A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations

Andrade, Ana Cláudia dos Santos Pereira; Silva, Gabriel Henrique Campolina; Queiroz Junior, Celso Martins; Oliveira, Leonardo Camilo de; Lacerda, Larisse de Souza Barbosa; Gaggino, Jordane Clarisse Pimenta; Souza, Filipe Resende Oliveira de; Chaves, Ian de Meira; Passos, Ingredy Beatriz; Teixeira, Danielle Cunha; Silva, Paloma Graziele Bittencourt; Valadão, Priscila Aparecida Costa; Oliveira, Leonardo Rossi; Antunes, Maisa Mota; Figueiredo, André Felipe Almeida; Wnuk, Natália Teixeira; Temerozo, Jairo Ramos; Ferreira, André Costa; Cramer, Allysson; Oliveira, Cleida Aparecida; Carvalho, Ricardo Durães; Arns, Clarice Weis; Guimarães, Pedro Pires Goulart; Costa, Guilherme Mattos Jardim; Menezes, Gustavo Batista de; Guatimosim, Cristina; Silva, Glauber Santos Ferreira da; Souza, Thiago Moreno L.; Barrioni, Breno Rocha; Pereira, Marivalda de Magalhães; Sousa, Lirlândia Pires de; Teixeira, Mauro Martins; Costa, Vivian Vasconcelos

A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations

Detalhes bibliográficos
Autor(a) principal:	Andrade, Ana Cláudia dos Santos Pereira
Data de Publicação:	2021
Outros Autores:	Silva, Gabriel Henrique Campolina, Queiroz Junior, Celso Martins, Oliveira, Leonardo Camilo de, Lacerda, Larisse de Souza Barbosa, Gaggino, Jordane Clarisse Pimenta, Souza, Filipe Resende Oliveira de, Chaves, Ian de Meira, Passos, Ingredy Beatriz, Teixeira, Danielle Cunha, Silva, Paloma Graziele Bittencourt, Valadão, Priscila Aparecida Costa, Oliveira, Leonardo Rossi, Antunes, Maisa Mota, Figueiredo, André Felipe Almeida, Wnuk, Natália Teixeira, Temerozo, Jairo Ramos, Ferreira, André Costa, Cramer, Allysson, Oliveira, Cleida Aparecida, Carvalho, Ricardo Durães, Arns, Clarice Weis, Guimarães, Pedro Pires Goulart, Costa, Guilherme Mattos Jardim, Menezes, Gustavo Batista de, Guatimosim, Cristina, Silva, Glauber Santos Ferreira da, Souza, Thiago Moreno L., Barrioni, Breno Rocha, Pereira, Marivalda de Magalhães, Sousa, Lirlândia Pires de, Teixeira, Mauro Martins, Costa, Vivian Vasconcelos
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/50746
Resumo:	Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.

