Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections

Detalhes bibliográficos
Autor(a) principal: Coeli, Regina
Data de Publicação: 2013
Outros Autores: Baba, Elio Hideo, Araujo, Neusa, Coelho, Paulo Marcos Zech, Oliveira, Guilherme Corrêa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29982
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brazil
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spelling Coeli, ReginaBaba, Elio HideoAraujo, NeusaCoelho, Paulo Marcos ZechOliveira, Guilherme Corrêa de2018-11-12T12:10:52Z2018-11-12T12:10:52Z2013COELI, Regina et al. Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections. PLoS Negl Trop Dis., v. 7, n. 12, e2596, 2013.1935-2735https://www.arca.fiocruz.br/handle/icict/2998210.1371/journal.pntd.0002596engPublic Library of ScienceEsquistossomosseSchistosomiasisPraziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou.Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, MG, BrazilBackground: Schistosomiasis has a considerable impact on public health in many tropical and subtropical areas. In the new world, schistosomiasis is caused by the digenetic trematode Schistosoma mansoni. Chemotherapy is the main measure for controlling schistosomiasis, and the current drug of choice for treatment is praziquantel (PZQ). Although PZQ is efficient and safe, its repetitive large-scale use in endemic areas may lead to the selection of resistant strains. Isolates less susceptible to PZQ have been found in the field and selected for in the laboratory. The impact of selecting strains with a decreased susceptibility phenotype on disease dynamics and parasite population genetics is not fully understood. This study addresses the impact of PZQ pressure on the genetics of a laboratory population by analyzing frequency variations of polymorphic genetic markers. Methodology: Infected mice were treated with increasing PZQ doses until the highest dose of 3×300 mg/Kg was reached. The effect of PZQ treatment on the parasite population was assessed using five polymorphic microsatellite markers. Parasitological and genetic data were compared with those of the untreated control. After six parasite generations submitted to treatment, it was possible to obtain a S. mansoni population with decreased susceptibility to PZQ. In our experiments we also observed that female worms were more susceptible to PZQ than male worms. Conclusions: The selective pressure exerted by PZQ led to decreased genetic variability in S. mansoni and increased endogamy. The understanding of how S. mansoni populations respond to successive drug pressure has important implications on the appearance and maintenance of a PZQ resistance phenotype in endemic regions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/29982/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPraziquantel treatment decreases Schistosoma mansoni.pdfPraziquantel treatment decreases Schistosoma mansoni.pdfapplication/pdf3889503https://www.arca.fiocruz.br/bitstream/icict/29982/2/Praziquantel%20treatment%20decreases%20Schistosoma%20mansoni.pdf5c3394762f1d0aa939208c4c7d9bd191MD52TEXTPraziquantel treatment decreases Schistosoma mansoni.pdf.txtPraziquantel treatment decreases Schistosoma mansoni.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
title Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
spellingShingle Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
Coeli, Regina
Esquistossomosse
Schistosomiasis
title_short Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
title_full Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
title_fullStr Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
title_full_unstemmed Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
title_sort Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections
author Coeli, Regina
author_facet Coeli, Regina
Baba, Elio Hideo
Araujo, Neusa
Coelho, Paulo Marcos Zech
Oliveira, Guilherme Corrêa de
author_role author
author2 Baba, Elio Hideo
Araujo, Neusa
Coelho, Paulo Marcos Zech
Oliveira, Guilherme Corrêa de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Coeli, Regina
Baba, Elio Hideo
Araujo, Neusa
Coelho, Paulo Marcos Zech
Oliveira, Guilherme Corrêa de
dc.subject.other.pt_BR.fl_str_mv Esquistossomosse
topic Esquistossomosse
Schistosomiasis
dc.subject.en.pt_BR.fl_str_mv Schistosomiasis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brazil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-11-12T12:10:52Z
dc.date.available.fl_str_mv 2018-11-12T12:10:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COELI, Regina et al. Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections. PLoS Negl Trop Dis., v. 7, n. 12, e2596, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29982
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0002596
identifier_str_mv COELI, Regina et al. Praziquantel Treatment Decreases Schistosoma mansoni Genetic Diversity in Experimental Infections. PLoS Negl Trop Dis., v. 7, n. 12, e2596, 2013.
1935-2735
10.1371/journal.pntd.0002596
url https://www.arca.fiocruz.br/handle/icict/29982
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/29982/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/29982/2/Praziquantel%20treatment%20decreases%20Schistosoma%20mansoni.pdf
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