Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder

Detalhes bibliográficos
Autor(a) principal: Justino, Mariana Chantre
Data de Publicação: 2015
Outros Autores: Alves, Gilda, Britto, Constança, Cardoso, Angélica, Scherrer, Luciano, Moreira, Aline dos Santos, Quirino, Raul, Ornellas, Antonio, Leitão, Alvaro, Lage, Claudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13543
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil.
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spelling Justino, Mariana ChantreAlves, GildaBritto, ConstançaCardoso, AngélicaScherrer, LucianoMoreira, Aline dos SantosQuirino, RaulOrnellas, AntonioLeitão, AlvaroLage, Claudia2016-04-05T17:38:32Z2016-04-05T17:38:32Z2015JUSTINO, Mariana Chantre; et al. Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder. Oncology Reports, v.34, n.4, p.1667-1674, Oct. 2015.1021-335Xhttps://www.arca.fiocruz.br/handle/icict/1354310.3892/or.2015.41511791-2431engSpandidos PublicationsImpact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladderinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Coordenação de Pesquisa. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Sociedade Brasileira de Oncologia Clínica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. PDTIS Plataforma de Sequenciamento de DNA. Brasil.Instituto Nacional de Câncer. Departamento de Urologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Departamento de Urologia. Rio de Janeiro, RJ, Brasil / Hospital Mário Kröeff. Departamento de Urologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Abstract Molecular evidence indicates that alterations in genes involved in the maintenance of genome stability may be related to susceptibility to bladder carcinoma. Our goal was to evaluate the prognostic role of base excision repair (BER) genes in a cohort of patients diagnosed with primary urothelial carcinoma of the bladder (UCB). The levels of all APE1, XRCC1 and POLB transcripts were detected by quantitative real-time PCR (qPCR) technique in tumor samples from 52 patients undergoing transurethral resection (TUR) for primary UCB at the Department of Urology, Brazilian National Cancer Institute, Rio de Janeiro. Increased levels of APE1, XRCC1 and POLB transcripts were significantly associated with high-grade tumors when compared to these levels in low-grade tumors (p<0.01) and could be attributed to different mechanisms of transcriptional regulation as a response to tumorigenesis and oxidative stress. By analyzing the collected data in the present study, regardless of pathological grade or stage, univariate analysis revealed that the reduced levels of APE1 transcripts were significantly associated with cancer-specific mortality (p=0.032). Furthermore, the variant genotype (TG/GG) of the APE1 T1349G polymorphism was observed in 75% of a subset of patients who concomitantly experienced reduced levels of the APE1 transcript and death and/or recurrence events. Taken together, our data reinforce the idea that human DNA repair mechanisms must be finely regulated in order to avoid instability leading to tumorigenesis and poor clinical outcomes in UCB patients.Urothelial carcinomaBladderBexiga UrináriaCâncerinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13543/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALangelica_cardoso_etal_IOC_2015.pdfangelica_cardoso_etal_IOC_2015.pdfapplication/pdf669376https://www.arca.fiocruz.br/bitstream/icict/13543/2/angelica_cardoso_etal_IOC_2015.pdfbd50c616ae2a51797812897231893d1dMD52TEXTangelica_cardoso_etal_IOC_2015.pdf.txtangelica_cardoso_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
title Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
spellingShingle Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
Justino, Mariana Chantre
Urothelial carcinoma
Bladder
Bexiga Urinária
Câncer
title_short Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
title_full Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
title_fullStr Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
title_full_unstemmed Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
title_sort Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
author Justino, Mariana Chantre
author_facet Justino, Mariana Chantre
Alves, Gilda
Britto, Constança
Cardoso, Angélica
Scherrer, Luciano
Moreira, Aline dos Santos
Quirino, Raul
Ornellas, Antonio
Leitão, Alvaro
Lage, Claudia
author_role author
author2 Alves, Gilda
Britto, Constança
Cardoso, Angélica
Scherrer, Luciano
Moreira, Aline dos Santos
Quirino, Raul
Ornellas, Antonio
Leitão, Alvaro
Lage, Claudia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Justino, Mariana Chantre
Alves, Gilda
Britto, Constança
Cardoso, Angélica
Scherrer, Luciano
Moreira, Aline dos Santos
Quirino, Raul
Ornellas, Antonio
Leitão, Alvaro
Lage, Claudia
dc.subject.en.pt_BR.fl_str_mv Urothelial carcinoma
Bladder
topic Urothelial carcinoma
Bladder
Bexiga Urinária
Câncer
dc.subject.decs.pt_BR.fl_str_mv Bexiga Urinária
Câncer
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-05T17:38:32Z
dc.date.available.fl_str_mv 2016-04-05T17:38:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv JUSTINO, Mariana Chantre; et al. Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder. Oncology Reports, v.34, n.4, p.1667-1674, Oct. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13543
dc.identifier.issn.none.fl_str_mv 1021-335X
dc.identifier.doi.none.fl_str_mv 10.3892/or.2015.4151
dc.identifier.eissn.none.fl_str_mv 1791-2431
identifier_str_mv JUSTINO, Mariana Chantre; et al. Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder. Oncology Reports, v.34, n.4, p.1667-1674, Oct. 2015.
1021-335X
10.3892/or.2015.4151
1791-2431
url https://www.arca.fiocruz.br/handle/icict/13543
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Spandidos Publications
publisher.none.fl_str_mv Spandidos Publications
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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