Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests

Detalhes bibliográficos
Autor(a) principal: Teixeira, Ana Izabel Passarella
Data de Publicação: 2019
Outros Autores: Silva, Debora Marcolino, Vital, Tamires, Nitz, Nadjar, Carvalho, Bruna Caroline de, Hecht, Mariana, Oliveira, Diana Souza de, Oliveira, Edward Jose de, Rabello, Ana Lucia Teles, Romero, Gustavo Adolfo Sierra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39799
Resumo: Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.
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spelling Teixeira, Ana Izabel PassarellaSilva, Debora MarcolinoVital, TamiresNitz, NadjarCarvalho, Bruna Caroline deHecht, MarianaOliveira, Diana Souza deOliveira, Edward Jose deRabello, Ana Lucia TelesRomero, Gustavo Adolfo Sierra2020-02-07T16:31:51Z2020-02-07T16:31:51Z2019TEIXEIRA, Ana Izabel Passarella et al. Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/3979910.1590/0074-02760180452engFundação Oswaldo CruzImproving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future testsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Universidade de Brasília. Faculdade de Medicina. Laboratório Interdisciplinar de Biociências. Brasília, DF, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Universidade de Brasília. Faculdade de Medicina. Laboratório Interdisciplinar de Biociências. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.BACKGROUND: Studies aimed at validating canine visceral leishmaniasis diagnostic tests present heterogeneous results regarding test accuracy, partly due to divergences in reference standards used and different infection evolution periods in animals. OBJECTIVE: This study aimed to evaluate the accuracy of the rapid test-dual path platform (TR-DPP) (Biomanguinhos®), EIE-Leishmaniose-Visceral-Canina-Biomanguinhos (EIE-LVC) (Biomanguinhos®), enzyme-linked immunosorbent assay (ELISA) rK39 (in-house), and the direct agglutination test (DAT-Canis) against a reference standard comprising parasitological and molecular techniques. METHODS: A phase II/III validation study was carried out in sample sera from 123 predominantly asymptomatic dogs living in an area endemic for visceral leishmaniasis. FINDINGS: Sixty-nine (56.1%) animals were considered infected according to the reference standard. For each test, the sensitivity and specificity, respectively, were as follows: TR-DPP, 21.74% [confidence interval (CI)95% 13.64% to 32.82%] and 92.59% (CI95% 82.45% to 97.08%); EIE-LVC, 11.59% (CI95% 5.9% to 21.25%) and 90.74% (CI95% 80.09% to 95.98%); ELISA rK39, 37.68% (CI95% 27.18% to 49.48%) and 83.33% (CI95% 71.26% to 90.98%); and DAT-Canis, 18.84% (CI95% 11.35% to 29.61%) and 96.30% (CI95% 87.46% to 98.98%). CONCLUSION: We concluded that improving the sensitivity of serum testing for diagnosing asymptomatic dogs must constitute a priority in the process of developing new diagnostic tests to be used in the visceral leishmaniasis control program in Brazil.Canine visceral leishmaniasisDiagnostic testsSerologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/39799/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALImproving the reference standard for.pdfImproving the reference standard for.pdfapplication/pdf9625704https://www.arca.fiocruz.br/bitstream/icict/39799/2/Improving%20the%20reference%20standard%20for.pdf054db17c6221e0bc14535e15eadfaa6bMD52TEXTImproving the reference standard for.pdf.txtImproving the reference standard for.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
title Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
spellingShingle Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
Teixeira, Ana Izabel Passarella
Canine visceral leishmaniasis
Diagnostic tests
Serology
title_short Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
title_full Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
title_fullStr Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
title_full_unstemmed Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
title_sort Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
author Teixeira, Ana Izabel Passarella
author_facet Teixeira, Ana Izabel Passarella
Silva, Debora Marcolino
Vital, Tamires
Nitz, Nadjar
Carvalho, Bruna Caroline de
Hecht, Mariana
Oliveira, Diana Souza de
Oliveira, Edward Jose de
Rabello, Ana Lucia Teles
Romero, Gustavo Adolfo Sierra
author_role author
author2 Silva, Debora Marcolino
Vital, Tamires
Nitz, Nadjar
Carvalho, Bruna Caroline de
Hecht, Mariana
Oliveira, Diana Souza de
Oliveira, Edward Jose de
Rabello, Ana Lucia Teles
Romero, Gustavo Adolfo Sierra
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, Ana Izabel Passarella
Silva, Debora Marcolino
Vital, Tamires
Nitz, Nadjar
Carvalho, Bruna Caroline de
Hecht, Mariana
Oliveira, Diana Souza de
Oliveira, Edward Jose de
Rabello, Ana Lucia Teles
Romero, Gustavo Adolfo Sierra
dc.subject.en.pt_BR.fl_str_mv Canine visceral leishmaniasis
Diagnostic tests
Serology
topic Canine visceral leishmaniasis
Diagnostic tests
Serology
description Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-07T16:31:51Z
dc.date.available.fl_str_mv 2020-02-07T16:31:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TEIXEIRA, Ana Izabel Passarella et al. Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39799
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760180452
identifier_str_mv TEIXEIRA, Ana Izabel Passarella et al. Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.
0074-0276
10.1590/0074-02760180452
url https://www.arca.fiocruz.br/handle/icict/39799
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/39799/1/license.txt
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