Role of IL-4 in an experimental model of encephalitis induced by intracranial inoculation of herpes simplex virus-1 (HSV-1)

• Autor(a) principal: Vilela, Márcia Carvalho
• Data de Publicação: 2011
• Outros Autores: Campos, Roberta Dayrell de Lima, Mansur, Daniel Santos, Rodrigues, David Henrique, Queiroz, Norinne Lacerda, Lima, Graciela Kunrath, Rachid, Milene Alvarenga, Kroon, Erna Geessien, Campos, Marco Antônio, Teixeira, Antônio Lúcio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
• Texto Completo: https://www.arca.fiocruz.br/handle/icict/9371
• topic: herpes simplex; virus type 1; IL-4; neuroinflammation; vírus herpes; simplex tipo 1; IL-4; neuroinflamação

Herpes simplex virus-1 (HSV-1) is a pathogen that may cause severe encephalitis in humans. In this study, we aimed to investigate the role of interleukin-4 (IL-4) in a model of HSV-1 brain infection. IL-4 knockout (IL-4-/-) and wild type (WT) C57BL/6 mice were inoculated with 104 plaque-forming units of HSV-1 by the intracranial route. Histopathologic analysis revealed a distinct profile of infiltrating cells at 3 days post-infection (dpi). Infected WT mice presented mononuclear inflammatory cells while IL-4-/- mice developed meningoencephalitis with predominance of neutrophils. IL-4-/- mice had diminished leukocyte adhesion at 3 dpi when compared to infected WT animals in intravital microscopy study. Conversely no differences were found in cerebral levels of CXCL1, CXCL9, CCL3, CCL5 and TNF-α between WT and IL-4-/- infected mice. IL-4 may play a role in the recruitment of cells into central nervous system in this acute model of severe encephalitis caused by HSV-1.