Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Pinto, Patricia Machado
Data de Publicação: 1997
Outros Autores: Brito, Cristiana F. A., Passos, Liana K. Jannoti, Tendler, Miriam, Simpson, Andrew J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36863
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil.
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spelling Pinto, Patricia MachadoBrito, Cristiana F. A.Passos, Liana K. JannotiTendler, MiriamSimpson, Andrew J. G.2019-11-05T13:43:05Z2019-11-05T13:43:05Z1997PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997.0074-0276https://www.arca.fiocruz.br/handle/icict/3686310.1590/s0074-027619970003000191678-9849engFundação Oswaldo Cruz. Instituto Oswaldo CruzSchistosoma mansoniReação em Cadeia da PolimeraseDNA polimórfico amplificado aleatoriamenteEsquistossomosePolymerase chain reactionRandomly amplified polymorphic DNASchistosomiasisSchistosoma mansoniContrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malacologia. Belo Horizonte. MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malacologia. Belo Horizonte. MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil.Instituto Ludwig de Pesquisas sobre o Câncer. Laboratório de Genética de Câncer. São Paulo, SP, Brasil.The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic DNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clones were constructed. The data showed that while the laboratory strain is highly homogeneous the clones derived from the field populations were highly variable with 43% of RAPDs exhibiting polymorphisms among 23 clones. Clones isolated from the same infected individual were always more closely grouped than clones from different individuals. The data clearly demonstrated that earlier analyses of the genomic variability in S. mansoni have underestimated this phenomenon due to the failure to examine field isolates.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36863/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPatriciaM_Pinto_etal_IOC_1997.pdfPatriciaM_Pinto_etal_IOC_1997.pdfapplication/pdf271575https://www.arca.fiocruz.br/bitstream/icict/36863/2/PatriciaM_Pinto_etal_IOC_1997.pdf111c5d8d7277866838054bc8191f2500MD52TEXTPatriciaM_Pinto_etal_IOC_1997.pdf.txtPatriciaM_Pinto_etal_IOC_1997.pdf.txtExtracted texttext/plain17197https://www.arca.fiocruz.br/bitstream/icict/36863/3/PatriciaM_Pinto_etal_IOC_1997.pdf.txtc5d55ff02d44c0d32061e9c3ebe7a7ceMD53icict/368632019-12-15 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dc.title.pt_BR.fl_str_mv Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
title Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
spellingShingle Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
Pinto, Patricia Machado
Schistosoma mansoni
Reação em Cadeia da Polimerase
DNA polimórfico amplificado aleatoriamente
Esquistossomose
Polymerase chain reaction
Randomly amplified polymorphic DNA
Schistosomiasis
Schistosoma mansoni
title_short Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
title_full Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
title_fullStr Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
title_full_unstemmed Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
title_sort Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
author Pinto, Patricia Machado
author_facet Pinto, Patricia Machado
Brito, Cristiana F. A.
Passos, Liana K. Jannoti
Tendler, Miriam
Simpson, Andrew J. G.
author_role author
author2 Brito, Cristiana F. A.
Passos, Liana K. Jannoti
Tendler, Miriam
Simpson, Andrew J. G.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pinto, Patricia Machado
Brito, Cristiana F. A.
Passos, Liana K. Jannoti
Tendler, Miriam
Simpson, Andrew J. G.
dc.subject.other.pt_BR.fl_str_mv Schistosoma mansoni
Reação em Cadeia da Polimerase
DNA polimórfico amplificado aleatoriamente
Esquistossomose
topic Schistosoma mansoni
Reação em Cadeia da Polimerase
DNA polimórfico amplificado aleatoriamente
Esquistossomose
Polymerase chain reaction
Randomly amplified polymorphic DNA
Schistosomiasis
Schistosoma mansoni
dc.subject.en.pt_BR.fl_str_mv Polymerase chain reaction
Randomly amplified polymorphic DNA
Schistosomiasis
Schistosoma mansoni
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil.
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2019-11-05T13:43:05Z
dc.date.available.fl_str_mv 2019-11-05T13:43:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36863
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02761997000300019
dc.identifier.eissn.none.fl_str_mv 1678-9849
identifier_str_mv PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997.
0074-0276
10.1590/s0074-02761997000300019
1678-9849
url https://www.arca.fiocruz.br/handle/icict/36863
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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