Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation

Detalhes bibliográficos
Autor(a) principal: Marlow, Mariel A.
Data de Publicação: 2014
Outros Autores: Boité, Mariana C., Ferreira, Gabriel Eduardo M., Steindel, Mario, Cupolillo, Elisa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10697
Resumo: Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Protozoologia. Florianópolis, SC, Brasil.
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spelling Marlow, Mariel A.Boité, Mariana C.Ferreira, Gabriel Eduardo M.Steindel, MarioCupolillo, Elisa2015-06-08T14:01:52Z2015-06-08T14:01:52Z2014MARLOW, Mariel A. et al. Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation. PLOS Neglected Tropical Diseases, v. 8, n. 2, p. 1-10, 2014.1935-2727https://www.arca.fiocruz.br/handle/icict/1069710.1371/journal.pntd.0002695engPlos OneLieshmaniaBrasilSanta CatarinaLeishmania braziliensisMultilocus Sequence AnalysisBrazilMultilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Protozoologia. Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Protozoologia. Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.With the emergence of leishmaniasis in new regions around the world, molecular epidemiological methods with adequate discriminatory power, reproducibility, high throughput and inter-laboratory comparability are needed for outbreak investigation of this complex parasitic disease. As multilocus sequence analysis (MLSA) has been projected as the future gold standard technique for Leishmania species characterization, we propose a MLSA panel of six housekeeping gene loci (6pgd, mpi, icd, hsp70, mdhmt, mdhnc) for investigating intraspecific genetic variation of L. (Viannia) braziliensis strains and compare the resulting genetic clusters with several epidemiological factors relevant to outbreak investigation. The recent outbreak of cutaneous leishmaniasis caused by L. (V.) braziliensis in the southern Brazilian state of Santa Catarina is used to demonstrate the applicability of this technique. Sequenced fragments from six genetic markers from 86 L. (V.) braziliensis strains from twelve Brazilian states, including 33 strains from Santa Catarina, were used to determine clonal complexes, genetic structure, and phylogenic networks. Associations between genetic clusters and networks with epidemiological characteristics of patients were investigated. MLSA revealed epidemiological patterns among L. (V.) braziliensis strains, even identifying strains from imported cases among the Santa Catarina strains that presented extensive homogeneity. Evidence presented here has demonstrated MLSA possesses adequate discriminatory power for outbreak investigation, as well as other potential uses in the molecular epidemiology of leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10697/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALgabriel_ferreiraetal_IOC_2014.pdfapplication/pdf977537https://www.arca.fiocruz.br/bitstream/icict/10697/2/gabriel_ferreiraetal_IOC_2014.pdf2acfd4f7a06a742cadfb1642accb2c38MD52TEXTgabriel_ferreiraetal_IOC_2014.pdf.txtgabriel_ferreiraetal_IOC_2014.pdf.txtExtracted texttext/plain49569https://www.arca.fiocruz.br/bitstream/icict/10697/3/gabriel_ferreiraetal_IOC_2014.pdf.txtb7c935b4e77f50e4f4f718af1f4d7e4aMD53icict/106972022-06-24 12:19:39.996oai:www.arca.fiocruz.br:icict/10697TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:39Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
title Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
spellingShingle Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
Marlow, Mariel A.
Lieshmania
Brasil
Santa Catarina
Leishmania braziliensis
Multilocus Sequence Analysis
Brazil
title_short Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
title_full Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
title_fullStr Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
title_full_unstemmed Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
title_sort Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation
author Marlow, Mariel A.
author_facet Marlow, Mariel A.
Boité, Mariana C.
Ferreira, Gabriel Eduardo M.
Steindel, Mario
Cupolillo, Elisa
author_role author
author2 Boité, Mariana C.
Ferreira, Gabriel Eduardo M.
Steindel, Mario
Cupolillo, Elisa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Marlow, Mariel A.
Boité, Mariana C.
Ferreira, Gabriel Eduardo M.
Steindel, Mario
Cupolillo, Elisa
dc.subject.other.pt_BR.fl_str_mv Lieshmania
Brasil
Santa Catarina
topic Lieshmania
Brasil
Santa Catarina
Leishmania braziliensis
Multilocus Sequence Analysis
Brazil
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Multilocus Sequence Analysis
Brazil
description Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Protozoologia. Florianópolis, SC, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:52Z
dc.date.available.fl_str_mv 2015-06-08T14:01:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARLOW, Mariel A. et al. Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation. PLOS Neglected Tropical Diseases, v. 8, n. 2, p. 1-10, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10697
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0002695
identifier_str_mv MARLOW, Mariel A. et al. Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation. PLOS Neglected Tropical Diseases, v. 8, n. 2, p. 1-10, 2014.
1935-2727
10.1371/journal.pntd.0002695
url https://www.arca.fiocruz.br/handle/icict/10697
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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