Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.

Detalhes bibliográficos
Autor(a) principal: Prim, Rodrigo Ivan
Data de Publicação: 2015
Outros Autores: Schörner, Marcos André, Senna, Simone Gonçalves, Nogueira, Christiane Lourenço, Figueiredo, Anna Carolina Cançado, Oliveira, Jaquelline Germano de, Rovaris, Darcita Bürger, Bazzo, Maria Luiza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12785
Resumo: Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, Brasil
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spelling Prim, Rodrigo IvanSchörner, Marcos AndréSenna, Simone GonçalvesNogueira, Christiane LourençoFigueiredo, Anna Carolina CançadoOliveira, Jaquelline Germano deRovaris, Darcita BürgerBazzo, Maria Luiza2016-02-22T18:06:23Z2016-02-22T18:06:23Z2015PRIM, Rodrigo Ivan et al. Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil. Mem Inst Oswaldo Cruz., vol. 110, n. 5, p. 618-623, 20151678-8060https://www.arca.fiocruz.br/handle/icict/1278510.1590/0074-02760150100engFundação Oswaldo CruzMolecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilLaboratório Central de Saúde Pública de Santa Catarina. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, BrasilDrug resistance is a global threat and one of the main contributing factors to tuberculosis (TB) outbreaks. The goal of this study was to analyse the molecular profile of multidrug-resistant TB (MDR-TB) in the state of Santa Catarina in southern Brazil. Fifty-three MDR Mycobacterium tuberculosis clinical isolates were analysed by spoligotyping and a partial region of the rpoB gene, which is associated with rifampicin resistance (RMP-R), was sequenced. Some isolates were also distinguished by their mycobacterial interspersed repetitive units (MIRU). S531L was the most prevalent mutation found within rpoB in RMP-R isolates (58.5%), followed by S531W (20.8%). Only two MDR isolates showed no mutations within rpoB. Isolates of the Latin American Mediterranean (LAM) family were the most prevalent (45.3%) found by spoligotyping, followed by Haarlem (9.4%) and T (7.5%) families. SIT106 was found in 26.4% of isolates and all SIT106 isolates typed by MIRU-12 (5 out of 14) belong to MIT251. There was a high correlation between the S531W mutation and the LAM family mainly because all SIT2263 (LAM9) isolates carry this mutation. Among isolates with the S531W mutation in rpoB MIRU demonstrates a cluster formed by four isolates (SIT2263 and MIT163) and very similar profiles were observed between eight of the nine isolates. Better characterisation of TB isolates may lead to new ways in which to control and treat TB in this region of Brazil.MDR-TB rpoBtuberculosisspoligotypingmolecular epidemiologyMIRUinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12785/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMolecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil..pdfMolecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil..pdfapplication/pdf292609https://www.arca.fiocruz.br/bitstream/icict/12785/2/Molecular%20profiling%20of%20drug%20resistant%20isolates%20of%20Mycobacterium%20tuberculosis%20in%20the%20state%20of%20Santa%20Catarina%2c%20southern%20Brazil..pdf4bea0aaa468b44fe8ec1bba4d8017a9eMD52TEXTMolecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil..pdf.txtMolecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil..pdf.txtExtracted texttext/plain31619https://www.arca.fiocruz.br/bitstream/icict/12785/3/Molecular%20profiling%20of%20drug%20resistant%20isolates%20of%20Mycobacterium%20tuberculosis%20in%20the%20state%20of%20Santa%20Catarina%2c%20southern%20Brazil..pdf.txtc603d845bf2891f6be934b022bfdebb2MD53icict/127852019-06-19 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dc.title.pt_BR.fl_str_mv Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
title Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
spellingShingle Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
Prim, Rodrigo Ivan
MDR-TB rpoB
tuberculosis
spoligotyping
molecular epidemiology
MIRU
title_short Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
title_full Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
title_fullStr Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
title_full_unstemmed Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
title_sort Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.
author Prim, Rodrigo Ivan
author_facet Prim, Rodrigo Ivan
Schörner, Marcos André
Senna, Simone Gonçalves
Nogueira, Christiane Lourenço
Figueiredo, Anna Carolina Cançado
Oliveira, Jaquelline Germano de
Rovaris, Darcita Bürger
Bazzo, Maria Luiza
author_role author
author2 Schörner, Marcos André
Senna, Simone Gonçalves
Nogueira, Christiane Lourenço
Figueiredo, Anna Carolina Cançado
Oliveira, Jaquelline Germano de
Rovaris, Darcita Bürger
Bazzo, Maria Luiza
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Prim, Rodrigo Ivan
Schörner, Marcos André
Senna, Simone Gonçalves
Nogueira, Christiane Lourenço
Figueiredo, Anna Carolina Cançado
Oliveira, Jaquelline Germano de
Rovaris, Darcita Bürger
Bazzo, Maria Luiza
dc.subject.en.pt_BR.fl_str_mv MDR-TB rpoB
tuberculosis
spoligotyping
molecular epidemiology
MIRU
topic MDR-TB rpoB
tuberculosis
spoligotyping
molecular epidemiology
MIRU
description Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis, SC, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-02-22T18:06:23Z
dc.date.available.fl_str_mv 2016-02-22T18:06:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PRIM, Rodrigo Ivan et al. Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil. Mem Inst Oswaldo Cruz., vol. 110, n. 5, p. 618-623, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12785
dc.identifier.issn.none.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760150100
identifier_str_mv PRIM, Rodrigo Ivan et al. Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil. Mem Inst Oswaldo Cruz., vol. 110, n. 5, p. 618-623, 2015
1678-8060
10.1590/0074-02760150100
url https://www.arca.fiocruz.br/handle/icict/12785
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12785/1/license.txt
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