Metadados do item

id	CRUZ_6ca0fbc682f773b08300049b43907199
oai_identifier_str	oai:www.arca.fiocruz.br:icict/50746
network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Andrade, Ana Cláudia dos Santos PereiraSilva, Gabriel Henrique CampolinaQueiroz Junior, Celso MartinsOliveira, Leonardo Camilo deLacerda, Larisse de Souza BarbosaGaggino, Jordane Clarisse PimentaSouza, Filipe Resende Oliveira deChaves, Ian de MeiraPassos, Ingredy BeatrizTeixeira, Danielle CunhaSilva, Paloma Graziele BittencourtValadão, Priscila Aparecida CostaOliveira, Leonardo RossiAntunes, Maisa MotaFigueiredo, André Felipe AlmeidaWnuk, Natália TeixeiraTemerozo, Jairo RamosFerreira, André CostaCramer, AllyssonOliveira, Cleida AparecidaCarvalho, Ricardo DurãesArns, Clarice WeisGuimarães, Pedro Pires GoulartCosta, Guilherme Mattos JardimMenezes, Gustavo Batista deGuatimosim, CristinaSilva, Glauber Santos Ferreira daSouza, Thiago Moreno L.Barrioni, Breno RochaPereira, Marivalda de MagalhãesSousa, Lirlândia Pires deTeixeira, Mauro MartinsCosta, Vivian Vasconcelos2022-01-13T12:56:44Z2022-01-13T12:56:44Z2021ANDRADE, Ana Cláudia dos SantosPereira et al. A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations. Journal of Virology, v. 95, n. 22, e01276-21, p. 1-18, Nov. 2021.1098-5514https://www.arca.fiocruz.br/handle/icict/5074610.1128/JVI.01276-210022-538XengAmerican Society for MicrobiologyBetacoronavírusMHV-3Infecção pulmonarCOVID-19Modelo animalFator de necrose tumoralBetacoronavirusMHV-3Lung infectionCOVID-19Animal modelTumor necrosis factorA Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia em Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Laboratório de Pesquisas Pre-Clínicas. Nova Iguaçu, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Estadual de Campinas, Laboratório de Virologia. Campinas, SP, Brasil.Universidade Estadual de Campinas, Laboratório de Virologia. Campinas, SP, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Escola de Engenharia. Departamento de Engenharia Metalúrgica e Materiais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Engenharia. Departamento de Engenharia Metalúrgica e Materiais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.The emergence of life-threatening zoonotic diseases caused by betacoronaviruses, including the ongoing coronavirus disease 19 (COVID-19) pandemic, has highlighted the need for developing preclinical models mirroring respiratory and systemic pathophysiological manifestations seen in infected humans. Here, we showed that C57BL/6J wild-type mice intranasally inoculated with the murine betacoronavirus murine hepatitis coronavirus 3 (MHV-3) develop a robust inflammatory response leading to acute lung injuries, including alveolar edema, hemorrhage, and fibrin thrombi. Although such histopathological changes seemed to resolve as the infection advanced, they efficiently impaired respiratory function, as the infected mice displayed restricted lung distention and increased respiratory frequency and ventilation. Following respiratory manifestation, the MHV-3 infection became systemic, and a high virus burden could be detected in multiple organs along with morphological changes. The systemic manifestation of MHV-3 infection was also marked by a sharp drop in the number of circulating platelets and lymphocytes, besides the augmented concentration of the proinflammatory cytokines interleukin 1 beta (IL-1b), IL-6, IL-12, gamma interferon (IFN-g), and tumor necrosis factor (TNF), thereby mirroring some clinical features observed in moderate and severe cases of COVID-19. Importantly, both respiratory and systemic changes triggered by MHV-3 infection were greatly prevented by blocking TNF signaling, either via genetic or pharmacologic approaches. In line with this, TNF blockage also diminished the infection- mediated release of proinflammatory cytokines and virus replication of human epithelial lung cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Collectively, results show that MHV-3 respiratory infection leads to a large range of clinical manifestations in mice and may constitute an attractive, lower-cost, biosafety level 2 (BSL2) in vivo platform for evaluating the respiratory and multiorgan involvement of betacoronavirus infections. IMPORTANCE Mouse models have long been used as valuable in vivo platforms to investigate the pathogenesis of viral infections and effective countermeasures. The natural resistance of mice to the novel betacoronavirus SARS-CoV-2, the causative agent of COVID-19, has launched a race toward the characterization of SARS-CoV-2 infection in other animals (e.g., hamsters, cats, ferrets, bats, and monkeys), as well as adaptation of the mouse model, by modifying either the host or the virus. In the present study, we utilized a natural pathogen of mice, MHV, as a prototype to model betacoronavirus-induced acute lung injure and multiorgan involvement under biosafety level 2 conditions. We showed that C57BL/6J mice intranasally inoculated with MHV-3 develops severe disease, which includes acute lung damage and respiratory distress that precede systemic inflammation and death. Accordingly, the proposed animal model may provide a useful tool for studies regarding betacoronavirus respiratory infection and related diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALJaime_Temerozo_etal_IOC_2021.pdfJaime_Temerozo_etal_IOC_2021.pdfapplication/pdf7900929https://www.arca.fiocruz.br/bitstream/icict/50746/2/Jaime_Temerozo_etal_IOC_2021.pdf48c0d800e292f2c055dc1db1a34f05c5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50746/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/507462023-02-13 14:15:42.339oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-02-13T17:15:42Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
