C. et al. Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing. Parasites & Vectors, v. 9, p. 480, 2016.
1756-3305
10.1186/s13071-016-1767-z
url https://www.arca.fiocruz.br/handle/icict/16507
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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