DNA extraction from fixed cytogenetic cell suspensions

Detalhes bibliográficos
Autor(a) principal: Amorim, M. R.
Data de Publicação: 2007
Outros Autores: Vargas, F. R., Llerena Junior, Juan Clinton, Pombo-de-Oliveira, M. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10749
Resumo: Instituto Nacional de Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil.
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spelling Amorim, M. R.Vargas, F. R.Llerena Junior, Juan ClintonPombo-de-Oliveira, M. S.2015-06-09T16:41:43Z2015-06-09T16:41:43Z2007AMORIM, M. R. et al. DNA extraction from fixed cytogenetic cell suspensions. Genetics and Molecular Research, Ribeirao Preto, v. 6, n. 3, p. 500-503, 2007.1676-5680https://www.arca.fiocruz.br/handle/icict/10749engFundação Norte-Rio-Grandense de Pesquisa e CulturaDNA extraction from fixed cytogenetic cell suspensionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil. / Universidade Federal do Estado do Rio de Janeiro. Unidade de Genética. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Divisão de Genética. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil.AbstRACt. We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for con-ventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.DNA ExtractionDNAzolCell SuspensionCytogeneticDNACitogenéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10749/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALDNA extraction from fixed cytogenetic cell suspensions.pdfDNA extraction from fixed cytogenetic cell suspensions.pdfapplication/pdf280083https://www.arca.fiocruz.br/bitstream/icict/10749/2/DNA%20extraction%20from%20fixed%20cytogenetic%20cell%20suspensions.pdfb8e98f0834cbb8fbffe65f1bbea4b120MD52TEXTDNA extraction from fixed cytogenetic cell suspensions.pdf.txtDNA extraction from fixed cytogenetic cell suspensions.pdf.txtExtracted texttext/plain8961https://www.arca.fiocruz.br/bitstream/icict/10749/3/DNA%20extraction%20from%20fixed%20cytogenetic%20cell%20suspensions.pdf.txt3ded1f95437ed3884586142bcba30a0cMD53icict/107492020-05-12 13:23:21.796oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-05-12T16:23:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv DNA extraction from fixed cytogenetic cell suspensions
title DNA extraction from fixed cytogenetic cell suspensions
spellingShingle DNA extraction from fixed cytogenetic cell suspensions
Amorim, M. R.
DNA Extraction
DNAzol
Cell Suspension
Cytogenetic
DNA
Citogenética
title_short DNA extraction from fixed cytogenetic cell suspensions
title_full DNA extraction from fixed cytogenetic cell suspensions
title_fullStr DNA extraction from fixed cytogenetic cell suspensions
title_full_unstemmed DNA extraction from fixed cytogenetic cell suspensions
title_sort DNA extraction from fixed cytogenetic cell suspensions
author Amorim, M. R.
author_facet Amorim, M. R.
Vargas, F. R.
Llerena Junior, Juan Clinton
Pombo-de-Oliveira, M. S.
author_role author
author2 Vargas, F. R.
Llerena Junior, Juan Clinton
Pombo-de-Oliveira, M. S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Amorim, M. R.
Vargas, F. R.
Llerena Junior, Juan Clinton
Pombo-de-Oliveira, M. S.
dc.subject.en.pt_BR.fl_str_mv DNA Extraction
DNAzol
Cell Suspension
Cytogenetic
topic DNA Extraction
DNAzol
Cell Suspension
Cytogenetic
DNA
Citogenética
dc.subject.decs.pt_BR.fl_str_mv DNA
Citogenética
description Instituto Nacional de Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2015-06-09T16:41:43Z
dc.date.available.fl_str_mv 2015-06-09T16:41:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AMORIM, M. R. et al. DNA extraction from fixed cytogenetic cell suspensions. Genetics and Molecular Research, Ribeirao Preto, v. 6, n. 3, p. 500-503, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10749
dc.identifier.issn.none.fl_str_mv 1676-5680
identifier_str_mv AMORIM, M. R. et al. DNA extraction from fixed cytogenetic cell suspensions. Genetics and Molecular Research, Ribeirao Preto, v. 6, n. 3, p. 500-503, 2007.
1676-5680
url https://www.arca.fiocruz.br/handle/icict/10749
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Norte-Rio-Grandense de Pesquisa e Cultura
publisher.none.fl_str_mv Fundação Norte-Rio-Grandense de Pesquisa e Cultura
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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