title	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
spellingShingle	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations Andrade, Ana Cláudia dos Santos Pereira Betacoronavírus MHV-3 Infecção pulmonar COVID-19 Modelo animal Fator de necrose tumoral Betacoronavirus MHV-3 Lung infection COVID-19 Animal model Tumor necrosis factor
title_short	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
title_full	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
title_fullStr	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
title_full_unstemmed	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
title_sort	A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations
author	Andrade, Ana Cláudia dos Santos Pereira
author_facet	Andrade, Ana Cláudia dos Santos Pereira Silva, Gabriel Henrique Campolina Queiroz Junior, Celso Martins Oliveira, Leonardo Camilo de Lacerda, Larisse de Souza Barbosa Gaggino, Jordane Clarisse Pimenta Souza, Filipe Resende Oliveira de Chaves, Ian de Meira Passos, Ingredy Beatriz Teixeira, Danielle Cunha Silva, Paloma Graziele Bittencourt Valadão, Priscila Aparecida Costa Oliveira, Leonardo Rossi Antunes, Maisa Mota Figueiredo, André Felipe Almeida Wnuk, Natália Teixeira Temerozo, Jairo Ramos Ferreira, André Costa Cramer, Allysson Oliveira, Cleida Aparecida Carvalho, Ricardo Durães Arns, Clarice Weis Guimarães, Pedro Pires Goulart Costa, Guilherme Mattos Jardim Menezes, Gustavo Batista de Guatimosim, Cristina Silva, Glauber Santos Ferreira da Souza, Thiago Moreno L. Barrioni, Breno Rocha Pereira, Marivalda de Magalhães Sousa, Lirlândia Pires de Teixeira, Mauro Martins Costa, Vivian Vasconcelos
author_role	author
author2	Silva, Gabriel Henrique Campolina Queiroz Junior, Celso Martins Oliveira, Leonardo Camilo de Lacerda, Larisse de Souza Barbosa Gaggino, Jordane Clarisse Pimenta Souza, Filipe Resende Oliveira de Chaves, Ian de Meira Passos, Ingredy Beatriz Teixeira, Danielle Cunha Silva, Paloma Graziele Bittencourt Valadão, Priscila Aparecida Costa Oliveira, Leonardo Rossi Antunes, Maisa Mota Figueiredo, André Felipe Almeida Wnuk, Natália Teixeira Temerozo, Jairo Ramos Ferreira, André Costa Cramer, Allysson Oliveira, Cleida Aparecida Carvalho, Ricardo Durães Arns, Clarice Weis Guimarães, Pedro Pires Goulart Costa, Guilherme Mattos Jardim Menezes, Gustavo Batista de Guatimosim, Cristina Silva, Glauber Santos Ferreira da Souza, Thiago Moreno L. Barrioni, Breno Rocha Pereira, Marivalda de Magalhães Sousa, Lirlândia Pires de Teixeira, Mauro Martins Costa, Vivian Vasconcelos
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Andrade, Ana Cláudia dos Santos Pereira Silva, Gabriel Henrique Campolina Queiroz Junior, Celso Martins Oliveira, Leonardo Camilo de Lacerda, Larisse de Souza Barbosa Gaggino, Jordane Clarisse Pimenta Souza, Filipe Resende Oliveira de Chaves, Ian de Meira Passos, Ingredy Beatriz Teixeira, Danielle Cunha Silva, Paloma Graziele Bittencourt Valadão, Priscila Aparecida Costa Oliveira, Leonardo Rossi Antunes, Maisa Mota Figueiredo, André Felipe Almeida Wnuk, Natália Teixeira Temerozo, Jairo Ramos Ferreira, André Costa Cramer, Allysson Oliveira, Cleida Aparecida Carvalho, Ricardo Durães Arns, Clarice Weis Guimarães, Pedro Pires Goulart Costa, Guilherme Mattos Jardim Menezes, Gustavo Batista de Guatimosim, Cristina Silva, Glauber Santos Ferreira da Souza, Thiago Moreno L. Barrioni, Breno Rocha Pereira, Marivalda de Magalhães Sousa, Lirlândia Pires de Teixeira, Mauro Martins Costa, Vivian Vasconcelos
dc.subject.other.pt_BR.fl_str_mv	Betacoronavírus MHV-3 Infecção pulmonar COVID-19 Modelo animal Fator de necrose tumoral
topic	Betacoronavírus MHV-3 Infecção pulmonar COVID-19 Modelo animal Fator de necrose tumoral Betacoronavirus MHV-3 Lung infection COVID-19 Animal model Tumor necrosis factor
dc.subject.en.pt_BR.fl_str_mv	Betacoronavirus MHV-3 Lung infection COVID-19 Animal model Tumor necrosis factor
description	Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.
publishDate	2021
dc.date.issued.fl_str_mv	2021
dc.date.accessioned.fl_str_mv	2022-01-13T12:56:44Z
dc.date.available.fl_str_mv	2022-01-13T12:56:44Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	ANDRADE, Ana Cláudia dos SantosPereira et al. A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations. Journal of Virology, v. 95, n. 22, e01276-21, p. 1-18, Nov. 2021.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/50746
dc.identifier.issn.pt_BR.fl_str_mv	1098-5514
dc.identifier.doi.none.fl_str_mv	10.1128/JVI.01276-21
dc.identifier.eissn.none.fl_str_mv	0022-538X
identifier_str_mv	ANDRADE, Ana Cláudia dos SantosPereira et al. A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations. Journal of Virology, v. 95, n. 22, e01276-21, p. 1-18, Nov. 2021. 1098-5514 10.1128/JVI.01276-21 0022-538X
url	https://www.arca.fiocruz.br/handle/icict/50746
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	American Society for Microbiology
publisher.none.fl_str_mv	American Society for Microbiology
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/50746/2/Jaime_Temerozo_etal_IOC_2021.pdf https://www.arca.fiocruz.br/bitstream/icict/50746/3/license.txt
bitstream.checksum.fl_str_mv	48c0d800e292f2c055dc1db1a34f05c5 5a560609d32a3863062d77ff32785d58
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
_version_	1798324649116827648

A Biosafety Level 2 Mouse Model for Studying Betacoronavirus- Induced Acute Lung Damage and Systemic Manifestations

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